FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_18.35100000042d8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_18.35100000042d8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252674
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC158396.268551572381805No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT37551.4861046249317302No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG10420.41238908633258664No Hit
AGTGTATACTGTTCATTTTCTTCATACTTAGACCACAACACATGTACTTCT4780.18917656743471825No Hit
CTAAAATACTCCTTAAGTCTTGAACATTACAACATACCATAGGTTGTATGT4760.18838503367976125No Hit
AGTTTTATTGTTGTTTGTTTGTTTTGAAACTACTTGCGATTTATCATTCCT3840.15197448095174018No Hit
ATCAATTATCTGTCTACCTAACATCTATTCCTGTATCTCTGTCTCTTATAC3750.14841257905443378No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT3590.14208030901477794No Hit
ATTACAGTTCAAGTTGATTTTATGAACACTAGATCCTTTGTATTGTCACCT3560.14089300838234245No Hit
TCCTAATGGGAAAGACTACATAAATCCTACGTTTGATCCATCTACTAAAGG3480.13772687336251455No Hit
GAATGGAATGGAATGGAATGGTATGGAATGAAATCAACACGAGTTGAATGG3460.13693533960755755No Hit
AATTAACAATGAAATGCGTAAGTTTAGACATGATTACGTGAACATCTGTCT3460.13693533960755755No Hit
AAACATTATTTTTCCTTCTCAACCTTGCATATGTTGGACGAATGAAGATTC3440.13614380585260058No Hit
AATGACAAGTGACTTAACATTACGTTTCCAGATACCAAAATACCTAATCAC3430.1357480389751221No Hit
CATTATAAACATTGGTGTTGATGATACTTTGATAGGTAATCATCTGTCTCT3370.13337343771025115No Hit
CTTAAAAAGTGTTAGTGTTAGAGTACAAGGTAATAATGCATTTGGCTTGTT3340.13218613707781568No Hit
GAGCATTTCAAGAGATAGAACATGTACGGATTTTGTATTACGATAGACACA3270.12941576893546625No Hit
ACACTAGAATATGATGTAGTTTGTAAATCCACCATACTAAACTGTCTCTTA3230.12783270142555228No Hit
ATGTTTTCTTTACTTGGTTTGTCGATAGTATTCTGTAATACTTCACGCAAC3200.12664540079311684No Hit
GATATCTTGATTCCAATTCTTTTCCTCATTTTTCATTCTCCAAAATGTGGT3160.12506233328320288No Hit
GTTAATCAACAAGGTTACAAATCAAATACTGCAGAATTGGTTATGATGAAA3160.12506233328320288No Hit
CATAGATACACATGCATACACATATCGTCATAGATACATACATAGATCCTG3110.12308349889581041No Hit
TTCATAAACTTTGTTATTTGCTTCTACTGTTTCTTTTCTGTCTCTTATACA3100.12268773201833193No Hit
ATTTACTTTAGACCTTGGTACCTGAACTTTAATCATTCGTAGAACTACTCT2980.11793852948859004No Hit
GATTTATTCAAACAAAACAATGGTTCAACTGAAGAAAAAACTGTCTCTTAT2910.11516816134624062No Hit
GTACTTGATCAAGTGATGAAGTTAAAAAGGCATTTAGATGTGTTGGACAAA2880.11398086071380514No Hit
TTATAAGCCTTATCTTAGAGATTACAAAGGACCTGTTTGTTACTGTCTCTT2860.11318932695884816No Hit
CATTAGTTAAAAGTAGGAATATGGAGTACCATAGCAGGTGTATCTAAACTA2860.11318932695884816No Hit
TCTCAGATGAATTAACTACCTAATCCTGAAGATATACAACTGTCTCTTATA2840.11239779320389118No Hit
TGAAAGGTCAAAAAAGAAGAAGAAGAAGAAGAAAACTACGAATCTCTATAG2840.11239779320389118No Hit
CTAGAAATACCTTATACAGACAAACTCAGATGCAGAATAACTCAAATCCTT2830.11200202632641268No Hit
AAATTGTACAATGCTTAATTCTCAATGCTCTGTTTATACCTGTCTCTTATA2800.11081472569397723No Hit
ATATAATCACCTTTGTCAATGAAAACAGATCATATAGCCTGTCTCTTATAC2790.11041895881649871No Hit
AAATAACATCTTCTAAGTACACAAATTTCCACCCAGTGAGTAAAGAACGAT2760.10923165818406326No Hit
GGATATTTTGATTGCAGATACTTTTGTGGCTTCATTTCTCATTCCATTTGA2720.1076485906741493No Hit
ACATAATTACTCACTGAGCACTTATTAGGTAATATGCTGAGAGCTTTACAA2700.10685705691919233No Hit
ACCTTCATTATGTAGTCCTACACTCCTACTGTGTCTAGTATATGTCACAAT2680.10606552316423534No Hit
CGATTGAATATGATGGAGAATATGGTAAATACGTAATTACACGTATCGATA2660.10527398940927836No Hit
GGAATATTGACATTAAATGCTAAGTGATGTCACAAGGATTTAACCTTTTTG2650.10487822253179988No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT2610.10329515502188591No Hit
TTAAGAAGTAGCTTTCCTCTTATGTGTTCATTCAAAGGTTCAATAGACGAT2610.10329515502188591No Hit
GATGAGTTAAGGGATAAGATACTAAGACTAGCTGAGATTAATAAACCTGTC2610.10329515502188591No Hit
GATCATGTGATTTGATACAATTTAGTTCTTGCAGTGTATAAGCTCAATAAC2600.10289938814440741No Hit
TAGATAAATACCTAGGAAAGGAATTATTGAATTGTGCAGTAACTGTCTCTT2600.10289938814440741No Hit
ATTAACACGTAGTAATGGAGTATTAGAAAATACATCCTGTCTCTTATACAC2530.10012902000205799No Hit
GATATATGGTCATTTGAGCATAATGTGCTTTTTAATACTCTCTTCAGACTG2530.10012902000205799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA302.1451906E-645.0412031
GCACTAC206.9912244E-445.0412031
GGAGCTC351.2057353E-745.00554332
GCAGTCT253.8793158E-545.00554328
TCCCTAA253.8793158E-545.00554327
AGGACCT351.2057353E-745.00554328
CTACGTT351.2057353E-745.00554327
GGAACGT351.2057353E-745.00554330
TTGCGAA351.2057353E-745.00554330
CAGTAGG253.8793158E-545.0055434
GATGTCC302.1569267E-645.005543
CAGCCTA207.0185674E-445.0055432
TAACGTA302.1569267E-645.005545
TGTTGCG406.7720975E-945.0055428
AGCGAGG207.0185674E-445.0055428
TGGGGAG207.0185674E-445.0055430
ATAACGT207.0185674E-445.005542
ACGTACG207.0185674E-445.0055433
TACGTTA207.0185674E-445.0055427
GTTGCGA406.7720975E-945.0055429
Produced by FastQC (version 0.11.2)