FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_47.3510000004311e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_47.3510000004311e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249076
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC98003.934542067481411RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT12570.5046652427371565No Hit
ATTGTATCACATTCCAGTTTGTAGAGTAGATCATTTGATCTGTCTCTTATA6690.26859271868827184No Hit
GAAGTGTTATGATGTACGATTTAAACACAGGAAGAGAAACAGTTGTAGGAA6490.26056304099953426No Hit
GAACAAATGATCTTCTTGTCTTCTGATGTCGTTTTAACTTTGGAGAAACTT5830.23406510462670027No Hit
TTGCAGTGTAATGTTGTATGTATAACCTTTGTTGGTATTGGTCATCAAAAT5600.22483097528465207No Hit
TACCGTATGTTTCTATAACTTGTGAAAGCACATCATTGATACGCTCAGAAT5590.2244294914002152No Hit
ATCTTGGATGGTTTCCATTAGCTACTATGTTATCAATGCTCTGAGTATCTA5560.22322503974690455No Hit
ATAGTAGACAAACTGTAATGTAATCACTGAATGGACTCTTACGTTTCAGTG5210.2091731037916138No Hit
ACAAAGTTCATCCAAAGAGTAAGCATTTTGCTAAGAAAAGTTTACAAACCT4840.1943182000674493No Hit
TCTCTGAGTTTAAATGACAGCATACTTTCTTGGAGTTGAAACATCCATTAT4830.1939167161830124No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG4760.19110632899195426No Hit
GTAATGAATAGAAGCTTGTTGTGTTACTCTAACATGATCATTTGAACCACT4740.1903033612230805No Hit
GTATTTCTCAAGTGAGAGCAATAAGATTTTTTCTACTCACATTTGCTTTGG4620.18548555460983798No Hit
AAATAGCATGCTTTACAATGTCACTTACACAACATTGGAGTTTTTGATCAT4600.1846825868409642No Hit
GAATACTCTTACACGTTGCATTACTTGTCATTACACATGTAATTGAATCTG4600.1846825868409642No Hit
CTAATTGACTTACATGAGAAGAAGCAGGGTAAAAAACTTTTTAGTCAAGCA4400.17665290915222662No Hit
ATATGCAACATATCTCCGATCTCTTCATGACTATACCCCTCAATGACACTT4330.17384252196116848No Hit
TACAAAAGCACTTAGAACTGAAACATTAAGCACGAAATCAATTTTAGAAGC4120.16541136038799403No Hit
GTGTTAATCAGAGTGCTTTTTGTTAACTGCCAGGTTTTGGTTATATACCTC4120.16541136038799403No Hit
ATGTACAGTTGTTCTCATAAGAAACGGTTTTTAGTTTTACAAGGATTTGGA4120.16541136038799403No Hit
GTATTGACTAGGCAGTTAAATCAAGTTTTAGAGACTGAATGTTACTTAGCT4090.16420690873468338No Hit
GAGTTAAGTTTATACTCACAGCATTTTTACTACGGACGATATGATATCTGT4090.16420690873468338No Hit
AGTATGGTGTGTACAAAAGGAACATAACCATTATTTGCAAGTCTTTGCAAT4080.16380542485024652No Hit
ATACAATACTACCTTAACCTTTCTAATGATCTGATACCTGTCTCTTATACA4050.16260097319693587No Hit
CAAATAGCATGTGTGATTTTTTTCAGTGTTGTTTTTATCGTATCGTAGTTC4010.16099503765918835No Hit
GTATTGGGTATTTGTTTAGGAATGCAGTTGATGTGTAGCTATAGTGAAGAA3990.1601920698903146No Hit
TCTTTACCCAGATACTCATTCCTCTGTCACCTAACTTCTTGAAATTTCATT3980.15979058600587773No Hit
AGTGTATTCATTACTTCATCCGTTGATAAGACAGTTGCTACTTTTAATGTA3980.15979058600587773No Hit
ATCACACACACCTCTCCTTTTACTCGTAAGTGTACATCTTGTAAACATGTT3970.15938910212144086No Hit
AGCTATTCCAGTTAAAACAGCTACATGGGGATATAGAAAATGAAACTTCGG3950.15858613435256708No Hit
