FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_38.35100000042ff7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_38.35100000042ff7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233125
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC131575.643753351206434No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT21370.9166756032171581No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG6530.2801072386058981No Hit
GAAGCATTTGTTGTACTTGAGACTTCAATTCACGTGGAGAATCAACTTCAC3400.14584450402144772No Hit
AATCAATATCACGTCACAGGTGTTGTTTGATTACATTTTACTGATCCCATC2720.11667560321715817No Hit
AACACAAAGAGTTTTGAAAACATTTCGAAGTGGTTAAGAAACATTGAAGAG2510.10766756032171582No Hit
CTGAAGATATGCTAAACATAACAACTCAATAGGTACTAATTTTGGCATCAG2480.10638069705093833No Hit
GCTATAGTCTTCTAACAACAAACAACGATTTGTTTCACTATCGATAACACA2450.10509383378016085No Hit
TAACATTTGTTCCCGCACAAATTGTAGTACTTGTAGAAGTAATTGAAACAC2430.10423592493297587No Hit
AGACCAAATTGGAAAACAGCAAATGATCTCATGAACAGGTTCAACAATGAA2420.10380697050938338No Hit
ATACAAGACAGAATGTGACTCACTTATTGATCAGTACGGGGTTGTTATCAT2340.10037533512064344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG351.2050077E-745.0064433
TGGAACG351.2050077E-745.006442
ACGTGGA351.2050077E-745.0064432
GGAACGG351.2050077E-745.006443
AACCGAG207.016758E-445.00643531
GGTAACG253.877931E-545.0064351
CGGTTAT207.016758E-445.00643534
AGGGTGC207.016758E-445.00643535
CGCCTTA302.155908E-645.00643528
GGCTTGC302.155908E-645.00643532
GAGCGGT207.016758E-445.00643531
CCGAGTG207.016758E-445.00643533
ACCAGTC253.877931E-545.00643528
CTCGACT302.155908E-645.00643534
GCGGAGC207.016758E-445.00643531
GGTCTTA207.016758E-445.0064351
TGGCTAA253.877931E-545.00643533
CCGTTTA253.877931E-545.00643526
CAGAGCG207.016758E-445.00643529
CGGAGCA207.016758E-445.00643532