Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pb_95.35100000042b31.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8286 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC | 2009 | 24.24571566497707 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT | 188 | 2.2688872797489745 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG | 57 | 0.6879073135409124 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC | 27 | 0.3258508327299059 | TruSeq Adapter, Index 4 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT | 13 | 0.1568911416847695 | RNA PCR Primer, Index 18 (95% over 21bp) |
TATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTT | 9 | 0.10861694424330195 | RNA PCR Primer, Index 4 (95% over 21bp) |
CTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT | 9 | 0.10861694424330195 | RNA PCR Primer, Index 36 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTC | 9 | 0.10861694424330195 | RNA PCR Primer, Index 26 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 250 | 0.0 | 42.300003 | 45 |
ATGCCGT | 265 | 0.0 | 39.905663 | 44 |
ATCTCCG | 270 | 0.0 | 38.333332 | 10 |
GCAGCAA | 270 | 0.0 | 38.333332 | 31 |
TCCGAGC | 270 | 0.0 | 38.333332 | 13 |
AGACCAG | 265 | 0.0 | 38.207546 | 25 |
GACCAGC | 265 | 0.0 | 38.207546 | 26 |
ACCAGCA | 265 | 0.0 | 38.207546 | 27 |
GTATGCC | 280 | 0.0 | 37.767857 | 42 |
ATCTCGT | 280 | 0.0 | 37.767857 | 37 |
TATGCCG | 280 | 0.0 | 37.767857 | 43 |
TCTCGTA | 280 | 0.0 | 37.767857 | 38 |
CTCGTAT | 280 | 0.0 | 37.767857 | 39 |
AGCCCAC | 275 | 0.0 | 37.636364 | 17 |
AATCTCG | 275 | 0.0 | 37.636364 | 36 |
CACATCT | 275 | 0.0 | 37.636364 | 7 |
GCCCACG | 275 | 0.0 | 37.636364 | 18 |
CCAGCAG | 275 | 0.0 | 37.636364 | 28 |
CCACGAG | 275 | 0.0 | 37.636364 | 20 |
CATCTCC | 275 | 0.0 | 37.636364 | 9 |