ATCACAAGTATGGCATGTCTTCTGTAATTTCTCATAAACATGGGTGTCATC3880.15577574716150894No Hit
TAATTGAACCTTTCGATTGATTGAATACATTAATGCAGCATTCATCAATAA3880.15577574716150894No Hit
AGGTTGGTTCATACAAAAGTACAGCTACTGTAACTGTTTCACAAATACAAA3840.15416981162376142No Hit
GTACAGTAGGGGTTTGTGTTAGTACACTATGATATTCACACGATTATGAAA3800.1525638760860139No Hit
ACTAATCCTCTTGTTAATGAGCAATCAATATTAACCACAACTGGTTCTGTC3780.15176090831714015No Hit
ATGCTATTCCTATCATACTACCAATGACATTCTTCACAGAACTAGAAGAAA3710.14895052112608198No Hit
CTCTAATGTTTCTTCTTTTTTAGCAAAACAGAAACGAAGCACTTTATTATC3670.14734458558833446No Hit
GAATGGAATCGCTTACTAAGAAAGGTAGATTTTGATTGGATGGATCAATCA3570.1433297467439657No Hit
TATGTATCCCATTGATGTATCCCATTATAACAGTCAGAGCAACTAGATCTG3510.14092084343734443No Hit
AGATTGGGTGATGATATAAGAAGGATACCAATCCACAATGAAGACCTAACG3500.14051935955290754No Hit
TACCTAAATCCACCAATGGATTAGAATCATTTTTCGGACATTTGAAAAACC3500.14051935955290754No Hit
GATAATACTTAACCACTTCACATAGTGTTTCAAGGTCAATTAATATCTGTT3500.14051935955290754No Hit
CTTGAAAAATAGGTTTCTTCGCTTTTCTGAACAAAGGGTATCTCTTCATTT3490.14011787566847067No Hit
CCTATGTCTATGTCTACATCTATATCTACATCGAGTTCTATTTCTACTCCA3410.13690600459297564No Hit
CTGTAACAACATCTGTAATGATCTGCATACAATGCCATACATTCACATTTC3380.13570155293966502No Hit
CTTTAATAAGCTCTTCTTGTCTACTTTCAAGTCCAAGTTTTTCAGATTCTT3370.13530006905522812No Hit
ATGGATTCCTATTGATCACATGGATTGAGCTATAACTTTGTACATGTACTG3360.13489858517079123No Hit
ATACTGATTACAATCCTTGTATACATTGGCATACAGCTGTCTCTTATACAC3320.13329264963304374No Hit
CTTTCTCTAACTGCTTCTGAAACAGAGTCCTAGACACATCTACTCCTTGAG3270.13128523021085933No Hit
ATGTAACAGACCAGGAATCTTTCAACAATGTAAAGCAATGGTTACAAGAAA3250.13048226244198557No Hit
CAGATTTTTCAAAAAGCGTGTTTCAAAATTGCTCAATGAAAAGAAAGGTTT3220.12927781078867495No Hit
GTATAAACACAACACACGACAAACTATTCTAACGTATTCACACATTGACAG3220.12927781078867495No Hit
ATTCATCACTTCGTAATTCTCAGCTTTTACATCTTCTACACGGATATTACC3200.1284748430198012No Hit
ACAGTAACATAAGCATTATCATCATTCGTACTTAAAGAACGGTCGCAATAG3150.1264674235976168No Hit
ATAGTACAACTTTAATTCTGCTTGTTGATGGTGTTAATGTGTATGAGAGCC3130.12566445582874303No Hit
TGATCACACTGGATTTCATTCCATAGTTCTATTCGATTCCATTCGATGATG3110.12486148805986928No Hit
ATTGAATCTGGCTTAGATATTCCTAATGCCAATACCATCATCATCAATTCA3100.12446000417543239No Hit
TTCTTCACTAGTACCTACATTTTGTCAGAAACTGCGATATTTTTCAGACTG3090.12405852029099551No Hit
TGTATATAGACAGAAACATATGCATTCACATACACGTAATGATAATGCTGT3090.12405852029099551No Hit
TCCAAGTACATTGTCAACCCTGTAAATAGTGAAGCAAAGTTTATTCAAGAT3080.12365703640655865No Hit
ATTCTTCAGAGTAACTTTAAAATCAACCTGCCAACTTAAACCCTGTGTTGA3070.12325555252212177No Hit
ATTCAAATCATTGGATGGATATATTCATGCAACCGATATACAATTGGCTGG3060.12285406863768487No Hit
ATTTATGAGGCAATAGTTATGACAAAGGCATAGTAACATAACTGATTTCCC3020.12124813309993736No Hit
CATGAAGTGAAATCAGTTTCAACACAACAGAAAACAAAGCTTAAAACATTG3010.12084664921550049No Hit
