H23LNBCXX_l01n01_bir_1228_pcontrolb_11.35100000042cb6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_11.35100000042cb6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38579
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	7039	18.245677700303272	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	484	1.2545685476554602	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	211	0.5469296767671531	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198	0.5132325876772337	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148	0.38362839886985145	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	132	0.34215505845148914	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.3395629746753415	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127	0.32919463957075096	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	123	0.31882630446616034	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109	0.2825371316000933	No Hit
TTACTCTATCTAGAGATTCGGGAGAAGATAACAAGATAATTAAGCCTAGAA	105	0.27216879649550274	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	100	0.2592083776147645	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.24884004251017394	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	88	0.22810337230099276	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	81	0.20995878586795927	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.20995878586795927	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78	0.20218253453951632	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.19959045076336865	No Hit
CATATTGATATACTGGCAAAAGGTATAAAGAAAAAACACTTGAAAGATTTG	74	0.19181419943492573	No Hit
GTATTTTGAAAAACGACTAAACCAATGCTTTTTCACTGAAACTGAAATTTA	72	0.18663003188263044	No Hit
ATCTATACGCATCTTAGATCACTGTATATTCAGTTCTGAAAAGCTTCTAAT	71	0.1840379481064828	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.16848544544959693	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	62	0.16070919412115398	No Hit
ATACTGAGTCTTTCAATTCATGAACATGGAATAGCTTTCCAGTTTTTATGT	62	0.16070919412115398	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.1503408590165634	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	56	0.14515669146426813	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	55	0.1425646076881205	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT	52	0.13478835635967754	TruSeq Adapter, Index 12 (95% over 22bp)
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	52	0.13478835635967754	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	52	0.13478835635967754	No Hit
AATATGTAAAGCAGGGAATTCTTTGCATCCATTAAATCGATGTTCATAAAT	51	0.1321962725835299	No Hit
AGAATATACTTCGTCTAGATCCGAAATACGTTTTTATTTCCCCGGTATTCT	51	0.1321962725835299	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	49	0.1270121050312346	No Hit
CATTATTTCCCATATCTAATTTCAAGATTCCCTGACTCTCACTTTTTCTAA	47	0.12182793747893932	No Hit
ATCATAGATACAGTCACATACTGCATAATAATGTTTTGTTCAACAATGGAC	42	0.1088675185982011	No Hit
ATCCAGTAATTATGGACCATTGTATTTTCATTTGTCTCCATGCACTTAATT	42	0.1088675185982011	No Hit
AGCATATATATAACAACGGAACCGAAGAAGTAATACGTAGAGGGAAGAAAA	42	0.1088675185982011	No Hit
TACCTTCTTCATACGTCATCATTTTGATATAGGCAAAAGAGATTCCTTGGT	41	0.10627543482205344	No Hit
AATCATTATACAAAAAGAGACACTTGTACTATGTTTATCACAGCACTATTC	41	0.10627543482205344	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	40	0.10368335104590581	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	40	0.10368335104590581	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCTA	35	1.16122465E-7	45.05065	2
CTATCTA	35	1.16122465E-7	45.05065	6
AGCCTAG	35	1.1731936E-7	44.992214	43
GGAGAAG	35	1.1731936E-7	44.992214	21
TAAGCCT	35	1.1731936E-7	44.992214	41
GCCTAGA	35	1.1731936E-7	44.992214	44
ATCTAGA	35	1.1731936E-7	44.992214	8
ATAATTA	35	1.1731936E-7	44.992214	36
ATTCGGG	35	1.1731936E-7	44.992214	16
TAACAAG	35	1.1731936E-7	44.992214	29
GATTCGG	35	1.1731936E-7	44.992214	15
TAGAGAT	35	1.1731936E-7	44.992214	11
ACAAGAT	35	1.1731936E-7	44.992214	31
CTAGAGA	35	1.1731936E-7	44.992214	10
AAGCCTA	35	1.1731936E-7	44.992214	42
AGATTCG	35	1.1731936E-7	44.992214	14
TTCGGGA	35	1.1731936E-7	44.992214	17
TCGGGAG	35	1.1731936E-7	44.992214	18
TGCCGTC	835	0.0	43.645145	45
TTACTCT	40	3.3142533E-7	39.419315	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content