Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pcontrolb_3.35100000042bab.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 170092 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 7360 | 4.327070056204877 | RNA PCR Primer, Index 27 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT | 590 | 0.3468711050490323 | No Hit |
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 321 | 0.18872139783176164 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 291 | 0.17108388401570915 | No Hit |
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 285 | 0.16755638125249864 | No Hit |
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 266 | 0.1563859558356654 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG | 243 | 0.14286386191002517 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 231 | 0.13580885638360415 | No Hit |
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 226 | 0.1328692707475954 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 222 | 0.13051760223878842 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 200 | 0.11758342544034993 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 182 | 0.10700091715071844 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATAACGC | 35 | 1.1988959E-7 | 45.023247 | 3 |
ATGCCGT | 820 | 0.0 | 40.881207 | 44 |
TAACGCA | 50 | 1.0641088E-9 | 40.52092 | 4 |
TGCCGTC | 830 | 0.0 | 40.38866 | 45 |
TATAACG | 45 | 1.9030267E-8 | 40.02066 | 2 |
GACGAAT | 855 | 0.0 | 38.68143 | 26 |
TCGTATG | 870 | 0.0 | 38.531715 | 40 |
CACGAGA | 880 | 0.0 | 38.349514 | 21 |
CCCACGA | 880 | 0.0 | 38.349514 | 19 |
CGTATGC | 875 | 0.0 | 38.31153 | 41 |
TATGCCG | 875 | 0.0 | 38.31153 | 43 |
CTCGTAT | 880 | 0.0 | 38.09385 | 39 |
CGAGACG | 875 | 0.0 | 38.054405 | 23 |
CTATCTC | 870 | 0.0 | 38.01451 | 35 |
TTGCTAT | 860 | 0.0 | 37.93332 | 32 |
GCCCACG | 890 | 0.0 | 37.918625 | 18 |
GTATGCC | 885 | 0.0 | 37.878635 | 42 |
ACGAGAC | 885 | 0.0 | 37.878635 | 22 |
TATCTCG | 880 | 0.0 | 37.83819 | 36 |
AATTGCT | 875 | 0.0 | 37.797283 | 30 |