FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_42.35100000043089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_42.35100000043089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192126
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2013910.482183567034134RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT64913.378512018154753No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG11860.6173032280898993No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3080.16031146226955226No Hit
ATCTAATGGAATTGCATGGAATCATCATAAAATGGAATCGAATGGAATCAA2890.15042211881785914No Hit
TTTGAGTGTTTTTCTGTGTTTCAGTTTCCTTCCATTTAGCTCTGATTTTGA2690.1400122836055505No Hit
ATACTATGCAGTGTTTGTTACTTCCTATCTCAAATCCACAGTACATATGGT2670.13897130008431968No Hit
CAGTGATTCCATTCAAGTCCATTTGATGATTGCTTTTCATTCCATTCAATG2360.12283605550524135No Hit
GACAGAAGCATTCTCAGAAACTTGTTTGTGATGTGTGTATTCAACTAACAG2280.11867212142031792No Hit
CTGATTGAATCAATATGTCGAATCAAACAAATTCACAACAACATGTTGAAG2250.11711064613847164No Hit
GTAATAGAGTGTGATGTTGTTAAAAACTATGCCTTTGTTCATATGCTGTCT2240.11659015437785619No Hit
AAGTTATCGATCATCATAACTTTGATGAAAGTGATAGAGGATCGCATACAA2190.11398769557477906No Hit
TCCTGTTACTAAGATGTCTTAAGTGGAATAGAGTCATTCCAAGAACTACAA2140.1113852367717019No Hit
TCATTACCTCTATTCTTACATTAGTTCCACCAATACTGTCTCTTATACACA2130.11086474501108648No Hit
AATCAAAACAGTTTTCCTTAGTGACCATGTATAATCCACGTATAATCCACA2070.1077417944473939No Hit
CATCTGAGTAGTGTAGTCTACTTGAAATTTATGTTCCACTAAACTCCTGTC2010.10461884388370131No Hit
ATTCACTATAGTGCAACAGTTATGACTTGCAAAGTTTGTGTATGAATGTGT1980.10305736860185502No Hit
ACAAAGAACTAATCTTGCCAATGTTGTGTTGTTGTTGAAAAGTCTTGGAAT1970.1025368768412396No Hit
GTGTATAAGCATTGTTAAGGTTTCTAACATTTGCAGAATTTCCACATTTGC1970.1025368768412396No Hit
CAAACTATCTTTCAATACGATAACGGCAATACCACTTATGTGTATCCTTAT1970.1025368768412396No Hit
GTATTATTACGTATGTGCTTGTGACGTAACCAAATATCTGTCTCTTATACA1940.10097540155939332No Hit
AAGGGATACTCAATGCCGACCATTCTTTTTCGTGTGTAAAAGGAATACTGG1930.10045490979877789No Hit
ACTGATTACATCATTTATGTTTTTCAAGGTAACTTCTGTCTCTTATACACA1930.10045490979877789No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGAC207.0098083E-445.0114561
GTCTAGG207.0098083E-445.0114561
GTATGAG207.0098083E-445.0114561
TCACTCG207.0098083E-445.0114561
TAAACGG351.204935E-744.999743
AAACGGC351.204935E-744.999744
TCCACGT351.204935E-744.9997435
ACGTATA351.204935E-744.9997438
CCCTGCA207.018808E-444.99973712
GTCATAA207.018808E-444.99973734
AACGTGG207.018808E-444.99973723
TGTCAGT207.018808E-444.99973743
TAGAGTG302.1561245E-644.9997375
CTAGAGA207.018808E-444.9997379
TTAGGGG207.018808E-444.9997374
CAGCGAT207.018808E-444.99973722
CTGTCCT207.018808E-444.99973721
GTCGCGG207.018808E-444.99973732
ACCGGGA207.018808E-444.99973717
CGAACGA302.1561245E-644.99973740
Produced by FastQC (version 0.11.2)