GATGTGTATAACTAAAGTGTGGTTAGTAAAATGATTCTGCCACAAACAGAT3000.12044516533106361No Hit
TTCTTACATAAGATGAGTCTCCTTTAGTTAAACTGCTCATCATCCTGCTCA2980.11964219756218986No Hit
GTTATTATTCATGCGACAAACAAGGTAGTCTTTAGATTCAACACGACACAA2980.11964219756218986No Hit
AACTTGTATCCATCATGATCACAAGAAATCCTAAATGGTTGATAATGTCAA2980.11964219756218986No Hit
CATATATGATCATACCGAAGCAATGATTCTGTTAGATGTTGATTTTCAGCA2960.1188392297933161No Hit
AGTAGGATAAGCTGATGAGCAACTTAATGTCTACTTTCTATGCTAAGAATC2950.1184377459088792No Hit
GAATTAGTTTTAGTTTTGAAAGCTTTGAAATTCAAGAATGAGTGTTGTAAA2940.11803626202444234No Hit
GAACAAGTGTTCTTCTTTGTCAAATGCTTTTTCTGCATCTATTGAGATGAT2940.11803626202444234No Hit
GTTAATTAAGTCACAAACTTAAGTGAAAAATAAGTTCCTGTCTCTTATACA2930.11763477814000546No Hit
TCATAGCAGAATGTATTGCACATGTGTTTGTGGTGATGCTAATGTAAACCT2930.11763477814000546No Hit
ACATTATGCCTGGTAGTAGAAGTAACAGAACATTACTGTCTTATTTGCAAA2920.11723329425556857No Hit
ACATAGGACATTACTGAAATGAGCATGTTACCTCGTATATCATCCTGGTTA2900.11643032648669482No Hit
GAATTGAATGGAATCATAGAATGGAATCCAGTGTAATCATCATCGAATTGA2870.11522587483338419No Hit
CAGATATCAATGCCTTATGTGATGAATATTTAAGACTGGCGTATCATGGAT2860.11482439094894731No Hit
TATCGATTCTACGAAATACTAACCACTATACACTAACTACCTGTCTCTTAT2840.11402142318007356No Hit
GATCAATGTATTATGGATAGGGATCGTGATTATGGTACTGGGTTTCATATC2830.11361993929563667No Hit
CTATTTCTTCATCACTTTTCTCATCAATAACTGTGTATGATGACTGTCTCT2830.11361993929563667No Hit
GCGTAAGGTTTCATTTTCCTTTTTGAGTTCTTCATTTTGATGCTTAAGAAA2820.11321845541119979No Hit
CAGATAATCTACAGAATGGGAGAAAATTTTTGCAGACTATGCATTTGACAA2820.11321845541119979No Hit
ATTTAAGTGGGTTGGTAGCTACTAATACAACATTGGATAGATCGAATCTAT2800.11241548764232603No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAAACCATACGATCAAATGAACC2780.11161251987345228No Hit
AGATATGACATGTACTTGGTAAAGGAACTGATAATATAAAGCAGTGGCTTA2760.11080955210457852No Hit
GTTCTTCTTCAAAAAGTGCTATTCAGAATACGAACTAATTGGCTACATACA2760.11080955210457852No Hit
CTACAATACAAAGTGTTATGACGTCAAATACGTAATCTACCTGTCTCTTAT2750.11040806822014164No Hit
ATGTACACTGTGGTAATAGCGAATACATGCTTACAGAAGCTTGTGAATACA2730.10960510045126788No Hit
CAACCTTTAGTGATCATCTTAGCCACAAATCTTTCTTAGATGACTCCTCAA2720.109203616566831No Hit
AAGACACATACATAGGAAAGTACAGAGGAATATAAGTGCTGTCTCTTATAC2720.109203616566831No Hit
CTTTTATTGTGTTTCACAATGCAATTTTGTTGTTTTTACACCTGTCTCTTA2720.109203616566831No Hit
GAGATTGAAGCTCTTACTGATAAAGTGGAAGAATTGCAAACTGATCTTGAG2710.10880213268239414No Hit
GATGTATTACAAGAGTTGGAGCCTATTCATTTTGAGGTTGTTTTTGTTACG2700.10840064879795726No Hit
GCATACAACACAGTGTTAAGCATGGATTCATAGAAGAGACAATATCCCATC2690.10799916491352037No Hit
GTGTGATATTAAAATGCCCAAAGTAGATGGCATTGAGTTTCTTGAAAAGTC2690.10799916491352037No Hit
GTAGTATACGGTCTATTCAAAGCTGACAAAGAGCTTATAGTTAGTTAGGTG2680.10759768102908349No Hit
AAGTATGTGGGAAACAAAGTCACTCACTTTGAAAATGGTACATATCAAGTC2670.10719619714464662No Hit
