FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_92.35100000042ac5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_92.35100000042ac5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170620
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC27171.5924276169265035No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT8540.5005274879849959No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5210.3053569335365139No Hit
AAAAAGGCTTTGGAAAATATCAACTCCCGTCTTCGTCTTGTTATGAAGAGT4410.2584691126479897No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3940.2309225178759817No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.20982299847614583No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.2063064119095065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.17641542609307231No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.17407103504864613No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG2700.15824639549876918No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2600.15238541788770368No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2460.14418004923221192No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2180.12776931192122848No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2130.12483882311569569No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2050.12015004102684328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11311686789356465No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.11018637908803189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.10784198804360567No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT1830.10725589028249911No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1810.10608369476028602No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1740.10198101043254015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGT207.0166145E-444.9997068
CGGGATA207.0166145E-444.99970617
TGCCGTC3550.041.19691545
GCCGTCT356.217342E-638.57117545
TCCCGTC601.5279511E-1037.49975625
AGATCCG250.002102160135.99976710
ATGCCGT4000.035.99976744
CCCGTCT653.6379788E-1034.6151626
CCAGCAG4500.033.4997828
GAGACCA4500.033.4997824
GCAATCT4550.033.13165334
TCTCGTA4350.033.10323338
CGAGACC4550.032.6371523
ACGAGAC4550.032.6371522
ATCTCGT4450.032.3593437
CTCGTAT4400.032.215739
CCGTCTT708.039933E-1032.14264727
CAGCAGC4700.032.0742629
GACCAGC4700.032.0742626
ACCAGCA4700.032.0742627
Produced by FastQC (version 0.11.2)