H23LNBCXX_l01n01_bir_1228_pcontrolb_30.35100000042f06.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_30.35100000042f06.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	4	8.0	No Hit
GGTATCAACGCAGAGTACGGAGCAGTGGTATCAACGCAAAAACTGTCTCTT	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAACTATCTCGTATGCCGTC	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTAATCTCGTATGCCGTC	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	1	2.0	RNA PCR Primer, Index 46 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGCCGTATCTCGTATGCCGTC	1	2.0	No Hit
AGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTTATAC	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC	1	2.0	RNA PCR Primer, Index 45 (95% over 21bp)
GAGTACGGGGGGGTATCAACGCAAAAAAAAAAAAAACTGTCTCTAATACAC	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGTAATATCTCGTATGCCGTC	1	2.0	RNA PCR Primer, Index 44 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGGTATCAACTGTCTCTTATACACATCTCCG	1	2.0	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG	1	2.0	No Hit
ACGCAGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAT	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCGGAATCTCGTATGCCGTC	1	2.0	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT	1	2.0	No Hit
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTCTTTATAC	1	2.0	RNA PCR Primer, Index 33 (96% over 28bp)
GTATTATAGAAGAGGTTAAGTAAAACATCCTGTAGTCCTGAATTTTAGTTG	1	2.0	No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTCGTAAGCCG	1	2.0	RNA PCR Primer, Index 23 (96% over 28bp)
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATAC	1	2.0	No Hit
ATTCCATTCCATTCCATTCCGGAAGATTCCATTCCATTGCATTCCATTCCA	1	2.0	No Hit
TCGCCTTGGTGGGCCTTTACCCCACCAACTAGCTAATCAGACATCGGCCGC	1	2.0	No Hit
GTCACCAGACGGAGGCGGAGCGAAAGCGAGTCCGAAGGGCGCGAAGTTGCA	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTCTGCCGTC	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCTGGATCTCGTATGCCGTC	1	2.0	No Hit
AATAAAATCCAGGTAAATGTAGCTTACAAGAGATAGCCCTAACACAAAAAC	1	2.0	No Hit
ATCTACTACTTTCATGGTATGATGTTAATTTTCTTCTCAGAGCAAAAATTA	1	2.0	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT	1	2.0	RNA PCR Primer, Index 45 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC	1	2.0	RNA PCR Primer, Index 40 (95% over 21bp)
GAGTGATAAAGTCACCATTTCTTTGTGCTACAATTTGCGGTGCATTTTTGC	1	2.0	No Hit
ATACCCAAATTGATGATATTTTTCCCGGCTTTATTGAGTTGATCAATTTCA	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGCAGAATCTCGTATGCCGTC	1	2.0	No Hit
GTATCAACGCAGAGTACGGGGGGTATCAACGCAAAAAAAAAATAAAAAATA	1	2.0	No Hit
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAGCAGA	1	2.0	RNA PCR Primer, Index 33 (96% over 28bp)
TTTCTAGCCTTTTTGTTTTCTCTTCTTCGTTATCTTCGCTTTTTATTTCTT	1	2.0	No Hit
TCTTATACACATCTCCGAGCCCACGAGCTCCTGGTCATCTCGTATGCCGTC	1	2.0	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATTCTGATT	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTAAACTATCTCGTATGCCGTC	1	2.0	No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCATGGCGACG	1	2.0	RNA PCR Primer, Index 23 (96% over 28bp)
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACAACCTCCT	1	2.0	RNA PCR Primer, Index 23 (96% over 28bp)
GTTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATAGGTTGTTGTTGAT	1	2.0	No Hit
GTGGTTCAACACCGGACTGCACTGGGCCTACGGCGTCGATGGTCCCGGCCA	1	2.0	No Hit
GTCTTGATACTGATTGATCAGACACTGATCATCTCTGTCTGTCTCTTATAC	1	2.0	No Hit
GCCCCGTACTCTGCGTTGATACCACTGCTCCGTACCTGTCTCTTATACACA	1	2.0	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	1	2.0	No Hit
GCCCAACATTGGTTATGGAAGCAACAAAAAAACAAAGCACATGCTGCCCAG	1	2.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTACTGGATCTCGTATGCCGTC	1	2.0	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTGTTTT	5	45.0	42
GTACTTT	5	45.0	8
ACGCAGC	5	45.0	1
TTTTTTG	5	45.0	38
TGTTTTA	5	45.0	43
TTTTTGT	5	45.0	39
GTTTTAA	5	45.0	44
ACTTTTT	5	45.0	10
TTTTAAT	5	45.0	45
GCAGCGT	5	45.0	3
CGTACTT	5	45.0	7
CGCAGCG	5	45.0	2
GCGTACT	5	45.0	6
CAGCGTA	5	45.0	4
AGCGTAC	5	45.0	5
TACTTTT	5	45.0	9
TTTGTTT	5	45.0	41
CTTTTTT	5	45.0	11
TTTTGTT	5	45.0	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content