FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_17.35100000042d72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_17.35100000042d72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241213
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC180877.498352078868055No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT42261.7519785417867195No Hit
GTATTATTGCACATAGTTGTTACAAGACTTCCGTTGATAAATCAAAAGAGA14630.6065178908267796No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG10820.4485662049723688No Hit
GTGTAAGTTACATTCTTTACCTCAAGTAGTAGAGAAGATGTAGAGGAGAAT10390.4307396367525797No Hit
AGATAAAAGACATCAAACATAACCAACATGAAATCAACTAGAACTCAAAAC8720.361506220643166No Hit
TGTTAAGAGTGCTTAGATTGGTAAGAAAACACAAGATAGTGATACTGTTGT7850.32543851284963915No Hit
GTACACTTATTTCTGTTTGTGGCTTTTTTCTTTTCTCTAGAGTTGTATCAA7380.3059536592140556No Hit
TACTGTAACATCATTTTTCTTCTGATCTTAATACCACAATGATCACTGTCT7150.29641851807323816No Hit
ATTCTATTCTTTTCTATTCCATTCGAATCGATTCGTTTTGATTGAATTCGA7140.2960039467192896No Hit
ACTCATATTCTGTTGATTTCAGTGGCAACAAATATTTCCTTGTAGTAGTAT6350.2632528097573514No Hit
GAATGGAATCATCATCAAATGGATTCAAATGGAATCATTTTCGAATAGAAT6060.2512302404928424No Hit
ATATACACTAAACCTGTAACTGTAAATGAGTGAATTCTGAGCAATGTCTTT5990.24832824101520234No Hit
TTGATAGAGTTTAGGAAGTAGATAAAATGTAGAACCTGTCTCTTATACACA5700.23630567175069336No Hit
ATACATTACTACTAAATCGATACAAGAGCTCTATGATTTCTGTCTCTTATA5540.229672530087516No Hit
GTGTGTATTCATCTTACAGAGTTAAAACTTTCTTTGGATTCAGCAGTTTGG5480.22718510196382452No Hit
ATTCGATTCTTTTCAATTCGTTTCAATTCGATTCTTTTCGATTTGATTCGA5380.22303938842433865No Hit
GGATATTTTGATTGCAGATACTTTTGTGGCTTCATTTCTCATTCCATTTGA5290.2193082462388014No Hit
ACACTAAATTGTGCATGTATAACCTAAAACTCCACAAGGCTAAGCAAAGAT5200.21557710405326413No Hit
TATTAGTATTGGTTGGTATTGAAACAACAGATTCTGAAGAGGATATACTGC5170.21433338999141838No Hit
GTGTTAGTACCATCAATAACATTAGGATTAGCAGTCTCCATTACAAGCATA5070.21018767645193254No Hit
ATATCTTGTGCAATGTTATGTATTGTTCTGTGCAATATCTGTCTCTTATAC4970.2060419629124467No Hit
GATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATGGATATTAAGCTC4970.2060419629124467No Hit
AGACTACGTTGTATATGAATCTACTGTTTATCCAGGTTGTACAGAAGAAGA4950.2052128202045495No Hit
AGACAAGGTAACATTAATAAGAACAAGCAGAAACAACCCAATATAGAAACT4800.19899424989532075No Hit
GTATTAGGTATAGTAGAAAGATCAACGGGGAATGTAAACGTTAAAGCATTT4730.19609225041768066No Hit
