FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_39.3510000004302c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_39.3510000004302c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147936
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC41862.8296019900497513No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT7290.492780661907852No Hit
GAATGGAATGGAATGGAATCAACTCGATTTCAATGGAATGAAATGGAATAG5340.3609669046073978No Hit
AGTCATTAAAACATCTGCTTTTTCTCCAGTTAGATCTATACAACTGGATGG4930.3332522171749946No Hit
ATTCCAAAAGTTCAATTTCACAATCAAGATTTAGTACGTACTGAAAAGGAA4830.3264925373134328No Hit
GATACAAAAGAATAGGCATAAAGCGTCTTCTGTGAAAATCATGCATGTATA4330.29269413800562405No Hit
AAGTGTTTCTGTAACAAATTTGAAAACGAAGAAAGGAACACAAACAGATTC4300.2906662340471555No Hit
ATATACAGCAATCAATGTAATGTACCACATTAACGAAGAAAAGGCAAAAAA4290.28999026606099937No Hit
TCACAATCTAAGTCTCAGTTCCTCTGTGAAACTTCTCTTGTTATACAATGA4090.27647090633787585No Hit
CTATTGAGATAATCATGTGGTTTTTGTCATTAGTTCTGTTTATGTGATGAA4040.27309106640709496No Hit
CTTCCTAATTCACACAAACTTAAACTCTAGTGTTATTTCCTGAACTTAAGA3850.2602476746701276No Hit
ATGTAAATGGATTGGAAGAAGGTAAATGGAAAAATTACTGGAGCAATGGTA3810.2575438027255029No Hit
ATATGCATCACTATAACTAGTACTCCATCCGTCAATTTATAAGTTGTGCTT3690.24943218689162883No Hit
CTTGTATGATCATTATTGGTTTTGGAGTAAGGAAAAAGAAAAGAAACGCAA3630.24537637897469175No Hit
GTGTTAGTTAAGACACAATTTACGATAAACCGAAATACGTACACTCAGTTA3600.24334847501622323No Hit
TTACTATTCCATTGCAATCAACCATAGAAAGAATGAGCTTGAGCAGAAGAT3520.23794073112697384No Hit
AAATATTCTACGTGCTAGATTTAACTGTTGTTTCCTTATGGCATCATTGAA3480.23523685918234913No Hit
AGATATTGGGATATGATATTTGATCCTCAGAAGTCTTCTACTTTCGTAAAA3420.23118105126541205No Hit
AGTGGATACAACTACTTCTGTAAGATTATTGGTAGAAGGAGTTAAGCTTAA3420.23118105126541205No Hit
GAATTATGTCAAGAGATTTCGATATCGAAACAATTTACGTAGACGGAATTT3230.21833765952844475No Hit
GTCCTAATTCAATGATACGTGTAGCAATGGTATTGATGAATTGGTAATCAT3090.20887410772225828No Hit
TACTTGTACAGTACTTTCTTGGTTAAAGAAGTGAGACTTCATGTGATAATT3090.20887410772225828No Hit
ATTGAATTAGTTTCATCGATTTGGTTTGGGTTTTGTTCATCGTACGGAATA3090.20887410772225828No Hit
GGCATTTTTAATGTTCTTAATCGTTGTACTTGGAGATATTGTTGTTCGTAT3060.20684620376378976No Hit
TATCCCACCTTATACACCAGAAATGAACCCTATTGAACAAGTCTGGAAAGA2980.20143845987454032No Hit
TCGTATACGTGTCAAAATCATTGTTAATGGTATTGTACCCATCGATGATAT2920.1973826519576033No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG2900.19603071598529093No Hit
