FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_88.35100000042a5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_88.35100000042a5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10973
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC174415.893556912421397No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT2492.2692062334821834No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG670.6105896290895836No Hit
TTGTAAGGTGTATTCCTGTATACTACTTGAGAATACACACTTAGCTGTGCA460.41921079012120666No Hit
TAGATCTCATCCATCATTTGAACAAGAATATTGATGCTGTTCTTTTTATCG440.4009842340289802No Hit
TGATAACTATGTGAGGTGAAGGATATGTTAATTAGCTTGATTGTGGTAATC430.3918709559828671No Hit
TATCTGACTTATACAACCTCAGCATTAACTAACTCAGAACAGAAACAGAAA430.3918709559828671No Hit
TTATTCACAATAGCAAAAAGATGAAAACAACCCAACTGTCCATTAACAGAT410.3736443998906407No Hit
GGAAGATTTAATCTGAAAAGCAGAGTGCAATTCTCAATGCTAATTTTTCAA380.3463045657523011No Hit
CTATATAGATAGTGTGATGAAACAATGGAATATTCCCGGTTTAGCATTAGG350.31896473161396155No Hit
AGTTTAGATTGGTAAAAATGCAGAGCAAGAAAAAGAGAACAATAGTTTCAA340.3098514535678483No Hit
GTGTATTATATACTTGGAATTTGCTGAGAGGGTAAATCTCAAATATTCTCA320.291624897475622No Hit
AGTATGAATAAGCAGACAGTACTGCCAATACAGATATTTTAGGCAGTGAAC320.291624897475622No Hit
TCTAAAAAGGAAAAAGAAAGTTCTTTGAGGATGGAATTGACTGAACTATTT310.28251161942950875No Hit
TATCAGGGTATTGAAATGTTTACTGCTATGCGTAGATTAAACAAACCTGTA300.2733983413833956No Hit
GAATGGAATCATCGAATAAAATTGTTTGAAATCATCATCGAATGGAATCAA290.2642850633372824No Hit
GTTTCTTTTTGATTCGATTCGATTAGAATCTTATGATTCAAGTCTTTTTGA280.25517178529116924No Hit
GGATCTATACCTTCCATAAAAGCTTTGATATACAAACCCGTACCACCTACC270.246058507245056No Hit
ATCATTAATGATGTAATGCCATTCTCTCCTTTTTCATTAATATCGACTCCC260.23694522919894284No Hit
CAAATGGAAAAAATTTGTAAGGGATTAGAGTATGGACTTGATGTTTCATTT250.22783195115282967No Hit
CTTGTAAATTTATTGAAGCTCATCTTATGGCTTAACATATGGTCTATTCTG250.22783195115282967No Hit
ATACCATGTTCCATTTATTTGCTTCCATTCATTTGCAATGTAGGAATTATC240.2187186731067165No Hit
ATTTTACCTTTGATTGATAAAACTTGATAGTAGTTTCCTGAAACTTTGCTG240.2187186731067165No Hit
CAATAATGTAACCAATGGAAACTATACGACCATATAAAATCAATTTTCTGT240.2187186731067165No Hit
ATGTTATTTGGTACTCAAGTTGTAAATAACGTCTACTACTGTTTATTCCAG220.2004921170144901No Hit
GGGTAAGATTAGTAACCTGTTGTGTAGCTACTTTATAATTATGCATCACAG210.19137883896837693No Hit
GTTAGAAACAATCTACATAATGAAAAGTCACTTTTGGGGTAAGGAAATGAC200.18226556092226376No Hit
AAATGATACTTAGAGAAAAGTATCATTTCAGCAACTTCTTTACCAGTTAAT200.18226556092226376No Hit
GAACAATGTACACCATACTAGCACATCTGATTCACAAAATACTAATGCGTC200.18226556092226376No Hit
CTCATATATTGTTGTTAGAGTAAGTTGGTGTAATTTTCTGGAAAGTGATTT190.17315228287615056No Hit
TTCCATATACATCCAAATTGTCCTTCATGAAAGAAGACAGAATGAAAATAT190.17315228287615056No Hit
ACTGAATCTTCATTTGAAAAGACTAAAGCATTTGTACCTTTTAATTCGCTT190.17315228287615056No Hit
GTTTTATGTCTTCATCTTCCCTGAGAAATTTAAAATTTGCGCTTATACAAG180.16403900483003736No Hit
AAAGAATATAATCACAAAATCACTGCTGATCGATAGCTATCAAAAACTAGG180.16403900483003736No Hit
GATACAATTGAAGCAACAGCTAAATGTTTTACAAATGATTTGTCTATGAGT180.16403900483003736No Hit
TAACTAGTCTTAGTTTTGTTCTATCAGACTTGTCATATCATTTCCCTCCAA180.16403900483003736No Hit
GTATTGTAGCTATTATTCTTGTTTTATGGGGAATCAGCTACAAGTTAGAAG170.15492572678392416No Hit
CTATAAACAATTAGAAGGCAAACAAAAAATGGATAAGTATTTGCCACATAC170.15492572678392416No Hit
AATGATCATTTTGTCTGTTTTTAGATTGCAAACGACACATATTTATTGTCA170.15492572678392416No Hit
AAGAAAAAAAACAACACACGTTTTCAACTTGGGCAAGTCAATTGTATTTCA160.145812448737811No Hit
ATATTGTATTAGCCTACATCTACTCAAAAAATGCACCAACACCTATCACTA160.145812448737811No Hit
