FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_46.35100000043101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_46.35100000043101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224701
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC114115.078304057391823RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT14480.6444119073791393No Hit
TATTGTAACTTTGATCAAGGGCAAAGTTGAAGAAGTTGTTCTTCCTGTTGA5630.25055518221992784No Hit
GTGTTATACACATACTTGACAACATTGTGATAATATGCAAGTTCTAACAGT4800.21361720686601304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG4250.18914023524594906No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTACATTCCATTCCATTACA4170.18557994846484885No Hit
AAGTTACATGGACAGAAATAGAACTGCATTTTACTTCCTTTTGTCTTAATT3860.17178383718808551No Hit
TTCTTTTGTAATCTTCACAAGTTGTATCACATCTTCTTTGGCTTCTCCAAT3830.17044872964517294No Hit
TGTTTGTGGTTTCTATGTTGTACTATCGTTCATAGAAACTCTGGTATTTTT3730.16599837116879765No Hit
ACGTATTTTCATTTAAATGCTCAGTGACATTTACTTACCTAACAGGTTCAT3150.14018629200582106No Hit
GATGTTACCGTATTGGACAGAGAGATGTTGTTAACGTTCATTACCTCATTG3090.1375160769199959No Hit
ATTCTGAACACAATAGGCATAAACTGTAAACATGATAAGGATAACAAACTA3050.1357359335294458No Hit
GATATGGACTAGAATAGAATGGAATGGAATAGAATGGAAAGGAATGGTTTC3030.13484586183417074No Hit
GTTCTTCCAATTCCACTTCAGGATTTGTTAGTGCATATTCTCTCCCTTTTG3030.13484586183417074No Hit
AAATAGGTTTTGTAGCAACAAGAATACGTCGTTCAAAGTTCTTAAACAGTT2970.13217564674834556No Hit
GATGTACTTCAACAGATGAATGGATAAAGAAGATGTGGCATATATGGCATA2950.13128557505307054No Hit
CCAATAAACAAGGAACAGTTGATAAAGAGATAATTGGTTGAGCGATAAAAT2950.13128557505307054No Hit
GATGTACATATCTCTTGCTATTGCATCAGTTGAAATATCATTTTTTCCCGG2940.13084053920543298No Hit
TAGTATTATGCTCCAAGTATATATCCAAGCTGATCAATTCATAACACTTTC2910.12950543166252043No Hit
GCTTTCAATTCGTTGTATTTACAACCTTGTGATCTCACATACACAGAATCA2870.1277252882719703No Hit
GTATTACATGAGTTTGGTTCGCAAATAAACAGAACTCAATGTGTTTTGCGT2800.1246100373385076No Hit
AGTAGGTATTAAGACCAGGATTTGTAAACAGAATTCCATTTAAGTAGCGAT2720.1210497505574074No Hit
GTTTATGCTACTGCCTTAGAGTTTTCATATACGCAAGCTCCATTGCAGATG2700.12015967886213234No Hit
AATGTAGTTAATGTATGATGCATTAAACAACTCAGGTAAGAGCTTAGAAAA2700.12015967886213234No Hit
ACTTCCAATATTGTGTCATTCACGTTATAATTGCCTGGTTCAGCAACATAG2690.11971464301449482No Hit
ATCTCTAACAGTGCAATGATTATGCAAAACATAACAACAAAATACACACTG2610.1161543562333946No Hit
ATAAATGTCCTTATTTGTTGAGGAAAAAGGAAAGTAAGAGACCTAGTATTC2610.1161543562333946No Hit
AAGTAACAGAAGGCTATTTGTAAGAAAGATAAAGGCTTGTGAACAAAGAAA2590.11526428453811956No Hit
AATCCTGATAGTCTTAAATATGGTTTGATCGTGTTCATGTTAGATTAACGC2540.1130391052999319No Hit
TTGTATACCAGAAAGCAATGGATATTTGTTCATGTCTACATGCATATACAC2520.11214903360465686No Hit
ATTCAAAGTGTAAGAACAGCAAACCTAACTAAACAAAAAGGGCTTTAGTTT2520.11214903360465686No Hit
TCTTAAAACAGAACATCAACATACAAAACAATGCATTAAAAACCTGTCTCT2520.11214903360465686No Hit
TGACAAGTATGCACATGAGAAATATTCAAAGGAACGTAACACAAGTCGTTA2510.11170399775701932No Hit
GAATCAATCCGAATGTAATGGAATGGAATGGAATGGAATGGAATGGAATGG2510.11170399775701932No Hit
ATATAATCCTTGATGAGCTCTTGAATTAAAAAATCAAAGAAAGCTACACAG2490.11081392606174427No Hit
CTTTTATGTTGAATGGCCTTACTTTTATCAAGTCCATAAGCACATAAAACA2490.11081392606174427No Hit
ATTTAAAGGGACATCATTTATAGGGACAGACAACCCAGCTTATTCTCTTTT2470.10992385436646923No Hit
GTATAACAAACAGTATGATCTTTTTACTGTGATCATATCAAAGTTTCTGGC2470.10992385436646923No Hit
GAATGGGATCATCATCAAATGGAATCGAATGGAATCATCTAATGAACTTGA2400.10680860343300652No Hit
ATCATGATCATTGAAGAACCGGAATTCTATCAAGTGATAATTTGTGTTTTG2380.10591853173773148No Hit
GAACCAACTTAACATTACGTTTCTTCAAGGCGTAATTTACAACTTCCTCGT2380.10591853173773148No Hit
ATCATGCAATTTTAAAAACCTTCCTTTCAACTTCTTTCAAAGCTACTGACT2340.10413838834718137No Hit
TTCTGGAATTTACAAATTGGCATGAAATTATCAATAAGTCTGTCTCTTATA2340.10413838834718137No Hit
GGTATAGCATGCCTTAAATGAACGATGGTTAAAAGTATTCAACTTAAGTTG2320.10324831665190631No Hit
TCTATTATCTGAACTTTACATTGTCATGGACTGAAACTAACAGTGCAGATA2310.10280328080426877No Hit
ATTCATTGTTTGAGCAACAATATTTGCAACCTCCTCACAAGCAAATTCAAT2290.10191320910899374No Hit
GAATTGAATGTGCTCGAATATAATGGAATCGAATGGATTGATATCAAATGG2260.10057810156608114No Hit
ACCAATTACTAGTCCAACCACAATCATGGTGAAACTAAAGAGATTTAATTT2250.10013306571844362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG406.7466317E-945.020043
CCCCTAG253.870562E-545.020045
TTTAACG351.201879E-745.020043
GTTAGTA207.005771E-445.020042
AGGGTAC207.005771E-445.020045
AGTATCG207.005771E-445.020045
CTAACAC207.005771E-445.020043
AGCCTAT207.005771E-445.020041
CAGACCG207.005771E-445.020044
CAGAACC351.2060991E-745.00000420
GAGTGCG302.1576816E-645.00000434
CGAGTGC302.1576816E-645.00000433
TGCCTGC302.1576816E-645.00000425
CAGTGAC302.1576816E-645.00000422
CGTAAGC302.1576816E-645.00000427
AACTAGC351.2060991E-745.00000432
CGCTTTC302.1576816E-645.00000444
GTAGTCT302.1576816E-645.00000423
AGCACTA302.1576816E-645.00000433
TCTGCCG207.0211606E-445.027
Produced by FastQC (version 0.11.2)