H23LNBCXX_l01n01_bir_1228_pcontrolb_10.35100000042c80.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_10.35100000042c80.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26091
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	3749	14.368939481047105	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	251	0.9620175539458051	No Hit
TTTTTATACTGATCGTGCTTCTATATCAATTAGAAGCTACATTGAGTAGGC	142	0.5442489747422482	No Hit
AAAGAATACAGTCTATTCTATCAACTCTGAACAAACAACTAAAAGAAATAG	126	0.4829251466022766	No Hit
ACTTTCAACAATAGATAGATCATCCAGACAGAAAATCAATAGAAAACACTT	123	0.47142692882603193	No Hit
AGTTATTATGGTGTAGATATTTCGGAAAACTATATTGAAGAGGCTACGAAA	120	0.4599287110497873	No Hit
TAATAAAATTGCTTGAAAGAACAAGGTTTGGGGATACTGGTCTTTGGAAAT	114	0.4369322754972979	No Hit
ATGTACTTGCATATCTATCACCACTATTGTAATCTGCTCAGTAAATAGCGT	113	0.43309953623854974	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108	0.4139358399448086	No Hit
TATTTAATCAGCATATGTTTCACATTGCTTTAGTGTTTTACTCTTTCTTAC	106	0.4062703614273121	No Hit
GTGTATGAACATATTTCTAAGTCAGTGTATCCTCAGCAAATTTATTTCTAC	94	0.36027749032233336	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.337281054769844	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.32961557625234755	No Hit
TTTTTCTAGTTCTGTGAAGAATGTCATTGATAGTTTGATGGGAATAGCATT	85	0.32578283699359933	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.3142846192173547	No Hit
ATAAATAGTTCATCTAAAGTATGGAACAGGAAAATAGAGATGCTGCTGAGT	82	0.3142846192173547	No Hit
ATATGGAACTAAAATGTCAAATCAAGGAAGCAAAAGAAGATTTACAAAAGA	82	0.3142846192173547	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT	81	0.3104518799586064	TruSeq Adapter, Index 12 (95% over 22bp)
TTATTAATGAGCACTTGTATATTTACAGGAAATGGGATAAGCAACACACAA	80	0.3066191406998582	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	80	0.3066191406998582	No Hit
ACTATTAGGTGCATAGTTTTTCCACTTTTGGTTTGAAATGTTAAAAATTGT	79	0.30278640144110996	No Hit
GTAGTATGACATAAGGTTCTAAATCGTGTCACCATATTGATTTAATTGTAA	79	0.30278640144110996	No Hit
ATGTAAGCTGTTTTTTTAATAACCTTGAGGAATTTATCTTTGTTCCTAGTT	78	0.29895366218236175	No Hit
GGATACTTATGTCAAAAAAAGAAGATGTAATTGTATTAGACGAAGTCATTG	77	0.2951209229236135	No Hit
ATTTAAGATAGTGATTCTGTAGAAAATCAGGTACATTTGATTGACCTTGTC	76	0.29128818366486525	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.2721244873711241	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	68	0.26062626959487945	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67	0.2567935303361312	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.252960791077383	No Hit
ACAGTAAAAGGTATCAACTTAATACCAGATGTTGACTTTACAATCACTGAT	63	0.2414625733011383	No Hit
ATTCAATACTAGCTCATTTGTTTACAGAATGGAGAAAATTAAAAAGAAGCT	61	0.23379709478364186	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.23379709478364186	No Hit
ATATCTCACCACATAGTTGTAGTTTAGGTAATAAGTTAATGTGGTGATATA	60	0.22996435552489364	No Hit
GTTCATGTATTTGATATTCGTTTTAGATATCGCAGATTAATCTTTGTAAGT	60	0.22996435552489364	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	58	0.2222988770073972	No Hit
GTATGTTTATATCCGGAAAAAGTATAGTCTCCTAAACTGTTGGTATGTATG	57	0.21846613774864895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57	0.21846613774864895	No Hit
GACCAAATAGATAACATAGGCCAATAAGTAACCTGATACATCCACTTTAAA	52	0.1993024414549078	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.19546970219615958	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	51	0.19546970219615958	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	49	0.18780422367866315	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49	0.18780422367866315	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC	48	0.1839714844199149	TruSeq Adapter, Index 12 (95% over 24bp)
