FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_95.35100000042b31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_95.35100000042b31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8286
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC200924.24571566497707No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT1882.2688872797489745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG570.6879073135409124No Hit
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC270.3258508327299059TruSeq Adapter, Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT130.1568911416847695RNA PCR Primer, Index 18 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTT90.10861694424330195RNA PCR Primer, Index 4 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT90.10861694424330195RNA PCR Primer, Index 36 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTC90.10861694424330195RNA PCR Primer, Index 26 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2500.042.30000345
ATGCCGT2650.039.90566344
ATCTCCG2700.038.33333210
GCAGCAA2700.038.33333231
TCCGAGC2700.038.33333213
AGACCAG2650.038.20754625
GACCAGC2650.038.20754626
ACCAGCA2650.038.20754627
GTATGCC2800.037.76785742
ATCTCGT2800.037.76785737
TATGCCG2800.037.76785743
TCTCGTA2800.037.76785738
CTCGTAT2800.037.76785739
AGCCCAC2750.037.63636417
AATCTCG2750.037.63636436
CACATCT2750.037.6363647
GCCCACG2750.037.63636418
CCAGCAG2750.037.63636428
CCACGAG2750.037.63636420
CATCTCC2750.037.6363649