ACACATAACAGTGAAACTTGCAAATCTCAGAGACAAAGAGAAAATCTTGAA2660.10679471326020973No Hit
ATCCAAATGGCATTGGGAGATTATTTAGACAAAGCAAAAAACTTATTCTTA2660.10679471326020973No Hit
ACAATTGGGAACGTATATTATCGATTTAATCCAGCTCAATTCTGGACTAAT2650.10639322937577285No Hit
ATTTGTAACCTCGTTATCGTTTAATATTCGTAACCTAATTCCGTCAAGAAA2640.10599174549133598No Hit
CATATTTTGTGAATATCTTGCCTGTAAAGTTTGTTTCGTGATAGCTTGTAG2640.10599174549133598No Hit
TGTTTGGTCTATTTGTTCCATTTCTATGAGGGTCTTGTTTTAAGTGCTTCT2600.10438580995358847No Hit
TCATTGCTCTAATAATCTTCTGGTCCTACTATGCCTATGTTGTGGAGCTCT2600.10438580995358847No Hit
GAAAGTACTTGTGGTAATAATGGAACAGCAACTATAGTTGCAACAAGTAAT2590.10398432606915159No Hit
ATACTAATAACAGCAGCAAACGAAAGTTGAAAACCAATATCTTCTATCAAT2580.1035828421847147No Hit
ATTTAAACTGATTCAAGGAAATGGACCAATTCATATTGTTGGAAACCACAT2560.10277987441584095No Hit
TTGAAAGCATGTTAACTGAAAGAACTCAGGCAAAAAAGACTACATATCTGT2560.10277987441584095No Hit
GTACCCAACCATTTTTTTTCAACTTGTTTAGTATCTCTGTTACTTTGCCAT2550.10237839053140406No Hit
GAATGTACCCTCAATGAGAAGACATCAGTATGTGGAAAGAGTAACTTTTAA2540.10197690664696721No Hit
AAAAATGAGTTCCAACTCCCTTTCATATGGTTGGGATACTCAAATACATTT2530.10157542276253032No Hit
ATCATAGCTACTGTTATTTACGCTTACTGCTCCTTTATACCAATATCTGTC2530.10157542276253032No Hit
ATGGTAGATTATTTTCAAGACAATGAAAAAAGAATCTGCAAGTCCTGTTGA2530.10157542276253032No Hit
GAAAAACACGTATCAAAATAGGTAAAAGTTGAAGGTAAATGCCTGTCTCTT2530.10157542276253032No Hit
AGTCTCAGTCTTCTTAGAGAATACCTAAGTGGTTGTGAACAGTGTTAGTAG2520.10117393887809344No Hit
AAACAAATGCTATCATTCACTGTACTCTCACTCACAATGGTGATTTTAAGA2520.10117393887809344No Hit
GTATTCAAAAGACCTTCAAGAAGTAGAATCAGTAGGACATGATTACTGTTA2510.10077245499365654No Hit
GGTATTACCTTTGCCCTCTAAATTATTGCAATGACGATTCATAGAGAAGGG2510.10077245499365654No Hit
ATGAGAAAGTGTTCACATAATTGTACGCAATGATGAACACCCAGAATGTTG2510.10077245499365654No Hit
TATGAAGCTATATCGTTCGTTTAGAGAAGAAGTTCCTGAAGAAGCTTATAC2500.10037097110921968No Hit
ATCAATGATAGACTGGATAAAGAAAATATCGCACATATACACCATGGAATA2500.10037097110921968No Hit
ATACCATAGGAAGGGCCTGTATCCATTTTAATCCTGTCTCTTGACATACTT2500.10037097110921968No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG207.016616E-445.007836
TATGGGG207.016616E-445.007833
TCTTAGT207.016616E-445.007834
GGTCACT207.016616E-445.007833
GTAGGGG551.8189894E-1245.007836
TCCACAG207.016616E-445.007835
AGCTCCG207.016616E-445.007835
TCGGTGG207.016616E-445.007832
TGATCGC302.1590313E-644.99879537
TCACGGT302.1590313E-644.99879540
CGGAATG253.8825936E-544.99879512
TTCTACG302.1590313E-644.9987957
AGCGTAC406.7793735E-944.99879519
AACCGAT302.1590313E-644.99879531
GGAGCTC406.7793735E-944.99879544
GCCCAAG253.8825936E-544.99879527
CCGGGAA207.0235616E-444.99879538
CGAACGG253.8825936E-544.9987958
ACACGGA406.7793735E-944.99879538
ACTGCGT253.8825936E-544.9987958
Produced by FastQC (version 0.11.2)