GGTACCTAGGAATAAACCTAACCAAAGAGGTAAAGGATCTATACCCTAAAC2880.1946787800129786No Hit
ACTCCATATCAACCCTGAAATTAAAACGCTTGAAGATATTGAAACATGGGT2870.1940028120268224No Hit
GTGTAACACTGATGTCGTTTTCAGTATCAAAATAATCCGTACATATGACAC2860.19332684404066622No Hit
AAATGGAATCGAATGGAATCACCGAATAGAATCGAATGGAACAATCATCGA2860.19332684404066622No Hit
CAATATAACTGTTGAAACAAAGCTAACATGTACATATCCAAGAAAAATTTC2830.19129894008219772No Hit
ATTAAATTGTTCTAACATCCATTCCATACGTTTGACACCTGGTTTAATACC2820.1906229720960415No Hit
TATCCAAAAGAAACAAGAGCAGTTTTTAACTCATTCTTATCAATGGTCCCA2810.18994700410988535No Hit
AAGGAATCACAATTATACCTTTGCAGATGCTACAAAAATACTGTTACCAAA2780.18791910015141683No Hit
ATACATATCTGAGACAATCAGAAATTTGACGTGAACCTACAATTGATTTAC2770.18724313216526067No Hit
TGATTGTATTGCCTTTCTGATCTACTACTGTTACATGAAGGTCTTCTTCAA2750.1858911961929483No Hit
ATGAAATGCCTTCATTTTTGCATTGAGTTTAACAACATTGGATTCACTTGC2730.18453926022063596No Hit
TACTCATAAAGATAACTAGCCCTTGCATTTCACTTGGAATAGCTTAAACAC2710.1831873242483236No Hit
GATAACAACACATGAACAAAACAAGGAAAGGAGTTTTTTGTAAAAAAACAC2680.18115942028985507No Hit
TTCTTACTCATTTGTGGAAGCAAAAAAGGTGATTTCCTAGAAGTATAAAGG2670.1804834523036989No Hit
ATTATCAACTTGGTTGTTAGTGAAGGATTGTCAAAGTATTCCTCAAATGAC2660.17980748431754273No Hit
AATCATCATCGAATGAAATCAAATGGAATGATCATATGGACTGGAATGGAA2640.17845554834523036No Hit
AAACAACTCTAGAGCAAAGAAATTGGAGTCAATTCAATCAAATACGATGTT2640.17845554834523036No Hit
TGATAAAGAAGAAGGTATTGAAGTGATATTGTTGGAATGGGATGATTTTAT2630.17777958035907418No Hit
GTCTCATTAGGTAAAAATGAGATCTCAGTGAAATAGTCGCATTTTGCAAAC2600.17575167640060568No Hit
GGTTGTAATAGCTTCATCATAAGCATTGGTATGCAAACTGTTACAGTTGTC2600.17575167640060568No Hit
GAGTAGATGTATGAAACTAGTTTATACCTTTCAGAGATCAAGTACAGTGCT2560.17304780445598097No Hit
AACATAATGTGTAGCTTCTCTATCTTTCTTGCTACATCGTGATATTGTAAG2560.17304780445598097No Hit
CCTGTATATTGCTCATACACCAATCAAATTGTAATCATCTCGTATTCCTAT2550.1723718364698248No Hit
GAATTGAATCGTAGAATATCAAATCGAATTGAAAAGAATTGACTCAAATCG2540.1716958684836686No Hit
CTTTATTGCAGAAACTCTTACTCTTGTTCAGCTATTCCTTTTCTTTTCCAA2490.16831602855288774No Hit
TATGAGAACAAATGACTTACACCTCTGATTAGTCCCAAATTTATCTTTGTT2430.1642602206359507No Hit
CTCTTTATCTTGCATGAAACTTAAGATAGATTCACTTGCTTCTTTAGCAGT2410.16290828466363835No Hit
TAACAAAGATTAATCAATGTAATCCACCACATCAATACACTAACTGTCTCT2400.16223231667748217No Hit
