FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_93.35100000042afc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_93.35100000042afc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186622
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC50722.717793186226704No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT17250.9243283214197684No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5750.3081094404732561No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG5630.30167933041120554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.20844273451147238No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.20362015196493444No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.20254846695459272No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3540.18968824683049157No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.18004308173741573No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2560.13717568132374533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.12645883122032772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.12592298871515686No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1940.10395344600315076No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.10341760349797988No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1890.10127423347729636No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGCC207.0195284E-444.99812320
CCTCTTA207.0195284E-444.99812325
TGCCGTC4650.043.06271745
AGGCAGC250.002103028435.99849718
TGGCTGA501.966404E-631.507136
CGGGCAA501.970524E-631.49868616
ATGCCGT6600.030.33964544
ACAGTTC300.00513066130.006793
GGCAGCA300.00513739229.99874919
TCGTTAG300.00513739229.99874916
TAGGTTA300.00513739229.99874918
CTAGTAC300.00513739229.99874931
CTACGGG300.00513739229.99874911
ACTCCTA300.00513739229.9987497
CCAACGA300.00513739229.9987499
AGATGTC300.00513739229.99874916
GCATATG300.00513739229.99874934
TCCAATA406.148146E-428.12382724
TATGCCG7400.027.05968343
CACGAGA7500.026.99887521
Produced by FastQC (version 0.11.2)