H23LNBCXX_l01n01_bir_1228_pb_8.35100000042049.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pb_8.35100000042049.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49869
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	5013	10.052337123262948	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT	857	1.7185024764883996	No Hit
ATCTTCATCAGAGTCTTGAAGAATTTGTTCTTTTGTTGAAATTCCTGCAAT	490	0.9825743447833324	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG	208	0.4170927830917002	No Hit
GTTATACTAACAGCTACAACTACTGGTAATGGAACATGTAGTCCTATTGTT	193	0.3870139766187411	No Hit
AAATGGATCTTCAACAAATGTTTCTTCTGTTAACTGGCAGATATTATCTGG	189	0.3789929615592853	No Hit
AAATCATCCTGATAATATAGCACCTGTAAGCAACCAAGATATTTGGTATGC	173	0.34690890132146224	No Hit
GACACTAATGGACTTAATGAGTTTGTCACATAACAGTCAAATGTCAGTATT	164	0.3288616174376867	No Hit
TAAGAGATTCTGCAACGATAAGAAAAATGTTCCATATATTGCTGTGGAAAC	163	0.3268563636728228	No Hit
GTATTTCCATATAATCGCTCAACAACTTAGTATGCTTTTGACGAACATCTT	161	0.3228458561430949	No Hit
TATGATTTCCTTTTTGTTGACGGAGTTTTCAAATGCTGTGTTCAAATATCA	141	0.28274078084581605	No Hit
TCACTAAGCAATGTGTTAATCCGTTGTTCTATTAATGCTTTTGAAGTACCC	136	0.2727145120214963	No Hit
ATGTACAACAGGTAACAGATTAAAAGGATCAGCACTAAAAAAAACCACATC	135	0.2707092582566324	No Hit
TGTTATTTTGATGATGGCCTTTTATTCTTCTCTTTTAACTTCCATTGATGG	132	0.26469349696204053	No Hit
TTTGTTTTACCATAGTCCTTGATGGTTTTTTTTAATTGTTGCTGGTAACTT	129	0.25867773566744867	No Hit
AAATACCCTGATAAGAAAGGTTATTCCACTCGTAACCTTATCTATATGTGC	127	0.2546672281377208	No Hit
AAATAAGTTGATTTGCAGTTTGAAGGATAAAACAACAAAACTACTGTTTTA	118	0.23661994425394534	No Hit
GTTTTAGATGCTGATACTTATGTTGATTGGACGGATAGTTTATTGAAAAAA	112	0.22458842166476167	No Hit
GTTTTAACTCATTTTCAATCGTTCTTAGCAATTCAAAGGTTATCATGAAGC	109	0.2185726603701698	No Hit
CATAAGCATTGGGAAAATTTAGAAAAACTTCGTAAAGCAGAATGGTTGGAA	106	0.21255689907557804	No Hit
GTTTTATTTCCAAATGGGTTGTTTTAGGAACTATAGTACTTGGACTGCTAG	105	0.21055164531071408	No Hit
GATATCTACATAAACAATATCTCCCAATTCATGCTGTGCAAAATCGGTAAT	101	0.20253063025125828	No Hit
AAACTATTTTGGTGATACTCCTTTAAAGCCATTTAAAGATGCGGATGATCT	100	0.20052537648639437	No Hit
ACATTATTTCTGCAGTGGGGTTTTTAAAATCTTACTACAGTCACAGGGAAA	99	0.1985201227215304	No Hit
TTCATGTTTTACAACATGAAGATAGAACCGAAATAGGTAACTACATTTTCT	91	0.18247809260261888	No Hit
GAGTTAATACACTACGGCTGATTTTTACTACGTGATTAAGCTCTATAAGTA	90	0.18047283883775492	No Hit
ATTTCATATAGTGTCGTTTCGACATTGGGAATATTTACATCTGCTCCGATT	84	0.16844131624857125	No Hit
CTTTTCTATTGATCAAAGAGGAAAATATGTGAACGCTAAGGAAATGAATCA	82	0.16443080871884336	No Hit
ATTATGATGAATTTGCAGCTTTTACTTAGTAACTTCATGGAATTCACAAAA	82	0.16443080871884336	No Hit
AGATACAAATAACAATGAAGCAGCTTACAATATTCTCAGCAATAGTAACTG	75	0.15039403236479576	No Hit
AAGTTTTGTTTTGAATGTGTTTTGCAAAGATTATTTCATCCTTAATGTCCT	74	0.14838877859993183	No Hit
GACATATACTGCTCCAACAAAAATATGTATCTGAAATAGCCTCACCATTTA	74	0.14838877859993183	No Hit
ATGATATTCAGTATGATGCGATAAAGAAGGCTTTGGGAAAATAGTTTCTAG	71	0.14237301730534	No Hit
