FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_96.35100000042b4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_96.35100000042b4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5008
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC78315.634984025559106No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT751.4976038338658146No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG240.4792332268370607No Hit
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC120.23961661341853036TruSeq Adapter, Index 4 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC750.036.045
CATCTCC850.034.4117629
TATACAC950.033.1578943
TTATACA950.033.1578942
ATCTCCG900.032.510
ACATCTC900.032.58
ACACATC900.032.56
ATACACA900.032.54
ATGCCGT853.6379788E-1231.76470644
CTTATAC1000.031.51
AGCCCAC950.030.78947317
GTATGCC950.030.78947342
CACATCT950.030.7894737
CAGCAGC950.030.78947329
CAGCAAT950.030.78947332
CCAGCAG950.030.78947328
CCACGAG950.030.78947320
TATGCCG950.030.78947343
GAGCCCA950.030.78947316
CACGAGA950.030.78947321
Produced by FastQC (version 0.11.2)