FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_23.35100000042e21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_23.35100000042e21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60229
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC904515.017682511746832No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT9581.5905958923442196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG3620.6010393664181707No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2640.43832705175247805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.4034601271812582No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2340.38851715950787824No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2280.3785551810589583No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.3636122133855784No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.3337262780388185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.3204436401069252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.31878331036543855No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1840.3055006724335453No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.2988593534675987No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.24904946122299887No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.21916352587623902No Hit
TTTTTCTTACTGATTGAGGATTCCTAATGTGATGAAAGCTAAATTAAAGCT1260.20920154742731906No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1250.2075412176858324No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1200.19923956897839912No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1080.1793156120805592No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.17101396337312588No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.16769330389015258No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.1627123146656926No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC880.14610901725082603No Hit
GATAATGTCATTGAGATGAGATGATGTTGATGTTGAGAATGATATTGAGAT860.1427883577678527No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA850.14112802802636604No Hit
TAGAGAGATAGAGAAAGAGAGAGAAGAGAGACAAATGGAGAAAGAGACAGA830.1378073685433927No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA830.1378073685433927No Hit
TTTTTTTGGTTTACCTTCGTTTAATGCTTGTTGTTTTAATACTTCTCTTTC820.13614703880190604No Hit
GGTTATTGTTATTGTGGTTTTGATTCTGAATCTGTCTACGAATTTGATACA810.1344867090604194No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA800.13282637931893274No Hit
AGATTATTGACTAAAGAGACCTTCACTAAAGAAGAAAACAGTAGGATTAAA800.13282637931893274No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA790.1311660495774461No Hit
GTATAAGACAGACCCAATTTACAGACTGTATGGAAAATTTGAACAAGAAAG780.12950571983595943No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA710.11788341164555281No Hit
ATAAAAGGTTCATGGAAGATTTTGTATCTTGGAATCTCTAAACGCTTTGAT690.11456275216257948No Hit
ATTGATGAAGTTCAAATGATGGATGATTTTGTAGATGTGCTTAATAGTTTT670.11124209267960618No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10792143319663286No Hit
CTATCAAAGAAGGAAGATGGATTATGTACCAAGAAACAAGAATATGATTGA620.10294044397217286No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA610.10128011423068621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGA206.955323E-445.0324173
TAATGTC206.955323E-445.0324173
TATAGGG206.955323E-445.0324172
ATATAGG206.955323E-445.0324171
ACTAAGT206.983899E-444.99501812
TTCATCT206.983899E-444.99501833
CCAACGA253.8492966E-544.99501823
CATTGAG206.983899E-444.9950189
TTACTAA206.983899E-444.99501810
TAAGTAT206.983899E-444.99501814
GTATTTC206.983899E-444.99501817
CGAAGAT206.983899E-444.99501824
TCAATCA206.983899E-444.99501836
GAATGAA206.983899E-444.99501828
TGAAGTC206.983899E-444.99501831
CGAATGA206.983899E-444.99501827
TCCAACG253.8492966E-544.99501822
GATTACT206.983899E-444.9950188
TCATTGA206.983899E-444.9950188
ACGAATG206.983899E-444.99501826
Produced by FastQC (version 0.11.2)