FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_19.35100000042da9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_19.35100000042da9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282646
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC266539.42981680264359No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT52141.844710344388387No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG12530.44331071375501513No Hit
CTATACACATCTTTGAGTAAATGATGGACAATACATGAAGTACTACTAGAC3140.11109302802799262No Hit
TATTAGCTCTAGAATTACTACGGTTATCCGAGTAGTAGTTACCATCAAACA3140.11109302802799262No Hit
CTTTTAATCTCTCATGTACCATTATTCCATGTACCTTCTGTCTCTTATACA2880.10189424226771297No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC207.0137734E-445.013636
CGATCAA207.0137734E-445.013632
TCGCAGA207.026016E-444.99770435
ACACGCA207.026016E-444.99770418
TGCTTCG207.026016E-444.99770425
ACGTTCA207.026016E-444.99770435
CGACATG207.026016E-444.99770429
TACGGAT207.026016E-444.99770430
CCGTAAA207.026016E-444.99770422
GCTTCGA207.026016E-444.99770426
AGTTGCG207.026016E-444.99770410
TACGACC207.026016E-444.9977047
GTTCAGC207.026016E-444.99770428
CGACTTA207.026016E-444.99770440
GTGACTC207.026016E-444.99770415
CCTGAAT207.026016E-444.99770411
GCCGTCT207.026016E-444.99770445
ATCGCAG207.026016E-444.99770434
ACGACCC207.026016E-444.9977048
TACCGTG207.026016E-444.99770420