FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_36.35100000042fb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_36.35100000042fb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260013
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC45791.761065792864203No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT5970.2296039044201636No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT5350.20575894282208965No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAA5180.19922080819035973No Hit
GAGTAGTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGC3490.13422405802786783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCC453.8198777E-1044.99500338
CCGGGAT207.027055E-444.99537
ACGCAGG302.1606538E-644.99544
CACTAGG253.8850012E-544.99519
CGCAGGA253.8850012E-544.99545
CTACCGG207.027055E-444.99534
CCCGCGG207.027055E-444.9959
CCCGATT207.027055E-444.99535
TGCCGTC4500.043.49516745
AGCCCTC1050.040.70976316
CAGCCCT1050.040.70976315
TCAGCCC1050.040.70976314
CCCCGAT501.0750227E-940.495541
GTCTACC356.2261806E-638.57456224
CTATTAG301.1330915E-437.52471
GAACCGA301.1343763E-437.517486
ACGTTGG301.1382379E-437.49583444
GTCCCCG552.733941E-936.8140939
TCCCCGA552.733941E-936.8140940
CCCGATC552.733941E-936.8140942
Produced by FastQC (version 0.11.2)