FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_31.35100000042f13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_31.35100000042f13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC58.19672131147541No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAACAACC11.639344262295082Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCAGAGCCCACGAGACCTGTCTGCATCTCGTATGCCGTC11.639344262295082TruSeq Adapter, Index 18 (95% over 21bp)
CCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAAAGAACCAA11.639344262295082No Hit
GTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGCC11.639344262295082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTAA11.639344262295082No Hit
GCGTGAAGAACTGCCCGAGCTGCTGCTTACCACCTCGGCTCGAAAGCTCCT11.639344262295082No Hit
GTGTCTGAGGAGTATTTAGCCTTACCAGATGGTGCTGGCAACTTTATGCAA11.639344262295082No Hit
GATACCACTGCTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC11.639344262295082No Hit
TTTTTTTTCTTCCTCAGAATCATCATCATCATCATCAGATTCTTCATCACT11.639344262295082No Hit
CATCTGGAGATGAGGAAAATGAATAAACGAATTCATTTGCGAAATCAAATC11.639344262295082No Hit
GCACCGTATCGAACATTCAAATTTTTTTTACTGTTTTCACTACTTTTGATA11.639344262295082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCCCCCCC11.639344262295082No Hit
AGATATGAATCAATTGACAGCATTAATGATGGTATCAGAAAAAGGTCATAC11.639344262295082No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACT11.639344262295082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATACCACTT11.639344262295082No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTATAC11.639344262295082No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAAACATCTC11.639344262295082RNA PCR Primer, Index 23 (96% over 28bp)
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTGGTGG11.639344262295082No Hit
TCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAA11.639344262295082No Hit
GAGTACGGGACGTTGAAATACCACCCTGGTGTGTTTGAGGTTCTAACCTAG11.639344262295082No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT11.639344262295082No Hit
CCTTAGTGTGTGATAATTTGCTCAAAGCCTACATATTATCAGCTGTTGGAA11.639344262295082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTCTACCCCCCCACTA11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC11.639344262295082No Hit
TCTTTAAGAAGAACAGATTTTCTACTGGGAAGTTGAATCTTTCTTCTCCTT11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGATAATCTCGTATGCCGTC11.639344262295082No Hit
CTTATAACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT11.639344262295082No Hit
AGTTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTATACACAT11.639344262295082Illumina PCR Primer Index 9 (96% over 28bp)
GTACAAAAATTATTAGTTTCGAGAAAGGGCCTATTAACTGTCTCTTATACA11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGCATAATCTCGTATGCCGTC11.639344262295082No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC11.639344262295082No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAAACGTCC11.639344262295082RNA PCR Primer, Index 23 (96% over 28bp)
CTCCTGCTGCGTGAGGCCTTGCGGTCCCCCACTTTCACCCTCAGGTCGTAT11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGAATCTCGTATGCCGTC11.639344262295082No Hit
ACATTAATTTACGATTGTCTATTTCCATTGTTGCTTAGCAACGACTGCAAA11.639344262295082No Hit
TATCAACGCAGAGTACGGGACGCAGAGTACTTTTTCTGTCTCTTATACACA11.639344262295082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA11.639344262295082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCCATCATCTCGTATGCCGTC11.639344262295082No Hit
GAGTAGCGATAAAGGGGGTGAAAGGCCCCCTCGCCGTAAGCCCAAGGTTTC11.639344262295082No Hit
CTATTCTGTTCCTTAATACATTTCTATAAGGCCTGTCTCTTATACACATCT11.639344262295082No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT11.639344262295082TruSeq Adapter, Index 11 (95% over 23bp)
CAACCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGT11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTGTGCATCTCGTATGCCGTC11.639344262295082No Hit
CTTATACACATCTTCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC11.639344262295082No Hit
GCAATCTGGTTTGCAGTAAAAAAAAGTTCAACATCCACTGCATTTTATCTG11.639344262295082No Hit
TCTTATACACATCTCAGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT11.639344262295082No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATATGAGAAACT11.639344262295082RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTATTCACAATCTCGTATGCCGTC11.639344262295082No Hit
CTTATACACATCTCCGAGCCGACGAGACCTAACTGGATCTCGTATGCCGTC11.639344262295082No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTTTCTCTTAAACACAT11.639344262295082No Hit
GAGTACGGGGTATCAACGCAGAGTACTCTTTTCTGTCTCTTATACACATCT11.639344262295082No Hit
ATAAAATGCCTTGGACTGGGAAATTTAGAAACCAAAGAAATTTATTGCTCA11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGCATCTCGTATGCCGTC11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGAACAGCTCTGGATCCCGTATGCCGT11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTACTGGATCTCGTATGCCGTC11.639344262295082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
GTATGCC50.045.042
AATCTCG50.045.036
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
GCCCACG50.045.018
TATACAC50.045.03
TGCCGTC50.045.045
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCC50.045.09
TGCTGAA50.045.031
GAGCCCA50.045.016
CACGAGA50.045.021
CCTTGCT50.045.028
CCCACGA50.045.019
ACATCTC50.045.08
TTATACA50.045.02
TACACAT50.045.05
Produced by FastQC (version 0.11.2)