AAGAGAAGTATAATGGTGTTAAATCACGAATCGAACAATTAAAAGCATAGG2370.16020441271901362No Hit
AACATACATCCTGCCTTTTCTGTATGAACTGTACCTTGGGTAACTAACTGA2370.16020441271901362No Hit
CTTCTGGTTACCATATTGGGTTGTTTTCTTGTAGGCATACATCTTAAGGCA2320.15682457278823275No Hit
GTTCTTTATCTGTGTTATCAGACTGTTCTTTATCTGTGTTATCAGACTGTT2320.15682457278823275No Hit
TCCAATTACTAGACTTTCGTGGGATAACTATCTAACAGTTTCTCCGAAAGA2310.15614860480207657No Hit
GATCAGGAATAGATGCTGTATTTTGTCAAATGCTTTCTCTGCATCTAATGA2310.15614860480207657No Hit
CATAAATACTCACTGAATTAACGGAAGAATCAAACATCAAATGAATGAAAT2300.1554726368159204No Hit
TAACAGGATTGTCTCCCTACTTCATAAAATTGTTTGCTTTTCCTCTTGTAA2290.15479666882976423No Hit
ATTCGATGATGATTCCATTCGATTCCATTCGATGATGATTGGATTCGATTC2220.150064892926671No Hit
ATCTTCATGTTGGGATGTATCAACATTTTCATCATCTTGTTGAATGTTGTT2220.150064892926671No Hit
TTATTGTTCGCTAGAATTATCCGTTGTCCGTGAATCTGAATGAAGCATAAA2210.1493889249405148No Hit
TAACAAATACCGTGTAAAATTATTCCTGCATATAACATCCAGCTACTGTTT2210.1493889249405148No Hit
TTCCAGTGTTGATGTTATTGCATTAGTTAGTTGTTTATGTGTCTCTCCCTT2170.14668505299589013No Hit
AACTTGGTCTATACTAACTTTGGCATAGACACACTATAACACAAGCATAGA2160.14600908500973395No Hit
TCATTAATGATTGTCATTTTCCTCACCTACAATTGAATGTATTTCGTTTAA2160.14600908500973395No Hit
AGTATGTACTGAACCTTGTCTACATGGAAGATAAGATCAAGTTCACAAACA2150.14533311702357776No Hit
TGGGATAATATTCAGCCATAATAAAGGGAAAATGTCTTTTGCAGCAACATG2090.1412773091066407No Hit
GATTGAATTCGATTCTTTTCGATTCGATTCAATTAGATTCTTTTCGATTGG2090.1412773091066407No Hit
TTTCATAATTGCTTGTCTCTGATGTTAGAGGAAAAACTTTCAGAGGAAAAG2080.14060134112048453No Hit
ATGATTCCATTCGAGTCCATTTGATGATTCCATTGGACTCCATTTGATGAT2080.14060134112048453No Hit
GAAAAGAATCGAATCGAATCGTATTGAATCGAATCGAGTAGAAAAGAGTTG2070.13992537313432835No Hit
TTTCTAATCATTCTGCTAGGCATATTTTACCAAGACATGTAGATTGATGAT2070.13992537313432835No Hit
TATCATTGCACTCTATACCATGATTTAGTGGTCCATTTTGACACTTTTTAA2040.13789746917585985No Hit
GATCATACTATGCGTATTTTCTGACCACAACACTATATTTGAAGTCAACCA2030.13722150118970364No Hit
TGTTAAATGTCACTGGTTATTTTGTAATCCTAGGTAACTCATTATACCTGT2030.13722150118970364No Hit
ATCCATACCTGTTAAGGTTACGTTTGCAACGGTTGAATAATTGTTAACAAT2030.13722150118970364No Hit
GTCATCATCTAATGGAATCGCATGGAATCATCATCAAATGGAATCGAATGG2020.13654553320354748No Hit
CATCAAATCCAAGCTTCTTAGCAAATATGAGCATATCCTTCATCAATTCTT2020.13654553320354748No Hit
CATATACAAGTGTATTGCATATGCATTCATATGAACTTAGTAGTTCCTGTC2020.13654553320354748No Hit
