H23LNBCXX_l01n01_bir_1228_pcontrolb_44.351000000430cc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_44.351000000430cc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211787
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	7971	3.763687100719119	RNA PCR Primer, Index 46 (95% over 21bp)
GTCTTCAATATTGTCAGATTTCTTGCGTTTTCCTGTATCTAAGCCTTGTAG	1222	0.5769948108240827	No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT	778	0.36735021507457966	No Hit
ATTCCATGGTGTTTATTGCAAGATAAATCAGTGATATCATCGTAGACTCAG	728	0.3437415894271131	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	724	0.34185289937531577	No Hit
AATGAAAGGAGTCATCATCTAATGGAATCGCATGGAATCATCATCAAATGG	498	0.23514191144876692	No Hit
ATTAGTGTGTGAACTAGAACCCAAATAACCAAGTTGCTGAATTAACACATC	473	0.22333759862503363	No Hit
GTTTAGATTTAGATCTTTCTCACACTTTTATGCTTAAGACCATTTTAAGCA	459	0.21672718344374298	No Hit
GAGAGAAGAAGATGGAAGTGATTGAAGTGTAGATGATTGAACAGAGGAGAA	437	0.20633938815885772	No Hit
TTTCTGTACAATTACTGTTACATTTACCCAGAATGGTAGCAGTACATACAA	429	0.20256200805526306	No Hit
CATATTGATTTCTTTTCAACACTTTGGCTTTTTTTTAGGAAGAAACTTAAT	380	0.17942555492074583	No Hit
TCATTCTGTTGATGTGATGTATCACATTGTTGATTTGCATATGTTTAACCA	377	0.17800903738189786	No Hit
AAGTACTACTATCTTCATGTACCCATCAAAATTGATCAATGGCCAATCATG	375	0.17706469235599917	No Hit
ATTCCCACCTATGAGTGAGAATATGAGGTGTTTTGTTTTTTGTTCTTGCGA	372	0.1756481748171512	No Hit
ATCATCATCAATTGGAATCGAATGGAATCAACATCAAATGGAATCAAATGG	341	0.16101082691572194	No Hit
TCTTATTGTTGTTTGTAGGTAGTGGTGATGTGATCGATGTATTAAAAGCAT	334	0.15770561932507662	No Hit
CAACATAACCATACAAACATTGCATGATCAAGAACTAAACATGATTTGTTT	316	0.14920651409198865	No Hit
ATGTTATACATTAGGACCATCACATGTTCTTCTCTTTAATCCCCAAACTTC	305	0.14401261644954602	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG	300	0.14165175388479936	No Hit
TTTTCTTGTGCCTGAATAAAGCTTTTGTAGTACTTCATTGAAAAGAAACAC	299	0.14117958137185002	No Hit
GTATAAACTCAACACCTGAGAAAGGAGAATACGTATCAAAGAACAATGTAT	292	0.1378743737812047	No Hit
CATCAAATGTCAATACTTGTGTGTGTGTGTGAAAACGTTTCAAGTCATGTT	282	0.1331526486517114	No Hit
GTTTTAATCCATTGTAATCCATAGGATTATGACTTGCAGTTACTTCAATAC	281	0.13268047613876205	No Hit
ACTAAACTCAATGCCTAAAAGACAAATAATCCAGTTAAGAATTGGGCAGAA	278	0.13126395859991408	No Hit
ATATTGAGGGAAGCACAACAGAGGAAAGAAAGAGAAGAAGAAGAGAAAAAA	275	0.12984744106106608	No Hit
AAATACCATTTTAACACTTCATGTGTACTCTAGGTCCCTCATATCTGAGTA	273	0.12890309603516742	No Hit
GTCATCATCTAATGGAATTGCATGGAATCATCATAAAATGGAATCGAATGG	268	0.12654223347042076	No Hit
TTATATTTCCTTTACTCCTTTCTTCCTTGCTTATTGTCTTCTTTTGTAATT	267	0.12607006095747142	No Hit
TGGATTTAGTTTGCATCATGATTCTCTGTTTTAACCAATATCAAGCATTTT	264	0.12465354341862343	No Hit
GTTTTTAGGTGTCACTTGGAATCAAAACTCTAGTTTGAGATATTTTTGTTG	261	0.12323702587977543	No Hit
CATCAGATTCTTCATCACTTTCTTTGACAACTTTTTTAGCCTTACTTTTAC	260	0.1227648533668261	No Hit
TTCAGAATAGTGTTCAGTAGCAATGTTGAATGTGAGTATGCTTGTCTTGTA	258	0.12182050834092745	No Hit
GTTCTACATCAAATATTTGAGCATCAGCTGGGAGAGAATTTTTTTCTTGAC	256	0.12087616331502879	No Hit
GGTTTTATTGAATCCAGTAACTGACAACCTGATATATTGTTTTCGCTTTTA	252	0.11898747326323145	No Hit