GCTATAAACATGTGTGTGCAAGTATCTTTTTTGTATAAGGCTTCTTTTCTT4640.19236110823214336No Hit
TCACTAATCTATCGTTTCATCAGAAGTTCGGTTTTGAATAACATAGTGATG4590.19028825146240044No Hit
TATCTGCATTTGATCAGCATCTTACAGTGTCTTCGTTTTTTCTCTTTTGAT4570.18945910875450328No Hit
CATTTCATTTCACCATTTCATTTCATCATTTCATTTCATCATTCCATTTCC4570.18945910875450328No Hit
AATATGGGGATTTCCTACAGCATGAAAATTGTGTAATTGACTAAACAAATT4540.18821539469265752No Hit
TAGAGGTAGTATCCGAAATTGAATTTGCATACCGACATATATCAGGTTCTA4460.18489882386106885No Hit
GTAGCTAAGAATGTGTGTTTTGTTTGAAAATTTTATGTTGACACAGAGAAA4450.18448425250712025No Hit
TAGTATGTTTGTCATTGATAACAAAACCTTTCCGTATATGAAACTGTCTCT4430.1836551097992231No Hit
CTTAAATCGAGTATCTGATGAAAGTGATGTTAAAAATGCACCTGGTATTAA4280.17743653948999433No Hit
GTAAGGTATTTGAAAAAAATCCGAATGGATGGAATGATAGTTTACGTAATA4260.17660739678209716No Hit
GAGTACGGGATTCTATTTGAATAAGACTTTTGAGTCACATATAACACTTTA4240.17577825407419997No Hit
ATTATATTGCCATGTTATCTTAATGCACCTATATACCTGTCTCTTATACAC4240.17577825407419997No Hit
GTTTTAGTCTTACTAAAGACTGATTTTATGCGAATTACAGAAACAAATACG4210.17453454001235422No Hit
GACTTACTAATGCCATCTTCTTGTCTTCTTACTGTTTTGTACTTCTACTAT4200.17411996865840565No Hit
GATAGGAAATATCCAATGAATGCAGAAAATCAAACGTTTCTTTAAAATGTA4200.17411996865840565No Hit
ACCTTAGCTAACTATCTTAATTTCCAGTATTTACAACTGGATGAACTGTAT4180.1732908259505085No Hit
GAATCAAATTGAAAAGAATCGAATCGAATTGAAAAGAATCGAATCGAAAAA4100.1699742551189198No Hit
TACATATACAGGGTACATGAGATGTTTTGATACAGGCATGCAATGTGGAAT4090.16955968376497121No Hit
TGTTTGTGTATCTTCTATTGAGAATGTTCTATTCACATCTTTTGCCCAATT4070.16873054105707405No Hit
GTTTGTTTGTTTGTTTCATTTCTTAGCATTTATCTTACATCAGATTTACCT4070.16873054105707405No Hit
GAATTAATCGAGGCTTTAAAAATAGGTGAAGAGAATAAACAAAAAGCTATT4060.16831596970312546No Hit
AGGTAGAAGAAAACAGGAACCATTGAAAATACAACATGAACATAACTGTCT4050.16790139834917686No Hit
ATTCCATTAGTCTCCATTCAATTCCATTAATCTCCATTCCATTCCATTCTA4020.16665768428733113No Hit
GTATTGCCTAGGTTTTCTTCTAGTGTTTTTATGGTTTTAGGTCTTACATTT4010.16624311293338254No Hit
ATTATATTGTCTGTTTTGTCAACACGAAAAATACCAAACTTAGAGGCTGCA3970.16458482751758818No Hit
ATCCACATGGTATCATCAACTCTTTCTCTTCGATCATTGAAAGTAAGATAG3960.16417025616363962No Hit
ATTCTATGGACATGTTAACAGAAGATTATAATTCCTGTCTCTTATACACAT3880.16085368533205094No Hit
GCTATTTTTCATTCCTTGTTTCTTGCTCATTATTTTTTGTACTGAGCTGAA3870.16043911397810234No Hit
ATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATGGATATTAAGCTCT3850.15960997127020518No Hit
GAATGGCATGGAATAGAAAGGAATTGAAGGGAGTGGAATAGAATGGAATGG3830.15878082856230802No Hit
CTTTATAAAGTAATCGCTTTCTTTTGTCTGTTTTCTTGCGATTTTTGTGAT3810.15795168585441083No Hit
TTCTTGGAGCATGTAAATCTGTTACAATGAACATGTCATTTTTATAGTCCT3710.153805972314925No Hit
GCTTTCATGATCTTTATTGTTAACAACAGAGTACCTGTAAGTACAAACGAT3700.1533914009609764No Hit
GATATTACTGTGACGAATTCCATTTGCTTTTAAGAGAAGAACAAACAGCCT3670.15214768689913064No Hit
TATGTTGGGTGATAAAGAAACAGTATACGATCAGAAGCATACCGTTGTAAA3660.15173311554518204No Hit
CTTAATACTCTGGTTTGCTTTTTTGTTCACGTTTAGTTTGCTTGTTTTAAT3650.15131854419123347No Hit
TATTAGTGTTACATCATTGTCTTCTTGTTTTACTTCAGCTTTACCACTGTC3640.15090397283728488No Hit
ATCTTATGTTAATTCACCAAGATGATTGGTTACATGATCTGTCTCTTATAC3630.15048940148333628No Hit
TGATAAGTCTGGTAATCTACCATTAATCCATGTGCAATCAATTCATACCAA3610.14966025877543912No Hit
CTCTTGAAAAGAAAATTAACCAAGTAAGTATCTCCTCTAGTAAGTCTGTCT3580.14841654471359336No Hit
TATTTATCTTGATCAGCTAAAACTTTTTTCAAAGTAACACCTGTCTCTTAT3560.1475874020056962No Hit
TTGAATGAGGTATTTCAGGGACTTTTTCAGCTATTCAAGTGATCACAAAGA3520.14592911658990187No Hit
TATTTATCCTTCACAATGCAGACGAAGACGTTCTAGAACTAATAAAAGAAC3490.14468540252805612No Hit
CTAATAATCAAATGGAATGAGAAATGAAGCCACAAAAGTATCTGCAATCAA3480.14427083117410752No Hit
AAAATTCACTTGGAATTCATGTTTTGTGATACGTTAGATTGGAATCCATTT3470.14385625982015893No Hit
CATTTAAAGAACCAACCAAATGATGTTTCTTTCTCTCACTCTCTGTCTTTC3460.14344168846621036No Hit
CTAGTGATTTGAGCATCTTTTATGTATTTGTTGACCATTTGGATGTCTTCT3450.14302711711226176No Hit
AATCATTACAGTAAAAACATTTCTGTGTCACTCTGTCCTTGAAATAGCACT3420.141783403050416No Hit
GTTCTATTGGAAAACCTTTTAAGCTACCTTAACGAGAAGAAGAGGTCATTT3420.141783403050416No Hit
ATATAGAAGTGTTCTAGTCTATGATGCATTTAGATTTGGTACCTGTCTCTT3390.14053968898857025No Hit
TCTTACAACAACACACAAAGCAAAAGATAAAGAAGCATTGTAAAGGTAGAT3370.1397105462806731No Hit
ATGTCCTCATATGATCCAATACACTATTCACGTCATTCATAACATTTTTGC3370.1397105462806731No Hit
ATTCTATTGTGACCATTATCAGAGATATTCCGGATGCAAAGGAAAGTGGAC3360.13929597492672452No Hit
CTGCATTACCTTGAAAGTTTTGAAGTTCAAGCATGGTGTTTTCAAAGTTCA3350.13888140357277592No Hit
GATTTAAGATGGACAACTTCATCAGAAAACGGCATTAAAAATTATGTCGTT3310.13722311815698157No Hit
ATATTTACGTTTCTTTGGAATTGGAGAATCTAGATTAGTAACGGATATAGC3300.136808546803033No Hit
TTTCTATTCTATCTTTGTGTTCACAATCTCCACCTGTAATTCTTAGTAAAG3280.13597940409513584No Hit