TAAAGAGACTGAAGTATCTTTTCCAGTCACAAGGGAATGCAACAAAAAATC2020.13654553320354748No Hit
TTAGGGAAAAGCATAGAGTCTTTTACGATTACAATACCCGACGGTACTAAA2020.13654553320354748No Hit
GTATTTATCAAATCTGTTCCTATCTAACGGAACGCTATTGGATCAAATGAC1960.13248972528661043No Hit
AGATTTAGCTCTGAAATCAGTATGGTGTTTAGATACAAGCTCTTAATTCTG1960.13248972528661043No Hit
GAAATAAGTTTAATACCTTCTTCAGGAGTGATAGTTACAACAGCTTCTGAC1950.13181375730045425No Hit
AAATGAGGAAATTAAGGTTTAAGCATTTTGCCAAAAGAACAAAATACTATG1940.13113778931429806No Hit
AGTTACATGTTGAAATGGTAATATTTTGGGTGTAGTAGGTTAATAGCTGTC1930.1304618213281419No Hit
ATTCAATTCCATTCGAAGATGATTCCATTCAATTCCATTCGATGATGATTC1920.12978585334198572No Hit
AAGATAAACTCAAAATGGATGAAAGATCTAAATGTGAGACAAGATTCCATC1920.12978585334198572No Hit
GTTTTGTTTCATGTTGCTTCTCTTGTACTTCTTAGCTTTCATGCAAAAAAA1910.12910988535582954No Hit
GAATAAACACCAGTGGAATGAAATCGAATGGAATGAAATGGAATGGAATGG1900.12843391736967336No Hit
TTGTTTTTGACAAGAAATCTATTCCAATAGTTGCCTGATAAGTGTTATCGA1900.12843391736967336No Hit
GGTGAAATAATGGCATTCTCATATGAAGACAAACTAAAAGAATTCAGCTCT1900.12843391736967336No Hit
GATTATTGTTAATGTGTACCTCTGCTGTGTTTGTAATATCTTCTGTCGGAG1890.1277579493835172No Hit
ATTCATACCAGAGAGAAAAAAGTTATGAGAAAAGATGAAAGGATCATTGGT1880.12708198139736102No Hit
ATGAATTGATGGGGTATAAACTGTATTTTGGTGCCTAAAGAAATAAAAGCA1870.12640601341120486No Hit
TAACAATACACTGAACTTATCATTTGCCATTAACCTAGATGCTAACCTGTT1850.1250540774388925No Hit
AACTACAACTCACATTATACTTACTGTTGAGAAATTGGAGAGTTTTTCACT1830.12370214146658015No Hit
GTGACATGCAATGCTAAATGAAAAAACGGATTGTGATCGAGTTGAAATTTT1810.1223502054942678No Hit
AAAGTAAGTAGCGGATGAATGATTAATTCCATTCACATTTACTCGGTGTTA1810.1223502054942678No Hit
ATATTCCCACAGCTCCTTTTGTTTTAACAGACAAGAAAGAGGATATAGCCA1800.12167423750811161No Hit
GACATACAAGTATATGCTTCTGATATTGATTCAGAAATGATTGAAATTGCA1800.12167423750811161No Hit
ATCTATGCCAGAACCTGTTTAGCAACCTATAATCTAAAAGGTGTTCGTATA1800.12167423750811161No Hit
ATAAAACACAATGTCGAGTGAGGGAAATTAACCATCATGGTACACTTAAAA1790.12099826952195543No Hit
GTCAAGAACTGAACACATTGTATTTGCGTTTCTTATAGTCACTTTCTTAGG1790.12099826952195543No Hit
AAATAATTCTGCTCCATTAAAATCAACGATATATTGAACTGACCCACCTGT1790.12099826952195543No Hit
ATGTACATGGATTATTCTTCTTGCATATCAAGACAACAAAAGCACTACAAT1780.12032230153579927No Hit
AACATAATCAGGTTATCGCCAATTACTTCTGTCATGAATTTTTTTACTCCT1770.11964633354964307No Hit
