H23LNBCXX_l01n01_bir_1228_pb_89.35100000042a75.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pb_89.35100000042a75.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20739
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC	3132	15.101981773470275	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT	345	1.663532475047013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	191	0.9209701528521144	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	138	0.6654129900188052	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.5159361589276242	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG	92	0.44360866001253674	No Hit
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC	88	0.42432132696851343	TruSeq Adapter, Index 4 (95% over 22bp)
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	75	0.3616374945754376	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.2652008293553209	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC	42	0.20251699696224504	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC	42	0.20251699696224504	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.19769516370123924	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG	40	0.1928733304402334	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.1928733304402334	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	39	0.18805149717922753	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA	39	0.18805149717922753	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	38	0.1832296639182217	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	37	0.17840783065721585	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	37	0.17840783065721585	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG	37	0.17840783065721585	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	36	0.17358599739621003	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	36	0.17358599739621003	No Hit
TTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.16394233087419838	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.16394233087419838	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	33	0.15912049761319255	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	33	0.15912049761319255	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT	32	0.1542986643521867	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	30	0.14465499783017502	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	28	0.13501133130816334	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	27	0.13018949804715754	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.13018949804715754	No Hit
TATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTT	26	0.12536766478615172	RNA PCR Primer, Index 4 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	25	0.12054583152514586	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	24	0.11572399826414004	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	24	0.11572399826414004	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	295	42.69123	45
ATGCCGT	320	39.35598	44
GACCAGC	350	36.069733	26
GCAATCT	345	35.852238	34
GAGACCA	360	35.607788	24
AGCAATC	355	35.56171	33
AGACCAG	355	35.56171	25
ACCAGCA	355	35.56171	27
TATGCCG	355	35.47581	43
GTATGCC	350	35.340065	42
CAATCTC	350	35.340065	35
CGTATGC	350	35.340065	41
AATCTCG	345	35.20038	36
ATCTCGT	345	35.20038	37
TCTCGTA	345	35.20038	38
TCGTATG	345	35.20038	40
CTCGTAT	345	35.20038	39
CACGAGA	365	35.12001	21
ACGAGAC	365	35.12001	22
CGAGACC	365	35.12001	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content