FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_48.35100000043145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_48.35100000043145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC6973.463354037267081RNA PCR Primer, Index 46 (95% over 21bp)
TGTAAACTGTAATGAACATCTGTGTAGTTAAATCTCACCTACCCCCTTAAT920.4571428571428572No Hit
CACAAACTCATATTGAGAGACTTTCTACATGATACCTTACCAGTACTGCAC910.4521739130434782No Hit
AATGTGTATAGTGCTGATTTACTCAAGAATGAAACAGGTAAATATGACATC660.32795031055900625No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT660.32795031055900625No Hit
ACATTAATGTAATGGAATCGGATGGAATGGAATTGAATGTATTGGAGTCGA620.30807453416149067No Hit
GTATTGTCGTGAAAAATTCCTCTGAAAGAAACAGCTTTTTCTTTTTCATAA600.2981366459627329No Hit
ACATGAATATTTTTGGCTCTAACAACTGCACCTTTGAAATGGCATGAAACG580.28819875776397513No Hit
AGAATATTTTCGTTTCTGTCGCATTTTAGCTTGTAAATTGAGTACTTTCAT580.28819875776397513No Hit
CATGCATACAGAATGTACTTGGCATTTTCTAAGAGAGTAGACCTCCTCACC570.28322981366459626No Hit
TGATAGAGGTGAGTCTTAAATTCAAATTTTCTGGCTACCAATTCCATATTA550.27329192546583847No Hit
ATGTCTATTTCATAGGTTGCTCTAAGGAATAAATACATACATGCAATGCCT470.23354037267080746No Hit
ATGATAATCAACAAATACGCAAACTTTCTCTAGAAGGAGCAGAATTACAAC470.23354037267080746No Hit
TAAATTGCGGATTGGTATTGATATCGGCATGTACTTCCATATCAACATGAT440.2186335403726708No Hit
GTATCATTCATAGAAAAACCAATTACCGTTAATACCGCGGCAATAAAATGC430.21366459627329193No Hit
ATACTACAAGGCTACAGTAACCAAAACAGAATGGTACTGCAAAAACAGACA430.21366459627329193No Hit
ATTACAGAGTGGGAACATTTTAGTAAAGGCATTGGGATGTTTAAATGCCAA400.1987577639751553No Hit
GTTAGTTTCTTATGGAAGTCTTACAGCATTTTGTGTTCTCACCCAGGATAA400.1987577639751553No Hit
TATAAACACAGACCAGAAAGCTGAGAAATACTCTTGATAAATTCTTCATCT390.1937888198757764No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTGCTTCTTCTTCTTCTGCTTCTTC380.1888198757763975No Hit
GAAGTATTCAGTGGGATGACATAGTGGAGTTCATCTCCCTAATGAATCAAA380.1888198757763975No Hit
AATGATAATTGGTTGATTTTTTCTCCAAATGATTGGAGTGTTACTCCTAAT380.1888198757763975No Hit
CTGTAGAGATACCAAGCTTATGAACTATTCTCCCAAAAACAGATTTGTTCG380.1888198757763975No Hit
TTATAGGAGTTCCCTTGTAATGACAAGTTCTTTTTCTCTTGCTGCTTTGAA370.18385093167701863No Hit
ATTGTTGATAGTATGTGATTGTCAGAAATCAACTGCTTCTCCTTTTTCAAT370.18385093167701863No Hit
AAATGTAAACTCATGGGTTTTTACATGATTACTAAGGGTATTGGCGTAAAA360.17888198757763973No Hit
CCAAATAATACAACGAGTGCAATACCAAATAGTATCCACATAATGCGTATT360.17888198757763973No Hit
AACTCCAACAGTTTTCTTGTGGATTAAACCATTGTATCTGAAACTGTTCTT340.168944099378882No Hit
GGAGAAATGGAAATGATCTTTAGGACACTGTTAATGTGCATAAAATTCTGG340.168944099378882No Hit
AATCTACTCTCAGCAATTTTCAAGTATATATCACGTTGCTGTTAACTCTAG340.168944099378882No Hit
GTTGTAAGCAATAGGTTTTCCATTTTTTACAACAACATTCAAATTCTGATT340.168944099378882No Hit
TTTCGATTCGATTCTTTTCGATTCGATTGATTCTTTTCGTTTCGATTAGAT330.16397515527950313No Hit
AAATGACTAGAAGGAAAACCTCACCTCAAAAGAAAAAATCAGAAACAGTCC320.15900621118012423No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC320.15900621118012423No Hit
TAGTAGATATCACCTTTAAAGCGCTTTAGCTTACTTGTAGAATCGAAGTTA320.15900621118012423No Hit
TTAGAAATTGAACAACATGGAGCAGAAAAAGTAGGATTTGAAGAATTGATT310.15403726708074533No Hit
AAATAGAACTTGGCTACTCCTTGAAAATCTGTTTTTCCTCAGGACATTTCT300.14906832298136646No Hit
ACCATATCATGTGTAAACAGAAGTAATTTTACCTCTTTGTTTCCAGTTTCC290.14409937888198757No Hit
