FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_20.35100000042dc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_20.35100000042dc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120639
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC120539.990964779217334No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT13471.1165543480963869No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG3820.316647187062227No Hit
GAATGTAATGGAGTGTAAAGGAATTGAATAGAATCAATCCGAATGTAATGG1880.1558368355175358No Hit
CTATTCAGTTGATCATAAACAGAGAGTTAGGTACTGCTAATACGGAGAATT1500.12433790067888494No Hit
ATTTTCCTTAGTCAACTGAAGAGGACATAGTTCATTTAACTAACAACGAGC1460.121022223327448No Hit
TCAATGATCAGATAAAAGAACGAATTGATTTCGACAGCAAAAATAACTGCA1390.11521978796243339No Hit
GTACTTCCTTACGTAGACATACCATAGTTAATTTAACCACTCTCCTATGTA1340.11107519127313721No Hit
ATACAAACTTTTCCATTCTTTTAAGGTACTTTGCCCAACATCTAAATTTCC1300.10775951392170027No Hit
CAATAGAAAGATGGATAGATAGTGTCCATGGTTAGATGAATGGATAAAAAA1270.10527275590812259No Hit
AATCAGGATACAGGTGAATTGATTATGGATAGTAACGATTTGGAGAAGGAA1270.10527275590812259No Hit
CATTTCATTTAACAGCTACAGAATACATATTCTTTTCCTCAGGACATGGAT1260.10444383657026335No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.10361491723240412No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCT253.8641003E-545.01161628
AGAGGAC253.8641003E-545.01161620
GCCACGA253.8641003E-545.01161630
AGCACCA206.9992564E-445.01161220
TGAACGA206.9992564E-445.01161218
CGTCTAT206.9992564E-445.01161223
GAGACAA206.9992564E-445.01161231
TACGCAG206.9992564E-445.01161217
AGTTACC206.9992564E-445.01161224
GGCTATG206.9992564E-445.01161227
AGGCTAT206.9992564E-445.01161226
CACCACG206.9992564E-445.01161227
TCGGACT302.151639E-644.99295441
CGGACTT302.151639E-644.99295442
GTCATGC207.013581E-444.992953
GGAGCCT207.013581E-444.992956
GAGCTGA207.013581E-444.9929544
CCCTATA207.013581E-444.9929543
CCTAGAC207.013581E-444.992959
CATGAGC207.013581E-444.9929539
Produced by FastQC (version 0.11.2)