Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pcontrolb_4.35100000042bc4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 75967 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 2779 | 3.6581673621440887 | RNA PCR Primer, Index 27 (95% over 21bp) |
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 218 | 0.28696670922900736 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT | 209 | 0.27511945976542446 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 191 | 0.2514249608382587 | No Hit |
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 160 | 0.21061776824147327 | No Hit |
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 155 | 0.2040359629839272 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 149 | 0.196137796674872 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 140 | 0.1842905472112891 | No Hit |
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 129 | 0.16981057564468782 | No Hit |
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA | 119 | 0.15664696512959575 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 115 | 0.15138152092355892 | No Hit |
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 114 | 0.15006515987204969 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 110 | 0.14479971566601288 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 103 | 0.13558518830544844 | No Hit |
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA | 92 | 0.12110521673884714 | No Hit |
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 91 | 0.11978885568733792 | No Hit |
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA | 81 | 0.10662524517224585 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 415 | 0.0 | 39.578312 | 45 |
ATTGCTA | 420 | 0.0 | 38.57143 | 31 |
GAATTGC | 415 | 0.0 | 38.493973 | 29 |
CCCACGA | 430 | 0.0 | 38.197674 | 19 |
TTGCTAT | 425 | 0.0 | 38.117645 | 32 |
TGCTATC | 425 | 0.0 | 38.117645 | 33 |
GCCCACG | 435 | 0.0 | 37.75862 | 18 |
AATTGCT | 435 | 0.0 | 37.75862 | 30 |
ATGCCGT | 435 | 0.0 | 37.75862 | 44 |
CGAGACG | 430 | 0.0 | 37.67442 | 23 |
GACGAAT | 430 | 0.0 | 37.67442 | 26 |
ACGAATT | 430 | 0.0 | 37.67442 | 27 |
TATCTCG | 425 | 0.0 | 37.588234 | 36 |
GTATGCC | 425 | 0.0 | 37.588234 | 42 |
CGAATTG | 425 | 0.0 | 37.588234 | 28 |
TCGTATG | 425 | 0.0 | 37.588234 | 40 |
CTATCTC | 440 | 0.0 | 37.329544 | 35 |
CACGAGA | 440 | 0.0 | 37.329544 | 21 |
TATGCCG | 440 | 0.0 | 37.329544 | 43 |
ATCTCGT | 430 | 0.0 | 37.15116 | 37 |