FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_22.35100000042e07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_22.35100000042e07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226168
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC182568.071875773761098No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT47432.097113650030066No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG12340.5456121113508542No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT5590.2471614021435393No Hit
GGTATAACACAGGAACGGAAACATAGTAGAACACTTATTCATCAGGGATTA2440.10788440451345903No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCACT207.022278E-444.99867633
GAGGCAC207.022278E-444.99867632
TGCCGTC18400.044.14271545
TGAGGCA301.137222E-437.49889831
TCCCTGA1050.036.4274987
GACCAGT250.002101560636.00696
CCAGAAC250.002103843535.9989435
CGATCCG250.002103843535.9989416
AATCCCT1100.034.7793925
CCCTGAT1100.034.77178
ATGCCGT23500.034.5628144
TATGCCG24050.033.77239643
GTATGCC24100.033.70232842
CGTATGC24250.033.4938641
TATCTCG24050.033.49173736
CTCGTAT24150.033.4462239
TCTCGTA24150.033.4462238
CACGAGA24300.033.4249421
ACGAGAC24300.033.4249422
CCCACGA24400.033.3801619
Produced by FastQC (version 0.11.2)