FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_85.351000000429fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_85.351000000429fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243174
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC145345.9767902818557905No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG58982.4254237706333734No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT31641.301125942740589No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9110.37462886657290667No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA8900.36599307491754873No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG7050.2899158627155864No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA6580.2705881385345473No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC4940.20314671798794282No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4630.19039864459193828No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4230.17394951762935182No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA4220.17353828945528715No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA3920.1612014442333473No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3750.15421056527424806No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3650.15009828353360144No Hit
GTGCATTTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGT3550.14598600179295484No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA3430.1410512637041789No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG3260.13406038474507964No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3200.13159301570069168No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA3190.13118178752662704No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3090.1270695057859804No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC3000.12336845221939845No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA2930.12048985500094582No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC2910.1196673986528165No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2880.11843371413062251No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2880.11843371413062251No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG2800.11514388873810522No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT2780.1143214323899759No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2770.11391020421591125No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2560.10527441256055335No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG2540.10445195621242402No Hit
GTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCAC2500.10280704351616538No Hit
GTGTTGGGTTGACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2480.10198458716803606No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT2460.10116213081990673No Hit
GCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCG2450.10075090264584208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCC253.881706E-545.00000410
TTAGCGT253.881706E-545.0000043
TTTTAGC253.881706E-545.0000041
TGCCGTC17100.043.42105545
TTTAGCG301.13727685E-437.4999962
GGGTGAC301.13727685E-437.4999966
CAGCCCC301.13727685E-437.49999612
ACAGCCC301.13727685E-437.49999611
TACAGGG301.13727685E-437.4999962
ATGCCGT20200.036.75742744
CAGTTCG250.002103849536.0000049
GCACGTC250.002103849536.00000433
GATGCGT401.5555306E-533.7515
TAGCGTT352.8138826E-432.142864
TTAATAG453.4796532E-530.03
GCAAATA300.005139373729.99999823
AGGAATG300.005139373729.9999982
GCACATA300.005139373729.99999831
TAATAGT406.152057E-428.1254
CGTGGAA406.152057E-428.12519
Produced by FastQC (version 0.11.2)