GTTCCGAAATAGTCCATTATGCCTTATCAAAATAACTTCTAACTTTGTGTA1770.11964633354964307No Hit
ATACAGATCATGCATACATTAGAAAGAACGCAACGTTTACCAATTACTATC1760.11897036556348692No Hit
ATCTCACATAGTTACCTTTCTTTCTTTTTTTTGGTGAGAGTACCTAAAATC1750.11829439757733073No Hit
TTATTATGTTATGTTCCTTGTATACCCAGTCTGTTAAGAGTTTTCATCCTG1740.11761842959117456No Hit
ATATGTAATAACACCCTCATTACATGATTTCCTCTAGGTTGAAGTGACTCT1740.11761842959117456No Hit
GTAAGCTTATAGTTGAACTCAATATTAACCATCAATCAAGTGGATCTAATT1720.11626649361886221No Hit
ATAGATAGGATTGGTAGGGTTAAACTGTAAAGCAGCACCTAACAAACTACC1710.11559052563270603No Hit
ATGAATGAGTTGAAAAAATGAAAACATCTTAAGAGTCCATTAACTGTCTCT1700.11491455764654987No Hit
GATTCGAATGGAATCATCGAGTAAAATTGATTGAAATCATCATCAAATGGA1690.11423858966039369No Hit
ATTGACACACACAATAGGGATGATTCACAATGTGTTAAGTAAAGAAATCAA1680.11356262167423752No Hit
AAACTCTACAACAGCTTTGAGGTCATAACTTACATAAAGCTGAAAAGGTTG1680.11356262167423752No Hit
ATTTTACACAACAGGTTTTTGGAATTAAAGTACCACACGATATCTATGTTC1670.11288665368808133No Hit
ATATACAACAGCTTGGAAAACAACATTTAGAAAACAAAAGCCAATGTAAAA1660.11221068570192516No Hit
AGCATCTTATTTGCGAAGATATTGCAACCCAAAAGTTGTTTCATGTTTCAC1660.11221068570192516No Hit
GATCCATACCAGTAAACTTTGATGGCATTCCATTATGTGTTATTGTTAAGC1650.11153471771576898No Hit
CATGAAAATAGAGATCAAAGAATGGTGTTTTAGAACTTCACCAGACCTTTA1650.11153471771576898No Hit
GTATTGAATGAAATTGTCATCAAACGGAATTCAAAGGAATCATCATCAAAT1650.11153471771576898No Hit
TTTCATTTGTTGTCATACAGATAATCACTGGGTTTAGAAATCAGGTATCAC1650.11153471771576898No Hit
TCATAGTTGAAAACGATGTTCACTCTTTCAATATCCATACCTCGCCCAAAT1650.11153471771576898No Hit
CAATTGATCAATCTAAAACAAATCAAAACAACCAACATTCTGTAGCTGAAA1640.1108587497296128No Hit
AATTTGAACGAGCTATACAAATTGTTAGAAACGACAAAATAAAATCACAAT1630.11018278174345664No Hit
ATATAAAGCTTGATATCCATAGAATGTTTCAAGAAGATTGCCTCTTGATAT1610.10883084577114428No Hit
TTGCTAATTCTTTAAGAGTATCCTTTGTACCTTTTCTTCTTTTCCATTCCT1610.10883084577114428No Hit
ACCATATGTGTTTTATTGATGAGTTTGTGAACGATAAAAATCAGCACCATG1610.10883084577114428No Hit
GTTTCTGGGTCATATTACCATATGTTAACTCTAGGTTTAACATTTTGAGCA1610.10883084577114428No Hit
CTTAATGTACTGTTGTATCCTATTGGCTAGTATATTGTTGAGAAATTTTGC1600.1081548777849881No Hit
GTAGTAACTCATGTTTAGCTCCCCTTCCATTCTAAGGTTTGGAATTTAGCT1580.10680294181267574No Hit
CAATAAAGGATACTGAAGATAAAGATTGTGCCACTAACAAAATCCAATATC1580.10680294181267574No Hit
TCGTAAAGTATAAATACCTTCAAACTAAACCAAGACATGCATTATAGCTGT1580.10680294181267574No Hit