AGTTTTAGTTGGTCTTTTTTTTCAGGCATTTCTTCCTGTTTAACTTTATCG	245	0.11568226567258615	No Hit
AATTCATAGTCTGTGAACATCATGCTTGAACTCTATTCTTTCTTTTAGATC	245	0.11568226567258615	No Hit
ATCACATTTATTGGTTGCACAAGATTGCTTAAAAACCCTAATTGGACAAGA	245	0.11568226567258615	No Hit
GAAGTAATCTGATATTTGCTTCCATAACATTGTGTCATCTTCATTTGATAA	242	0.11426574813373815	No Hit
TATTAGGTATTTAGAGGAAGCATTTCTTACAACAACACACAAAAGCAAAAG	241	0.1137935756207888	No Hit
GAAGAATGTTTCTAAAAACTTTGGATTTGTGTGGGTTTCTCTATAATCTCG	241	0.1137935756207888	No Hit
AGATATTGTGCAGACTGAAGCTATTAAAATGTAAGCAACACAAATAGTAAA	241	0.1137935756207888	No Hit
AAACTATTCTGTGTGTTTAGGCATCAGAAATTGTTGAGTATGAAGAAGATG	239	0.11284923059489015	No Hit
AAATAACCATGAATCTGGTAAACTACAAATGAGATTTGTTCATTTCCTGTC	238	0.11237705808194083	No Hit
ATAATAAGCCTTGGTTTGATGTAATGGCTCGGGATGATTATTGTTATTCCT	236	0.11143271305604216	No Hit
GTTTACTCTTGTTGTTTTTGTCCTTTAAGAGGCATTGTTAGGTTGTTTATT	236	0.11143271305604216	No Hit
TAGATAGGTAGGTAGATATGATGGGTTGACAGATGGACAGTTGAATACATA	235	0.11096054054309283	No Hit
AATGAGAGGTGATTCTGTTGTTGTTGATGATAATGTAGTTTCAAAACGTGG	234	0.11048836803014349	No Hit
GATGAATGTGGTTACGAAAATACAAAGAAAAAAAACATGAAGAGCAACAAA	233	0.11001619551719416	No Hit
AGAAGAAGAAGAAAAGATACGAGCAAGAAGACTAAACACGAAAGCGATTTA	230	0.10859967797834616	No Hit
ACATTGTGAAGAAAAAGAAATCATCTTCCTCAAAGAGGAGGTGCGAGATGA	229	0.10812750546539684	No Hit
TAATTGATGAGTTCATATATCAGTTCCAAGCCTTCACTCAATATCGATGCA	228	0.1076553329524475	No Hit
AACAAATACTTACCATTGTGTTAAAATTACCTACAACGTTCGATACAGTAA	227	0.10718316043949819	No Hit
TATTCACACTTAAAGGATGCACATTCGATACTTTTCTCAGAAGATAGGACA	226	0.10671098792654884	No Hit
GATTATGATCAGAATCAAGACTTTGATGATGATGACGACGATTATAATAAC	220	0.10387795284885286	No Hit
GTATAGAAACATTTCCATTTCCTAACATAAATGCTTGCATTCTGATTTCAT	218	0.1029336078229542	No Hit
TTATGATGATTCCATGCAATTCCATTAGATGATGACTCCTTTCATTTCCAT	217	0.10246143531000486	No Hit
AGTATACTGTAACAGTTGTAAAAGATCAATATCCATCCATCCTAGTTGAAG	214	0.10104491777115686	No Hit
CAGAGTTACTATTGACAGATATGAGTTTAGTGTCATCATGATATCTATTCA	214	0.10104491777115686	No Hit
GGTATAGCATGCCTTAAATGAACGATGGTTAAAAGTATTCAACTTAAGTTG	212	0.1001005727452582	No Hit
ATCCATATTCTCATCACTAAGATCATCCCATATGGGTTTTATTGAATCCAG	212	0.1001005727452582	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGAGG	20	7.0153386E-4	45.00638	35
CAGCGAA	25	3.876748E-5	45.00638	33
TTGGCAC	20	7.0153386E-4	45.00638	25
ATCTCCC	45	3.8198777E-10	45.00638	26
GCGAATG	25	3.876748E-5	45.00638	35
CCCTATT	45	3.8198777E-10	45.00638	30
TCCGATC	20	7.0153386E-4	45.00638	27
ACCTGTG	25	3.876748E-5	45.00638	35
TCCCTAT	45	3.8198777E-10	45.00638	29
AGTGGGA	20	7.0153386E-4	45.00638	27
GAATACG	35	1.2043165E-7	45.00638	27
TATGCGT	20	7.0153386E-4	45.00638	32
TGCGCTT	20	7.0153386E-4	45.00638	33
ACAGCGA	25	3.876748E-5	45.00638	32
CACAGCG	25	3.876748E-5	45.00638	31
AGCTGGG	35	1.2043165E-7	45.00638	26
GTGAGTC	20	7.0153386E-4	45.00638	26
CTGGGGT	20	7.0153386E-4	45.00638	34
CGACTAC	20	7.0153386E-4	45.00638	35
GCGCTTT	20	7.0153386E-4	45.00638	34

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content