FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_87.35100000042a25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_87.35100000042a25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116328
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC41133.535692180730349No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG21461.8447837150127224No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT13301.1433188914104946No Hit
GTATAAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT5740.49343236366137133No Hit
GTAGTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTT4770.4100474520321849No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC3710.3189257960250327No Hit
GTTATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTAT3330.2862595419847328No Hit
GTATATATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2940.25273364968021456No Hit
CTTTAATATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2130.1831029502785228No Hit
CTTTCTATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT2060.17708548242899386No Hit
CTTCTAATACGTTTTGGTAAAGAAGAGAGTATAAAGACTGTCTCTTATACA1980.17020837631524655No Hit
GTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1810.15559452582353345No Hit
TAATAGTACACAGTATTAGCTGTCTCTTATACACATCTCCGAGCCCACGAG1650.1418403135960388No Hit
ATATATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTA1620.13926139880338353No Hit
GCATAAATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1500.12894573963276254No Hit
GAATTAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1490.12808610136854412No Hit
CTATATTACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTA1470.12636682484010728No Hit
TTGTAGTATAGTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTC1320.11347225087683104No Hit
GTACTATATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT1200.10315659170621003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4000.040.49535845
AACCGCA250.002100529135.99587232
ACCGCAA250.002100529135.99587233
GTAGTAT1000.031.5234931
ATGCCGT5300.030.56253244
ATAATCC300.00510979830.0223753
GTTCAAG300.005131353629.99656311
CCGCAAA300.005131353629.99656334
TTCTGTG406.136721E-428.1217757
TAAATAC654.77894E-727.7129634
CTGTGTA608.116347E-626.2469929
TGTATAA957.185008E-1026.0720632
CTGTAAA450.001214638825.0186464
AACTGTA450.001214638825.0186462
GTTATTT901.15851435E-825.0186461
GGTCAAA450.001220751624.99713311
TAATATC651.4998073E-524.2488424
GTATTAG651.4998073E-524.2488421
TCTGTGT651.5098474E-524.2279918
TTAATAT751.6650174E-624.01793
Produced by FastQC (version 0.11.2)