H23LNBCXX_l01n01_bir_1228_pcontrolb_16.35100000042d59.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_16.35100000042d59.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18210
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	892	4.898407468423943	TruSeq Adapter, Index 12 (95% over 21bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108	0.5930807248764415	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.5820977484898407	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	104	0.57111477210324	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.5656232839099395	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101	0.5546403075233388	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	98	0.5381658429434377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.47226798462383307	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	81	0.44481054365733114	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	79	0.4338275672707304	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75	0.41186161449752884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.3734211971444261	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	60	0.3294892915980231	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	60	0.3294892915980231	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.29654036243822074	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.29654036243822074	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.2910488742449204	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.2800658978583196	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.2471169686985173	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	41	0.22515101592531575	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	39	0.214168039538715	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.214168039538715	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	38	0.2086765513454146	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	38	0.2086765513454146	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	36	0.19769357495881384	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.18671059857221306	No Hit
GTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.1757276221856123	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAA	29	0.15925315760571115	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	28	0.15376166941241076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	26	0.14277869302581	No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	26	0.14277869302581	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	26	0.14277869302581	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	25	0.1372872048325096	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	24	0.13179571663920922	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA	24	0.13179571663920922	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	23	0.12630422844590886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT	19	0.1043382756727073	No Hit
GTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAA	19	0.1043382756727073	No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	19	0.1043382756727073	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	60	0.0	44.999996	45
GTATGCC	85	5.456968E-12	31.764706	42
TTGCAAT	85	5.456968E-12	31.764706	32
ACTTGCA	85	5.456968E-12	31.764706	30
CCACGAG	85	5.456968E-12	31.764706	20
TATGCCG	85	5.456968E-12	31.764706	43
CACGAGA	85	5.456968E-12	31.764706	21
CCCACGA	85	5.456968E-12	31.764706	19
ACGAGAC	85	5.456968E-12	31.764706	22
CGTATGC	85	5.456968E-12	31.764706	41
CTTGCAA	85	5.456968E-12	31.764706	31
CGAGACT	85	5.456968E-12	31.764706	23
TACTTGC	85	5.456968E-12	31.764706	29
CTACTTG	85	5.456968E-12	31.764706	28
GAGACTA	85	5.456968E-12	31.764706	24
ATCTCGT	80	8.54925E-11	30.9375	37
TCTCGTA	80	8.54925E-11	30.9375	38
TCGTATG	80	8.54925E-11	30.9375	40
CTCGTAT	80	8.54925E-11	30.9375	39
GCCCACG	90	9.094947E-12	30.0	18

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content