GATTTAATTCGATTCTATTCATTTCGATTCGATTCGATTCGAATTTTTCGA160.145812448737811No Hit
TAGATAAACCACTCTACAAATATCTAAGCAAACCAATTTCCGAGTATGAAT150.1366991706916978No Hit
AACAGATTGAGGAAGTTCTCTTCTGTTCCTAGTTTGTAGAGAGATTTTTAT150.1366991706916978No Hit
GAATCTACGTTGCAGATTAATAATTCAGGGTTTAAGATTTGATAAGCAGAA150.1366991706916978No Hit
GAGTTAAAGACTTAAACATATCTTTTTTGGAAGGGAACACAACTCAACCTG150.1366991706916978No Hit
ACGTTTGTATACTTTAACAGCATCTGCAATGTCTACAGTATCTGCTTCAAT150.1366991706916978No Hit
CTCATGAATAAATCAACAAAAATCTTAAACAACAACAACAACAACAAAAAC150.1366991706916978No Hit
CTCTTTAGTTTAATCTGTGATCTGAAAACTTCCTCAGTCTTTATGTTGATT140.12758589264558462No Hit
AAAACTAGACAGAAGCATTCTCAGAAACTTGTTTGTGATGTGTGTATTCAA140.12758589264558462No Hit
CAATTTTGTTAATCCTTTACTAAAACACATGTCAAAGTTCGATTTTTAGTG140.12758589264558462No Hit
TTGAAGAGTTAACCAACAATTGGAACTAATCAATGATTTCAGAAAAGTAGC140.12758589264558462No Hit
ATAGTACTTCGTTTCCAATGTATTTCCATTTGCTGATTTAAAATTTAATCC140.12758589264558462No Hit
ATTTTGATGATTTGTCTTACACAATGGCAAGATGTGTTAGATTTGTAACAT130.11847261459947142No Hit
GATAATTGTCTTTGTCTGATTGACTTATTTCACTTAGCATATTATCCTCCA130.11847261459947142No Hit
ACTATGCACAAGAATATGAATTAAACGACGCATTAGAAATTGTTGAAAAAT130.11847261459947142No Hit
ATCTTGCAGATACTTTTTGTATGCATTTTTGTTAGATTTCTCTTTTTTGCT130.11847261459947142No Hit
CTTTAAAACTAGAAGTTGAGAAAAGTGCATTCTTAAACTTGTAAGTTTGAC130.11847261459947142No Hit
ATTATTGCGAGAGGGTAAAACAAAATTAAACGCAACTTTTACTGTTGATAC130.11847261459947142No Hit
GTATTACCGAAGAAATCAATGCGGGTTATTTTGAACAGTACGCTTTACAAA130.11847261459947142No Hit
CAGTATATCCTCTTCAGAATCTGTTGTTTCAATACCAACCAATACTAATAA130.11847261459947142No Hit
CAAGAAACTAATGCTATTGCTATCCTTATTCAAGAAACTGATGAAGATTAT130.11847261459947142No Hit
ATATAAAGGAATTAGCAAGGGAAACTGAGGTAATATTCTTACGATGTGTTG120.10935933655335825No Hit
TATTTGAAGGATAACTTTGCTAGATACATTGTTTTGGGTGACAAATTTATT120.10935933655335825No Hit
ATACTGTTGCATTCAGGATTAAGTTTCAACATGAATTTTGGAGAGGGCATA120.10935933655335825No Hit
ATATATGCAGTAAGAGTGGAAATCAGATGATAAAATCTACCAGACTACTAG120.10935933655335825No Hit
GTTTTAAATACATGACCTTTGCTGAATATGAGATTTGCCAATATTTTCTAC120.10935933655335825No Hit
GGAATGGAAGGAATGTTTTTAAAAACTTAATGAAGCCACTGTCAATCTAAA120.10935933655335825No Hit
ATTTTGAGTAAACGACTTTTAAAATCTAGCATTGTTGTTAGTTCTATGACT120.10935933655335825No Hit
ATTATTGGGAATCCAAATGAAATATTTGGAACAAAGTTTCCATCCATTACA120.10935933655335825No Hit
GGAGTAAACTAAAAGATGAAGGAACGATATTTAACTAAAAGTGCGGTTTTT120.10935933655335825No Hit
ACTTAGAGGTAAAGGAACATACAAAACATGTTACAAATCTCCATGTTCTAA120.10935933655335825No Hit
ATGAAGAAGACAGAATATTATGGCAAAATGCTGGAAGTATTGAAACTTGAG110.10024605850724505No Hit
GATCCTAACAGGTGTTTTTGGAAACTAGATAAATGGATTCAAAAATTCATG110.10024605850724505No Hit
TCTAAAACTCATACGAATCAAAATGATGATGTAAATAGCAGATTATGGAAG110.10024605850724505No Hit
ATAAAAGTGTGTCACTTGAGGATTGTTGTATTGACAGAACTGTTTTGTATC110.10024605850724505No Hit
ATCTAACTTATAAGATAAGGCAAAAAAAACCTAGTCATTTGACTAGGTTTG110.10024605850724505No Hit
AGTCTACTTCAAGAGATGGTAACAGTAAAACCACCTTCAAGCAAAAAAGGT110.10024605850724505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1850.043.75712645
ATGCCGT2100.038.54794344
AGCCCAC2200.036.7957717
ATCTCCG2200.036.7957710
ACGTCTT2200.036.7957727
CCACGAG2200.036.7957720
CATCTCC2200.036.795779
GAGCCCA2200.036.7957716
CACGAGA2200.036.7957721
ACATCTC2200.036.795778
AGACGTC2200.036.7957725
ACGAGAC2200.036.7957722
GACGTCT2200.036.7957726
GTCTTAG2200.036.7957729
CGAGCCC2200.036.7957715
CCGAGCC2200.036.7957714
TCTCCGA2200.036.7957711
CGAGACG2200.036.7957723
TCCGAGC2200.036.7957713
CGTCTTA2200.036.7957728
Produced by FastQC (version 0.11.2)