GGAGTAAACATTGCAACTAGCCTAAAAAATCAAGGCTTTGAAGATCTAAAC290.14409937888198757No Hit
ATCATATACCACAACATTTGCAACAAGTGAACGCTGTGAAAAATCAAACAC290.14409937888198757No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1391304347826087No Hit
GGTTTTGGAGGTAATCTTCGAAAAGAAGTAGTGAAAACAAAAAAGTGGTAT270.1341614906832298No Hit
ACATAACCCTTGTTGACTTTTTCAAGGGAATTAAATAGCTACTCCTTTTCA270.1341614906832298No Hit
GAGGAAATTATTTATGTCTCAAACACAAACTTGGGAAAAACTAGAAGGTAA260.12919254658385093No Hit
AGGTATAGTCTACATAATGCTAGCCTTCTTCTAACATCTTCTCCTACCTGA260.12919254658385093No Hit
GAATAAGCATAAGGATCTCTTGAAATGTCATCTATTATCCCAGACTGTGCT260.12919254658385093No Hit
ATAATTCCATTCGATTCCATTTGAGGATAATTCCATGTGAGTCCATTCGAT260.12919254658385093No Hit
ATGCTGTAGAAGAAAAAATTGAAATTGCAAAACGACATTTAATACCCAAGC260.12919254658385093No Hit
GAATAGAATAGAATGGAATCATCAAACGGAATCGAATGGAATCATCGTCGA260.12919254658385093No Hit
GTACATATTCTTTGTGTGAAATTCATTGCAAAAGAATGGCTCTAATTCTTC250.12422360248447205No Hit
AACAGACATTGTTTTTGTGAAATGGAAACTCGAGATTCTAATCATAGACAC250.12422360248447205No Hit
TAATTCAACAGCTCAGTTAGGTGCATTAAAAGCTACTTTAGATCAAACAGA250.12422360248447205No Hit
GATATACGTGCTTGTGCAATAATTTTCTCACCAATTTCTGTTGGTACTATT250.12422360248447205No Hit
GATTCATTTTTTCTGACTTGATTTAAAGTCCTCCATAAATTCTTCTCACAT250.12422360248447205No Hit
GTGAAAAGAATCGAATCGAATCGAAAGAATCAAATCGAAAAAAAAATCGAA250.12422360248447205No Hit
GAATAGACAATTCTCAAAAGAAAATGAACAAATGGCCAACAAACATATGAA240.11925465838509317No Hit
TTGCTGAATATGTTGAACTGCTTCATTGATATCATCGGTTACCAAAACCAG240.11925465838509317No Hit
GACAAAGCAATTCTAAAAAAGAAAAACAAAACTGGAGGCATCACAATTCTG240.11925465838509317No Hit
ATAGTACGAATCAGAGCTTGCATGAAATTGTTTTCATCTACAAATTTAAAG240.11925465838509317No Hit
ATTTTATCATCTGTCTCAGTTCTCTATTGGCTTTGCTTGATTGATTTTTCT240.11925465838509317No Hit
AATGTGATGTCTCCAAATTTGTTCTTTTTGCTTAGTATTGCTTTGGCTATC230.1142857142857143No Hit
AGGTAAAACCACTATTGCACAAATAATTGCGGAAAGCCTTGGTATGACTTA230.1142857142857143No Hit
AATCAAAGAAGATGGTTCTGAATCATCTATTGGTAAATTCAAAACTGACAA230.1142857142857143No Hit
TTATAACAACTGTTTCTGCAGCAATCATGGAATTGATTGATACATCTATTG220.1093167701863354No Hit
AACTCCAATTGGAAGATTAGTTACCCGTAATATTTCGGATATCGAAACTTT220.1093167701863354No Hit
TGTAACTACTGGTGAACCATTATCCATCCCTACATGAAAATTGTCTTGGTC220.1093167701863354No Hit
GCTTTAATTTAGGTCTTTGAGTTATGTAAGCAGAATGCAATTAAAGAAGAA220.1093167701863354No Hit
TCCATGTACAAATTGGGGTTAATTATGGAAACTACCTCGTTCAAGACTACT220.1093167701863354No Hit
TCATTGAATGGAATCAATTGGAATCATTGAATAAAATCGAATGGAATCTCC210.10434782608695652No Hit
ATCAAAGCTTACTTGAAATAATCCGTTACTTGGATTTGGTGTAACTTTTAA210.10434782608695652No Hit
TGGTTACATCATGATGATAAAACTCAAATTGCTCCAGTTTAAAAAGGTGTT210.10434782608695652No Hit
TAACTTCAACATACATCCAAAATCAACATACTGGATCAAGTTCACAATTAA210.10434782608695652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1050.040.71428745
AGCCCAC1250.037.80000317
CGAGCCC1250.037.80000315
CCACGAG1200.037.49999620
CACGAGA1200.037.49999621
ACGAGAC1200.037.49999622
CGAGACC1200.037.49999623
AGACCAT1200.037.49999625
GCCCACG1300.036.34615318
GAGCCCA1300.036.34615316
TCTCCGA1300.036.34615311
CTCCGAG1300.036.34615312
CATTCCG1250.036.00000429
AGAAAGA250.002058341436.00000415
TCGTATG1250.036.00000440
ATTCCGA1250.036.00000430
CTCGTAT1250.036.00000439
AATCTCG1200.035.62536
ATCTCGT1200.035.62537
TCTCGTA1200.035.62538
Produced by FastQC (version 0.11.2)