FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_94.35100000042b17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_94.35100000042b17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131048
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC52053.971827116781637No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT13261.011842988828521No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG6690.510499969476833No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC1890.1442219644710335No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1780.13582809352298394No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.1343019351687931No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.1274342225749344No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA1580.12056650998107563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.11598803491850314No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC1470.11217263903302607No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11064648067883524No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA1410.10759416397045359No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1370.1045418472620719No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG1360.10377876808497649No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC1330.10148953055369024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5600.042.58928345
GCCGTCT356.206008E-638.57142645
ATGCCGT7100.033.5915544
ATCTCAT352.8068444E-432.1428576
CGAGACC7650.031.76470823
ACGAGAC7650.031.76470822
CACGAGA7700.031.55844321
GAGACCA7650.031.47058924
CCCACGA7750.031.3548419
CCACGAG7750.031.3548420
GACCAGC7550.031.29139126
ACCAGCA7600.031.08552627
AGACCAG7600.031.08552625
CGTATGC7700.030.97402841
GCAATCT7400.030.70946134
CTCGTAT7700.030.68181839
AGCAATC7500.030.633
TCGTATG7800.030.57692340
CAGCAGC7750.030.48387129
CCAGCAG7750.030.48387128
Produced by FastQC (version 0.11.2)