FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_40.35100000043039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_40.35100000043039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165070
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC49042.9708608469134306No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT12910.782092445629127No Hit
GAATGGAATGGAGTGAAATAAACTCGAGTGGAATGGAATGGAATGGAATAG4630.2804870660931726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG3030.1835584903374326No Hit
ATATACAACGACCACAGCAGTAAATGGAGATAGTTACTCTGTTGAAATGAC2470.1496334888229236No Hit
GAGTACAATTAAGTTCAGTGTTACATCAGAGCCTACGATAAAATCGGCAAC2370.14357545283818987No Hit
GCTTCAATCAATTGTTTCAGTTCTTCTTCTTTTGGCAAATACAGACGGTAT2230.1350942024595626No Hit
ATGATGGGAGTATTTGCATAATGAAAAATGGCAAATGCCACAAATCAGGAA1780.10783304052826073No Hit
TTCTGGAATAGAATGCAGAACCTATAGTGCACAATTAAAGATTCCTGAAGG1700.10298661174047373No Hit
ATAGATGGATCAGCAATACCTGGAGAAACAAATAGTACCTATACAACCACA1670.1011692009450536No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCG207.010831E-445.00636333
GCGGTTC207.010831E-445.00636328
AGCCCTC207.010831E-445.00636323
ACCGAAA207.010831E-445.00636320
CGTAGAA207.010831E-445.00636327
GCCCTCT207.010831E-445.00636324
CGGGATA207.010831E-445.00636340
GCCTACG253.8730133E-545.00636331
CTCGAGT502.1827873E-1145.00636323
GATTCGT207.010831E-445.00636341
ACCCCTC207.010831E-445.00636332
GCCGTTC207.010831E-445.00636317
ACCTTGG253.8730133E-545.00636327
CAGACGG453.8016879E-1045.00636342
AAGTCCC207.010831E-445.00636332
CTATGCT207.021309E-444.9927310
AACCGGG207.021309E-444.992736
CCGGGTG207.021309E-444.992738
ACCGGGT207.021309E-444.992737
GGCACTC207.021309E-444.992738