FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_21.35100000042dec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_21.35100000042dec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83553
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC2931935.090301964022835No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT1701720.366713343626202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG34004.06927339532991No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3810.4559979893002047No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2770.33152609720776033No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2080.24894378418488863No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1650.19747944418512803No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCC1580.18910152837121347No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA1490.17832992232475195No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1330.1591804004643759No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTAT840.10053498976697425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31350.044.4222945
TCCTATA250.002096789235.99712834
GCCGTCT352.799505E-432.14029345
AAAAAGT2850.030.0155721
AAAAGTA3100.029.0473252
ATGCCGT48050.028.93629644
AAGTACT3150.028.5862584
AGTACTC3250.027.706685
TATGCCG52350.026.55948643
CGTATGC52350.026.55948641
GTATGCC52450.026.50884842
ATCTCGT52200.026.50650637
CTCGTAT52200.026.50650639
TCGTATG52150.026.4887840
AAAGTAC3400.026.4843253
TATCTCG52200.026.46340636
TCTCGTA52300.026.45582438
CCTATCT52200.026.42030534
CTATCTC52200.026.42030535
TCCTATC52500.026.39789433
Produced by FastQC (version 0.11.2)