GTGCAATTTTTCCATTGCTTTTTTACAACTTCAATTGCAACATTTGATTTA3280.13597940409513584No Hit
TTTTGATGCTATTGTAAATGCAATTGTTTTCTTAGTTTCAATTTTGGTTTG3270.13556483274118725No Hit
GTTATTTTCAATAACATGCACCCATACCTAATAAGGTTTATCACTGTCTCT3260.13515026138723865No Hit
CTTATGGCTTTATATCTGCCTATACCTTTTGTTTCTGTCATAAATACATCT3260.13515026138723865No Hit
GTCCACACTGATAGATAAAGACTTACACCAAATGTTTTGCATTCAAATATG3250.13473569003329008No Hit
TACCAATGTTGGTCGTCATCATAAAATAAAAGCCGGAGATACCATTGTAAC3240.1343211186793415No Hit
ACTTACAACCAGACACACTAATATTCCTACAGTGGAACATAAGAACAATCA3200.13266283326354716No Hit
GAGTAATTCATTCTCTAACCAAACTTTGGCTCAGATTGAATTGTGGACTAA3190.13224826190959857No Hit
ATCTTGATACACTTGCTTATATTCACCTTATGCTTCAGTCATGTCGTTTAA3140.13017540513985565No Hit
GTATCAAAGTAAGTAAGATCTTATGCGATTAAGATCCTATGTATCTGTCTC3120.1293462624319585No Hit
ATATAATCCTGAGGTTACATGTTTTTAGAGCAGTTTCTCTGTGATTCTTCT3110.1289316910780099No Hit
ATGGAGACCATAATACAATGAAGCATAAATAACATGACTGAAATCATGTCT3110.1289316910780099No Hit
GTCTTCGTTTTTTTCTCTTTTGATTTATCAACGGAAGTCTTGTAACAACTA3080.12768797701616413No Hit
TGCTAAATCATTTCATGTATTGAAAGAATTGCTATTTCCAACAATCCAATG3010.12478597753852404No Hit
AAATAGGGGAGAGAATAAGGGTTAGAGAGAAATAGATGAAATGGAATTTCC2990.12395683483062687No Hit
ATACATGTTTTTCCTGTACACATACCCATGATAAGTTTTGATAAACTAGGC2990.12395683483062687No Hit
TAATACAGCTGTGCCTATTATTTTGGTAATCTGAAGCATGGAAAATCTTCC2990.12395683483062687No Hit
GTTAAATGTAATTGTTCATTGTAGTTTCTTGTGATCCTTTTTATTTCTGTG2980.1235422634766783No Hit
GATTACATTCATAAAATCACAACCGATATGGTTAAAAGTTATGACGCTGTC2970.1231276921227297No Hit
GAAGATAATGGAGACATTTTCGTTTTTCAAAAAATAACCAAACATAGACTC2970.1231276921227297No Hit
GTATGGTTTTTGTTTTTGTAGTGTGGTATGTTTGTAGGGTTAAGTTTTTTT2960.12271312076878112No Hit
CAATAGAACAAATAGACACAAAATCAGTAAGGATACAGAAGACTTGAACAA2960.12271312076878112No Hit
TTCTTAAAGAGTTCCTTTGATGGAAACAAAGTAAGCATTTTAGATCAGAAG2950.12229854941483254No Hit
ATTCTAACTCATCTTCTTCAATATGTCTAAGGTTTCAGTAATTCTGAACTA2940.12188397806088395No Hit
TTGCAGGATATTGTGGAAACATTGATTATTGTAATCACTGTCTCTTATACA2940.12188397806088395No Hit
AATCTAAAATGAACATGTAAATGAAAGGATACAACTCCTGTCTCTTATACA2930.12146940670693536No Hit
CTATTGTTTTGTGTAGCATACCATTCTTTCTAGTGTTTATTCCTGTCTCTT2930.12146940670693536No Hit
AAGTTGTACTAATTGCATTATCCAACTGTTGAAAGAACTGTTAAGTCTGTC2920.12105483535298678No Hit
CTTAGCTACTTAGTAACATTCATAGAGCTTAATATCTGTCTCTTATACACA2920.12105483535298678No Hit
