FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_84.351000000429d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_84.351000000429d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237852
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC65902.7706304760943783No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG17400.731547348771505No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT7490.3149017035803777No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3860.1622857911642534No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC2920.1227654171501606No Hit
ATCTTACAGCATGTGTGTTGGAAGATGAGAAGTTAAAACTGAGTTATAAGA2650.11141382035887862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.1051073776970553No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2430.10216437112153777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6850.042.3718745
ATGCCGT7450.038.9593744
GATCGTA250.002103854435.9996625
CGTATGG250.002103854435.9996628
TAGCTTC504.8516085E-835.999669
CTCTAAT401.5510139E-533.763881
TATGCCG8900.032.61205743
GTATGCC9150.031.96691342
CGTATGC9300.031.20938741
TCGTATG9400.030.63801240
CTCGTAT9450.030.47590439
TCTCGTA9550.030.15678838
TCTCTCC300.005139385429.999723
GTAAGAC300.005139385429.9997212
ATCTCGT9850.029.23830637
TATCTCG9900.028.86336736
GTATCTC10050.028.43257135
TAGTATC10300.028.17934633
TATCAGC406.151994E-428.12473719
ACCGGCG406.151994E-428.1247378