TATATCCTCAGGGAGATGACTAATTAACCACAAAAGGACCAAAGGAAAACA1570.10612697382651959No Hit
AGTATAATCTATTGCCAACTTGTTTAAGTTCAGGAGTTATCACATCAGCTA1560.10545100584036339No Hit
CCTATTGATCTAACTGTTAATGATTTAGGACAAAGTTTACGGAACGATTAT1560.10545100584036339No Hit
AAATTCTCTCATTTGAAGAACATTACAAGAAACTGGGAATATTTAAATCCC1550.10477503785420723No Hit
ATATATCGAAGTGATGATTTGTTGGCGTAATAACATCAATAGCGTCACAGG1550.10477503785420723No Hit
CAAAGAATTTGAAAGACGATGATGATTGTAAAGATCGAGTGAAGAACATGC1550.10477503785420723No Hit
GTATACGGATTCAAATACATCCAACTAAACATTTGAAAATTGAGCCATGGA1550.10477503785420723No Hit
GATTTAGATAAGCTTTTAGAGCTTAAAGATGTTGCATTTGTTGAAAAAGAC1550.10477503785420723No Hit
GAGTAGAGTAGACTACTACTCTACTCTAGTAGTAGAGTAGTACTACTACTA1540.10409906986805105No Hit
TGTTGGAACAATGATAGAATTCTGGAGTTTTCGAATTACCAATTAAACTTA1540.10409906986805105No Hit
ATTATATGGACCTAAAGCTGCCTTCAACTTTGGTCACTTTGTAGAACCTAA1520.1027471338957387No Hit
CAGGTCTTATATATCAACCTTTGACCCATCTTGAGTTGATTTTTGTATAAC1520.1027471338957387No Hit
ATCTTCTTGTCTTCTGATGTCGCTTTAACTTTGGAGAAACTTCTGATGATA1520.1027471338957387No Hit
TATTATCATACTGTGCAAAATTGGAAACCGAAACATATACTTTATTTGCAT1500.10139519792342634No Hit
GACTTAACAATATTCCAGTTTGAGAACGGATAGAGTTGAGTGTATAATTAC1490.10071922993727016No Hit
TGTTAGAAGATAAAACGAGAGTATTACAGATGTTAAATACACCAATCGAAG1480.100043261951114No Hit
AGTCAAGAGAATGTGATTTTGTTTTGTAACTTTGTTTCAGAATCCCAATTC1480.100043261951114No Hit
AGGTTAGACCTAAATTTGTCCAATTTATGATGCTTCTAGGGAAAAGATGGA1480.100043261951114No Hit
GATCTTATTTATGCCTAGAAAATCCTAAAGACTCCTACAAAAGACTCCTAA1480.100043261951114No Hit
CTGTATAACTTTCAATTGTTTTTGCCAATCTTTTTCTTGTTTAACCTGATC1480.100043261951114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT302.1488613E-645.011525
ACTGACG302.1488613E-645.011528
ACCGAAA750.045.011529
CTGACGA302.1488613E-645.011529
CTAGTGT650.045.011527
ATTGACC302.1488613E-645.011527
AGTACGT750.045.011533
TAGTCGC302.1488613E-645.011534
AGTCGCA302.1488613E-645.011535
AACGTTG207.004516E-445.01149733
GAGTGAT207.004516E-445.01149726
AATCCGT351.1999327E-745.01149734
GGTGTAT207.004516E-445.01149732
CAACGTT207.004516E-445.01149731
AAGAGGT351.1999327E-745.01149725
GAATGGG253.8684397E-545.01149735
ACGCACT207.004516E-445.01149735
GCAACGT207.004516E-445.01149730
CCGTGTT253.8684397E-545.01149729
CCTACTG207.004516E-445.01149732
Produced by FastQC (version 0.11.2)