FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_86.35100000042a0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_86.35100000042a0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320418
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC78082.436816907914037No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG9880.3083472214419914No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT9000.28088309645525533No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8250.2574761717506507No Hit
GATATACACATTCTGATGTTGTTTATAAAAGGCCAATTAGGGATTTAGATA8180.25529152544488765No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.18663121297804744No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5890.18382238201349488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.16790567321436373No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4320.13482388629852254No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3970.12390065476970706No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.12171600846394397No Hit
ATTAGATACAACGCTAAGAGAAGACACTGGAAGAGAACTAAGTTAGGTATC3600.11235323858210212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.10892022295876011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.10704766898239175No Hit
GATTTAAGACTGGTAACACAATTAGATACAACGCTAAGAGAAGACACTGGA3270.10205419171207611No Hit
TTCTGATGTTGTTTATAAAAGGCCAATTAGGGATTTAGATACCTAACTTAG3220.10049373006510245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8800.041.41801545
ATGCCGT9400.038.53496644
CGGATGC250.00210545535.9978823
AGAGCCC250.00210545535.9978832
TATGCCG10600.034.17251643
CTCGTAT10650.034.0120839
GTATGCC10650.034.0120842
ATCTCGT10750.033.90497637
TATCTCG10850.033.5924936
CGTATGC11000.032.92987441
TTAGTAT10950.032.8747732
CATGCGT551.12397174E-732.7253410
TCGTATG11100.032.6332140
TCTCGTA11150.032.48687438
ACTTCGA352.8168416E-432.1409635
AGACGTC11150.031.88152925
GTATCTC11300.031.8565335
CGTCTTA11200.031.739228
GTCTTAG11200.031.739229
ACGTCTT11250.031.59813727
Produced by FastQC (version 0.11.2)