AAACTGTATTCTTCTCCAATTTCGAATTCATCTTCCTCATTGATTTCTGAC2910.1206402639990382No Hit
AATCTAGAGAAACAGAGGTATTTTATGATCATGAACACGAAAAAAATCTTG2900.12022569264508962No Hit
GTTAATTTCCTGAAGATCTACTTAATCTGTGAAATACAAATTGTATCTGCT2890.11981112129114102No Hit
TTTTATGGTAAGTCTGTTCATTCCAAATTTCTAATTGATTTGATTCTGTCT2890.11981112129114102No Hit
CTATACATCAGTATTCTCTTTGTGGAAACTGCTATTCCTCTAATTACTCTA2880.11939654993719244No Hit
GATTGTTGCATCTCATTTTGTTAAGAAAGGCTATTAACCTTTTCTTTTTCT2860.11856740722929528No Hit
GAGATACTAACGATTCACGATTCTATCAGTGTGCCATATCTGTATGTTGAA2820.11690912181350094No Hit
TAACATTACCCGATGTTAAGTTAGGGAACATAAGTACATTAACATCTTCAT2820.11690912181350094No Hit
AAGTTATGCACCATAAGTGTGGTGTAGTATTCTTCATAATCTGTCTCTTAT2810.11649455045955234No Hit
GCTTAGTGTATTTGAAATAGCTATTTTCTCACTGTGAAAAAATAAGCTGTC2800.11607997910560376No Hit
AGTTTCTTGGCAATAACTTGCTCTAATTGTTCATTACTTTGGTTATTAAGT2800.11607997910560376No Hit
ACAGAAATTTTTCAGAAAGAAGGAAAAGAATATGTCAGAAACTCAGATCTA2790.11566540775165518No Hit
GTGTCAACATAAAATTTTCAAACAAAACACACATTCTTAGCTACTTAGTAA2790.11566540775165518No Hit
CTACTACAGAAAAAAGATATGATCAATAGAAAGCGCACCATAATTAGATGC2770.114836265043758No Hit
ATGCAAAACAGGAAAAAAAATGAAAGAAAAAGACTTCCAAAAACAAGAACA2750.11400712233586084No Hit
GATTATTGAAGTTACTGGAAGACTTCTATAAGAAGAGATATCCACATTCAT2750.11400712233586084No Hit
AGAGTCTTTTTTGGTCAGAACAAACTGAGAGTTATGTTTTTGTTGTAAGAA2750.11400712233586084No Hit
GTAAGATACATTAGAAGTGAAACATAATCCAAAACACGTCTCAAACTCAAG2740.11359255098191226No Hit
GACTTGTGGATTTGGTATTATGCATATTTGAAGATGTTTCTTCTTCCTTAA2710.1123488369200665No Hit
ACTGAGTACTTCTAACATTTGATCATCTTTCAAATCGGAATAGTCGATTCT2700.11193426556611792No Hit
GAGATGTATGGTGCAATAGATACAAAAAATTCATCGTCTGGCAAAGTATAG2700.11193426556611792No Hit
CTATTAGCTTAAGTTTAGAGTTCTAATTCTTTTTACTGCTCAATCTCTGTC2680.11110512285822075No Hit
AAATAAGGCATGGAGAAAAGGAATGTAGTACACTAATAATCAAATGGAATG2680.11110512285822075No Hit
GAATTACTCAGTGAATGATACATAGAATTACAGGATACCATACCTGTCTCT2680.11110512285822075No Hit
CAGATTATCTATCTGTGATTATCGATCACAGATTATTAACCTGCTTACCTG2670.11069055150427216No Hit
ATCCATTGCTTTATCTTTTTTGCTAAAGGAGAAGAAATGTTTTGCTAATGT2640.1094468374424264No Hit
GCTCTATGTCATCTTATTTGTTTCACTTTTCATCTTGATACAAAGTCTTTC2630.10903226608847782No Hit
CTCTAGTATCCTCTGTTCTACTTTTTATTTCCATGAGAACAACTTTTTAAG2630.10903226608847782No Hit
