FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_45.351000000430d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_45.351000000430d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135971
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2119215.585676357458574RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT1445810.63314971574821No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG17561.2914518537040987No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6880.5059902479205125No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5920.4353869575129991No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2610.19195269579542698No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2050.15076744305771084No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA1710.1257621110383832No Hit
GAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATT1610.11840760162093389No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1500.11031764126173964No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA1360.1000213280773106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.0006057E-445.013982
CGCCTAC207.0006057E-445.013983
GCCTACT207.0006057E-445.013984
GTCGAAA207.013312E-444.99742533
GGAGTTT207.013312E-444.99742522
TCTCACG207.013312E-444.99742513
GGCTGTG207.013312E-444.99742528
TGTGTCG207.013312E-444.99742530
TGTCGAA207.013312E-444.99742532
GCCGTCT253.873931E-544.99742545
GATAGTG207.013312E-444.99742525
GGGATAT207.013312E-444.99742513
TGCCGTC22800.044.50403245
CGGGATT356.210179E-638.5692217
ATCGCCT250.00209360436.0244371
TGATAGT250.002101183935.9979424
ATGATAG250.002101183935.9979423
ATAGGGG250.002101183935.997949
CAGTTGC250.002101183935.997949
TGGGTCT250.002101183935.9979445
Produced by FastQC (version 0.11.2)