FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_25.35100000042e64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_25.35100000042e64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC35.555555555555555No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCCGAATCTCGTATGCCGTC35.555555555555555RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC23.7037037037037033RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC23.7037037037037033RNA PCR Primer, Index 40 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGTAGGATCTCGTATGCCGTC11.8518518518518516No Hit
GGAGAGAGAGGTCATCCTTCTCCCCCACCATCATTACGTGATCATCCTGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATCACAATCTCGTATGCCGTC11.8518518518518516TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTCTGTCTCTT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTTAATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACGAATCTCGTATGCCGTC11.8518518518518516No Hit
TCTTATACACATCTACGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCAGAATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCTGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC11.8518518518518516RNA PCR Primer, Index 45 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC11.8518518518518516No Hit
GGACAAGTGCACCTAGATAGTTTTTGACCGGGCAGGAATGAAAGCTGTCTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGAGAATCTCGTATGCCGTC11.8518518518518516No Hit
GTATAGATCACAAGGACCTGATCTTTTGGGGAAGACTATTGAAGAGAGAGG11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTACTGTATCTCGTATGCCGTC11.8518518518518516No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGTTGAATCTCGTATGCCGT11.8518518518518516No Hit
CTCTTATACACATCTCAGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTGTAGAATCTCGTATGCCGTC11.8518518518518516No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAGTACAGG11.8518518518518516Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTTTGTGCATCTCGTATGCCGTC11.8518518518518516No Hit
ACATTAATTGTCGGCTTTAATTATAATTATCCCTGTCTCTTATACACATCC11.8518518518518516No Hit
CTTATACACACCGAGCCCACGAGACTCGTGTGCATTCGTATGCCGTCTTCT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGCATCTCGTATGCCGTC11.8518518518518516RNA PCR Primer, Index 43 (95% over 21bp)
CTTTTATCTTTTCTTCTTCATTTTTTGAAGCAACTTCATCTTCAAATTGCT11.8518518518518516No Hit
AGTTATTTACTTCTACAAGGACGTATCTATTGACGTGTTACCCTGTCTCTT11.8518518518518516No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGGATCTCGTATGCCGTC11.8518518518518516No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATAGACGT11.8518518518518516RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCGTGTTCATCTCGTATGCCGTC11.8518518518518516No Hit
GGGTGACAGCCCCGTACACAAAAATGTACATGCTGTGAGCTCGATGAGTAG11.8518518518518516No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATCTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATAGAATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTTCGCTATCTCGTATGCCGTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGTAGGATCTCGTATGCCGTC11.8518518518518516RNA PCR Primer, Index 22 (95% over 21bp)
GCGACCACCGAGATCTACACACAGGTATTCGCTGTCTCTTATACACATCTC11.8518518518518516Illumina Single End PCR Primer 1 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC11.8518518518518516No Hit
TTGATGATTCAAGTTTTGATGAACAACACAGTATCTGTCTCTTATACACAT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTGCAGAATCTCGTATGCCGTC11.8518518518518516No Hit
AACTGGCCTCTTTACTAATTAGTTCTGAAACTTTTTCAATTCCTTTGATTT11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCATCATCTCGTATGCCGTC11.8518518518518516No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC11.8518518518518516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTG50.045.041
AAACTTT50.045.028
ACTAATT50.045.014
GCCTCTT50.045.06
GAAACTT50.045.027
CTTTACT50.045.010
ATTAGTT50.045.018
TTCAATT50.045.035
TTACTAA50.045.012
AATTCCT50.045.038
AACTGGC50.045.01
GTTCTGA50.045.022
CTCTTTA50.045.08
TTTTTCA50.045.032
TTTTCAA50.045.033
AATTAGT50.045.017
TTCTGAA50.045.023
CAATTCC50.045.037
TTTCAAT50.045.034
TCAATTC50.045.036
Produced by FastQC (version 0.11.2)