FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_35.35100000042fa7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_35.35100000042fa7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314406
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC111263.5387365381067792No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT18650.5931820639555224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG5410.1720705075602883No Hit
AGTCTATATAGTCCACATTGATGACCACTATTGTTGGACCAAGTCCTCTCC3540.11259327112078014No Hit
GGTTTATTATTCCCCCTGAAAATCAGAGCACTTAACAGAATTACACAAATC3270.10400564874716131No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT3160.10050698778013142No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACA253.8812403E-545.0060425
GCCCATA253.8848888E-544.99888636
CTATCGG207.026318E-444.99888641
CAGCCAC253.8848888E-544.99888621
GTGGGGA406.7884685E-944.99888628
GACGTCC253.8848888E-544.99888625
GGGAGTC253.8848888E-544.99888643
CACGAGC207.026318E-444.99888639
GTGTGGG207.026318E-444.99888639
GGGGTAC207.026318E-444.9988867
CTACGCA207.026318E-444.99888623
ACTACGC207.026318E-444.99888622
CTTGACG253.8848888E-544.9988869
TAGGGTC207.026318E-444.99888613
ACGTCCG253.8848888E-544.99888626
CGTAAAT207.026318E-444.99888641
GGGTTAG207.026318E-444.99888634
GGCATAC207.026318E-444.99888610
CTGGGGT207.026318E-444.99888622
ATCGGCA207.026318E-444.9988867
Produced by FastQC (version 0.11.2)