FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_27.35100000042ea8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_27.35100000042ea8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC35.454545454545454No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT35.454545454545454No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCCGAATCTCGTATGCCGTC11.8181818181818181No Hit
AAAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCT11.8181818181818181No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.8181818181818181No Hit
TGCTAAACAGATAAGTCACAAATTTGGCTATATACTTACTGTCTCTTATAC11.8181818181818181No Hit
TACTGGAATAAAGTTCAGATTCTAGTCTCTTGCAGTCTGGTCTTGACTTAC11.8181818181818181No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAACGAC11.8181818181818181No Hit
CTTATACACATCTACGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC11.8181818181818181No Hit
GTCCACGATCGTGCCCGCCCGCATCAGCAGGCCCTTCTCGGCCAGGTGTGC11.8181818181818181No Hit
ATCAACGAGAGTACGGGTGGTATCAACGCAGAGTACTCTGTCTCTTATACA11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC11.8181818181818181No Hit
GGCACTATTTTTTGATATTTTTCAATGCATCCCAAATAAACCTGTCTCTTA11.8181818181818181No Hit
CATTGAAGCTGGTGGCAGAGCTTTCCAGCTTTACACATCTGGTGTGTTTAC11.8181818181818181No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT11.8181818181818181No Hit
CTTATACACATCTCCGGCCCAGAGACTCGTGTGCATCTCGTATGCCGTCTT11.8181818181818181RNA PCR Primer, Index 16 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTAGTATGCCGTC11.8181818181818181No Hit
TTACTGATTTATTTCAGTGCACTTGTTGTTCTTCTAGTGGTCTCTCACCTC11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC11.8181818181818181RNA PCR Primer, Index 40 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCACATAGAGAACCCGGAAGCCGTCC11.8181818181818181No Hit
TTAGTGCCTTACCAGATATGAGATTTGCAAATATTTTCTCATTCAGTAGGT11.8181818181818181No Hit
CTTATACACATCTACGAGCCCACGAGGACGTTAGGTCATCTCGTATGCCGT11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCTGGAACCCGTATGCCGTC11.8181818181818181No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATCTGTCACCTATACACATCTCC11.8181818181818181No Hit
CATGCCGGGACGCCGGCACAGCGGCCTGGCGAGGTAGTCCGGAATCATCGC11.8181818181818181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGCAGCATCTCGTATGCCGTC11.8181818181818181No Hit
GCAAGAACAAACCAGGAATCCCAAGAGGACCCACAGCTGTCTCTTATACAC11.8181818181818181No Hit
TATCAACGCAGAGTACGGGCAACGCAGAGTACGGGCAGTGGTATCAACGCC11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAACGTTAGGTCATTCCGTATGCCGTCT11.8181818181818181No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTAAAAAACC11.8181818181818181No Hit
AGATGGTCCTTTACCTCCACGTGTTTGAAATCCTTCCAAACTTCTTGTGAT11.8181818181818181No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATAAAATA11.8181818181818181RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGAGCACACGAGACTCGTGTGCATCTCGTATGCCGTC11.8181818181818181No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTACCGTATAC11.8181818181818181Illumina PCR Primer Index 11 (96% over 30bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC11.8181818181818181No Hit
AAATAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA11.8181818181818181No Hit
CTTTTAACGTGTACGCCTCATTCACATCAATGCTTTTAGTGCTATTTGTTT11.8181818181818181No Hit
TTAGCAACCTCAGCATACAATTTTGTTTCCTTACTAAGTTGAGAAATGTCA11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC11.8181818181818181No Hit
TAGCTAACAAGTGGAGGGGCTAAGACATGAATCTGTCTCTTATACACATCT11.8181818181818181No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAACTTACAGACCG11.8181818181818181RNA PCR Primer, Index 23 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC11.8181818181818181No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGACTCCATAGCCAGACTC11.8181818181818181No Hit
AGGATGCTCTTGGTTGTAAACCTCTTTTTCTGCTGTCTCTTATACACATCT11.8181818181818181No Hit
GGTATCAACGCAGAGTACGGGTGTGGTATCCTGTCTCTTATACACATCTCC11.8181818181818181No Hit
GTTCCCATGAATCTTCTTAATTCTTCTCCAATTTCCTGGTTAAGCCATTCA11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTGCAGGATCTCGTATGCCGTC11.8181818181818181No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8181818181818181No Hit
ACTTCAAATGCATGAAAGAGATTAAACTACTTGATCCAACGCCAATTACAA11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTACTGGATCTCGTATGCCGTC11.8181818181818181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.018
GTATGCC50.045.043
TTGCAGA50.045.031
AATCTCG50.045.037
ATCTCGT50.045.038
ATCTCCG50.045.011
CACATCT50.045.08
GCCCACG50.045.019
TATACAC50.045.04
CCACGAG50.045.021
TATGCCG50.045.044
CATCTCC50.045.010
GAGCCCA50.045.017
CACGAGA50.045.022
CCTTGCA50.045.029
CCCACGA50.045.020
ACATCTC50.045.09
TTATACA50.045.03
TACACAT50.045.06
ACACATC50.045.07