FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_1.35100000042b74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_1.35100000042b74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83154
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC2815833.86247203982971RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT6860.8249753469466291No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT6260.7528200687880319RNA PCR Primer, Index 38 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG3040.3655867426702263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.35235827500781686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.34394015922264715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.25254347355509055No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1730.20804771869062222No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1650.19842701493614257No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1510.1815907833658032No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC1450.1743752555499435RNA PCR Primer, Index 27 (95% over 24bp)
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1440.17317266758063352No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1360.1635519638261539No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.1527286721023643No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1220.1467157322558145No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.13950020443995478No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.12987950068547516No Hit
TATCAACGCAGAGTACGGGTCGAGGGAGGTCCTAAGAAGAAACGTAAAGTT1070.1286769127161652No Hit
GTATCAACGCAGAGTACGGGTCGAGGGAGGTCCTAAGAAGAAACGTAAAGT1050.12627173677754527No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.12386656083892536No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1010.12146138490030545No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA990.11905620896168555No Hit
GTCCTAAGAAGAAACGTAAAGTTGGTGGCCCAAAAAAAAAAAAAAAAAAAA980.11785362099237559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32000.043.10156245
ATGCCGT33100.041.53323444
CATCTCC33200.041.137059
CCCACGA33500.041.10447719
CTCCGAG33350.041.08695612
ATCTCCG33300.041.0810810
CACGAGA33600.041.04910721
ACGAGAC33550.041.0432222
CCACGAG33550.041.0432220
GTATGCC33500.041.0373142
TCCGAGC33450.041.0313913
ACATCTC33450.041.031398
CTTATAC34150.040.980971
TATGCCG33550.040.97615443
GAGACGA33550.040.97615424
CGAGCCC33550.040.97615415
CCGAGCC33500.040.9701514
CGAGACG33500.040.9701523
TGCTATC33450.040.96412733
TCTCCGA33400.040.9580811
Produced by FastQC (version 0.11.2)