FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_13.35100000042cfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_13.35100000042cfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49460
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC871417.618277395875456TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT637512.88920339668419No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG7501.51637687019814No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2780.5620703598867771No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2660.537808329963607No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA890.17994338859684594No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA740.14961585119288315No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA650.13141932875050547No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT570.11524464213505863TruSeq Adapter, Index 12 (95% over 22bp)
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA570.11524464213505863No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG550.11120097048119691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10100.044.10296245
TATTTTA300.00510368129.9959567
ATGCCGT17500.025.45371244
TATGCCG18450.024.14308743
GTATGCC18500.024.07783342
CGTATGC18500.024.07783341
ATCTCGT18150.024.04634337
TCTCGTA18150.024.04634338
TCGTATG18250.024.03785540
AAAAGTA1500.024.0210532
CTCGTAT18200.023.98028439
CTTGCAA18700.023.94062231
AATCTCG18150.023.92239436
AGACTAC18650.023.88417825
ACTACTT18650.023.88417827
ACTTGCA18750.023.87678130
GCAATCT18300.023.84924334
CGAGACT18500.023.83462323
CACGAGA18600.023.82743321
ACGAGAC18600.023.82743322
Produced by FastQC (version 0.11.2)