AAACTAATCTTGCTGGAATTCAACACAATTTCATCATTTCAAATCAGGACA	47	0.18013874516116668	No Hit
GAATCAAATAGAATCATCATTGAACAGAATCAAATTGGATCATCATCGAAT	47	0.18013874516116668	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.18013874516116668	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	47	0.18013874516116668	No Hit
CAATTCAATTAGATGCATTAATCTCTCTAGTGAATGATGGCAAAGCAAAAG	42	0.16097504886742553	No Hit
ATCTTGGAGTTAGAGTTACGATAATAGAAGTTGCAGATGACATTTTATTAA	41	0.15714230960867734	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	40	0.1533095703499291	No Hit
CTCCTAACATTCTATAGAAATAAGCCTGGAGAGGAAAAGAATGTTCCTAGA	40	0.1533095703499291	No Hit
GTAATTAACCATTGTGATGTCACAAGTCAACAATATCATCATAATGCTTAT	39	0.14947683109118087	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.14564409183243263	No Hit
GGATAAATGCTTAACATGATAGAGACTCCATTTATGCTGATGTGATTATTA	38	0.14564409183243263	No Hit
GTACGGGATTCTATTTGAATAAGATCATGAGATTCCACCTATACCTCCACC	38	0.14564409183243263	No Hit
ATAAGATACAATAGGGAAAAGATGGTGTCTTCGATAAGTAGTGGTAAGAAA	37	0.1418113525736844	No Hit
ATGTAAGAACTGACACACAATAGAAGCTCATTTCAGTTTGTTTTAAATCTG	36	0.1379786133149362	No Hit
AAATGTTACAGTTTGAAAATAAGACAGTAATGTTCTGTTACTTCTACTACC	35	0.13414587405618797	No Hit
GTTTAAACTACTACCTTCAAAATCCACATTTCTAAATGCATCATCTCCTAT	35	0.13414587405618797	No Hit
ATTCTTTTCAATTCGATTCTTTTCGATTCGAGTCTTTTTGATTCGATTCAT	35	0.13414587405618797	No Hit
ATATATATGGTAGCCTTTAAAACCTTATTAACCTAAGAACTTAGCTATATC	34	0.13031313479743972	No Hit
CAATTGGTTACAAAAATCTGTATTGAAAAATTTCAACACAATTTAGTATGG	34	0.13031313479743972	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	33	0.1264803955386915	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	32	0.12264765627994327	No Hit
GGATAATATGAATGGGAAAAACTGGAATAAAATTCGTTAATGACATCTATT	32	0.12264765627994327	No Hit
GTCTTATAGTTGTTTTTCTTTTTCTCTCTTGCTGTTTTCCTTTGTGTTTCA	32	0.12264765627994327	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	31	0.11881491702119505	No Hit
GGTTATTACAAATGGACCCAATGGATTTTCTTACAGTTATTTCAATCCTGG	31	0.11881491702119505	No Hit
ACACATGCTAGTAGAATTTGTAATTTCTTCGTTTTTATTTAGTTTTGAGTG	30	0.11498217776244682	No Hit
ATATTAAGCTTGTTCAGGCAGAAAACGCTTTAGCTAATTAATCTATGGAAA	30	0.11498217776244682	No Hit
ATTTTAATGTCTGTAGAATCTGTAGTGATATCATCTCACTCATTACTGATA	29	0.1111494385036986	No Hit
GTACAAATGTAAATGGGGAGAAATAGTGAAATCTTTTCTGTTTGTCATCAT	29	0.1111494385036986	No Hit
ATATTACATTTTCTTTTTTTGCCAAATAGTTTGTCAAATGTGTATAGTCCA	29	0.1111494385036986	No Hit
ATTAATGAGAGTTAGCTTAATATACAGCAAGTTTTACATTCCTGTATATTA	28	0.10731669924495037	No Hit
GACTGACTCAATGAATCCTTAATCTCATCAAAATAAGATAGACGGTCTTCT	28	0.10731669924495037	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10348395998620215	No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10348395998620215	No Hit
CTCTTATCACTATGTGAAATTTCTTGTAAAATTTGGCTGAATGTATGTAGC	27	0.10348395998620215	No Hit
GGATAATTAGCTAGAAATAACAAAAAAAACAGCAAAAAAGAACAAAACAGA	27	0.10348395998620215	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGGT	20	6.906947E-4	45.000004	6
GTAGATA	20	6.906947E-4	45.000004	13
AAACTAT	20	6.906947E-4	45.000004	27
CTATATT	20	6.906947E-4	45.000004	30
TATATTG	20	6.906947E-4	45.000004	31
CGGGGCA	30	2.084662E-6	45.000004	4
GTGTAGA	20	6.906947E-4	45.000004	11
AGAGGCT	20	6.906947E-4	45.000004	39
AAGAGGC	20	6.906947E-4	45.000004	38
TTATTAT	20	6.906947E-4	45.000004	3
GAAAACT	20	6.906947E-4	45.000004	25
GAAGAGG	20	6.906947E-4	45.000004	37
TCGGAAA	20	6.906947E-4	45.000004	22
GCTACGA	20	6.906947E-4	45.000004	43
ATTATGG	20	6.906947E-4	45.000004	5
AACTATA	20	6.906947E-4	45.000004	28
ACGGGGC	30	2.084662E-6	45.000004	3
TACGAAA	20	6.906947E-4	45.000004	45
TGTAGAT	20	6.906947E-4	45.000004	12
AAGAAAT	20	6.906947E-4	45.000004	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content