AATCATTATTGATTGGAATTAAGCGTGAGCCTCTGTACTTGTGTTTTGTTG2620.10861769473452924No Hit
ATTTAAAACATTACAAGAAACATTACCTCAAGATCTGTCTCTTATACACAT2620.10861769473452924No Hit
AACTAAAACAGTTGACAATTTTATTTTCACATTTCACACTGTCTCTTATAC2570.10654483796478631No Hit
GTTTGGAATAGGTATCAATTAACGAATCACAAAACAAAGATAGAAATGTCT2560.10613026661083773No Hit
TTATCATAAGCATAAAACTGCTGCATATTCCCAAATTATTTCTACTTTGCC2560.10613026661083773No Hit
ATCCTGTTCAATACCCCAAGCAATAAGATTTGGGTTCTTAATATGAATCAG2560.10613026661083773No Hit
ATATAATACATCGCTGCCTGAAAATAAGGATGATTGTCTTTGTTCATTACA2550.10571569525688915No Hit
ACATTAATCACTGCGTATTACATTGTAACCAACCATGTAACTGTCTCTTAT2550.10571569525688915No Hit
TTTTATTGACGAATAAAGACATTAAGCTAAACTTTAAATGACTAGCTTCAA2520.10447198119504339No Hit
CTCTAATACTTTTTGCCCTTTGTATTTAGTTGCTATGTTATTTGGTACATA2510.10405740984109481No Hit
AATTAGCTTCATTTTATGGATAAACTGAACTGAGGTATATCTGTCTCTTAT2500.10364283848714623No Hit
GATAAAATGTTTTGGAGTAGGGATAAGCACTGTAGATGCTAAGACATATAT2500.10364283848714623No Hit
GTGTAATATAGGCATCATTAGTTTCTACCAATCGGCTAAGTTCACCGAATG2490.10322826713319763No Hit
CTAAAAAGTAGTTTTGTTCAAATTTTCTTACAATGCATTACTCGCTCACAA2490.10322826713319763No Hit
ACTTGTGTAAGGAAACATAGGTTAATTTACTTTTACACCTGTCTCTTATAC2470.10239912442530047No Hit
GATTAATACTATCCATACTTGCGGTATCTTCAATATCTGTCTCTTATACAC2470.10239912442530047No Hit
AAAACAAACAAACAAACCTAATTTGTTGATACGTCTATGTACCTCATAAAA2460.10198455307135189No Hit
AAACTGGTATATAGAAAATGGTATCCCAACTATCAATCTGTCTCTTATACA2440.10115541036345471No Hit
AGTAAACTCTTTTTACCCATTAGTAATTGACTTTGAAACAGGATATGCCTT2440.10115541036345471No Hit
TAACACAATGGTGTTTGTGTATCTAAACCCTTATACATACAAAAGGTACAG2430.10074083900950613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGA302.1493197E-645.027396
ATTAGGG302.1493197E-645.027392
GGGTCCC302.1493197E-645.027396
TCTTACG302.1493197E-645.027392
AGGGTCC302.1493197E-645.027395
TCAGCTC302.1493197E-645.027393
TAGGGGA302.1493197E-645.027394
CTATTAG302.1493197E-645.027391
ATGGGTT207.001169E-445.0273865
GTGCACA207.001169E-445.0273861
CTGTAGG207.001169E-445.0273863
CTGAGTA406.7393557E-945.0273862
ATCAGAC253.8676837E-545.0273862
ATCGTAG253.8676837E-545.0273863
CTAACTC406.7393557E-945.0273864
CCGTAGT253.8676837E-545.0273862
GAGCGTT207.001169E-445.0273865
TGCCTAG406.7393557E-945.0273865
GCCTAGG406.7393557E-945.0273866
TGGATCG207.001169E-445.0273865
Produced by FastQC (version 0.11.2)