FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_37.35100000042fdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_37.35100000042fdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146783
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC146179.958237670574931No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT112787.683451080847236No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG16571.1288773223057167No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6030.41081051620419257No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5000.34063890232520116No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2120.1444308945858853No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1880.12808022727427562No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1630.11104828215801557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15700.044.2834445
TTGGCCT250.002101136836.00000425
GGATCTA250.002101136836.00000425
CAGATTA250.002101136836.00000424
TACTATG250.002101136836.0000047
TGAATAC250.002101136836.0000043
CGGGTAT401.5513442E-533.7516
TTAAATC352.8084812E-432.1428573
TTAACAC501.9660893E-631.5000023
CTCTGCA501.9660893E-631.5000029
GCAAGAC300.00513280930.0000023
ACACCTC300.00513280930.00000229
GGATAAC300.00513280930.00000211
CCGTCTG300.00513280930.00000210
GGCTTTA300.00513280930.00000243
TCCAAGG300.00513280930.00000220
TGAGGTT300.00513280930.00000211
TGAGGCC300.00513280930.0000023
GGACACC300.00513280930.00000227
ACGGGTA507.11665E-527.00000215
Produced by FastQC (version 0.11.2)