AAGATGAATTTAGTGAAGACCTCAAAGATGAATTTGAAAATTCAAACGATG	71	0.14237301730534	No Hit
ATACTTTTAAGGACATAATCATGGTTTTCTACTTTTCTGTGGAGCCTGAAA	71	0.14237301730534	No Hit
CAGATATATCCTCAATTTCTTTACGAACTCTACTCTTAGTCTTCTGCCAAT	70	0.14036776354047603	No Hit
GGAATTACACTGAAGGAACATATTTTTCAGTTATGACAGTCTCAGTCACTG	69	0.1383625097756121	No Hit
ATCTACCATTGTAGTTTCGTTATTGGGTGCATTTGTAGAATGGTTAAATCT	68	0.13635725601074816	No Hit
TGTATCAACACCAAGAATAGAGTTGCAAATTAAGTGATCTGAAATACGAGA	68	0.13635725601074816	No Hit
GAAAGAAACTTAACCCTTCATTTTCCATAGAAGTAACCATTTGGTCTTGAA	68	0.13635725601074816	No Hit
ATGGATATGTTTGATTAATACAGCTTTGAATGGCTTGTGATAGATAATGCG	68	0.13635725601074816	No Hit
AATCTAATTCGTACCTGAATGTCAAGAACATTTCATATAAACTTGCACACA	67	0.13435200224588423	No Hit
AACTAAGATTGCACAATTGTCATTTGTCTCTTCTGTTGAACCTGTATCAGT	66	0.13234674848102027	No Hit
TATTAGAAGAAAGATCCATGAAAATCCAGAATTAGCTGGCAAAGAAGATGA	62	0.1243257334215645	No Hit
ATCTAAGAATTGGTTTGCAGAATATGTGAACTACCACAACTCAATAATCAA	62	0.1243257334215645	No Hit
ATTTAGAAAAGAAAATGCAGAACTTGGAAAAGCAAACAACTCTTTCATTAA	60	0.12031522589183662	No Hit
TGTTTGTATATCAAGCTCTTTGTAGATTTCACACTAACCGTCTTGGATACT	60	0.12031522589183662	No Hit
AATCGGAGTAATCTGAATGATTCCTTTTTCTAAATCGACTACTGGGATAAA	59	0.11830997212697267	No Hit
ATAGAATACTTTCTAACGCAGAAGTAAACCTTTCTTTTGGTGATCAGGTAC	58	0.11630471836210872	No Hit
TGACAAAATATTGATGATTGATGCAAGGAATGTTTTCAGAAAGGTTACTAC	58	0.11630471836210872	No Hit
ATGATATTTTCCGTTCTCAGGTATTTGAGCAATATTCTGAAAGTAGCTTCT	58	0.11630471836210872	No Hit
ATCCATACTAATGCTAGTGTAGATACGATACTTGTGTATAGCAAGAAAATG	58	0.11630471836210872	No Hit
ATAAGGAATCACCCTAATTGTATATGTACCACAAGCAAATTCATTCATAAC	58	0.11630471836210872	No Hit
TGTACAAAATCTAGAGCAAAAGTTTGGAAATGAACTAAACATTTAGTCTAG	58	0.11630471836210872	No Hit
ACCATATACTTACACTTGTGTCTTACTTCTTTAGGAGGGAAGATCTTTCAT	56	0.11229421083238084	No Hit
AGACAAGAAGACAAACTGTTTACAGATATTATTAGGCCTTTTCAAAAATGC	55	0.11028895706751689	No Hit
GTGTTTATACTGTTTCTGTAAATACGGGTAGCGATGGAAATACGGTGTTAG	55	0.11028895706751689	No Hit
ATCTTGGTTACTTGAGGTATTTTCTTCTTTAATTGGCTTTTACGATCAACA	55	0.11028895706751689	No Hit
GTAGAAGAACTCGATAAAAAAACATTGGTTATTCGAGATGTACCTTATAGT	54	0.10828370330265295	No Hit
GAGTAAGAATCTATTCCCTGGAATATACATCCGTAACTGAAGTCTCTTCTT	52	0.10427319577292506	No Hit
ATAATACATAACCAGCTTGGAATCGCAAAAATGATAAAGAAGGCATCATTT	52	0.10427319577292506	No Hit
CTTCTTACTAAACTGACTTTGTTTTTTCTTGTTTTTTCTGAGCGGGAAATA	52	0.10427319577292506	No Hit
AATTGGGATAGCAGTTAAAGAGGTATCTAGAATAACACCTTGATACCAAGA	52	0.10427319577292506	No Hit
ATCCATTTCATTCCATTCCATTCCATTATATTCCATTCCATTCCATTCCAC	51	0.10226794200806112	No Hit
ATAAATATCCCATCATTTAGAGCTGATAAAAAGGAACAACCAAAGCCAATA	51	0.10226794200806112	No Hit
GATAGATATGGATAAGAATTGGATTCCTAATATTGCTGATCACTCTTTGTA	50	0.10026268824319719	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAA	25	3.82804E-5	45.018074	26
AAATCGA	20	6.954632E-4	45.018074	32
CTCGTAA	25	3.82804E-5	45.018074	29
CATGCTG	20	6.954632E-4	45.018074	30
TCTTCTC	20	6.954632E-4	45.018074	26
ATAGCAC	25	3.82804E-5	45.018074	17
ACTTCGT	20	6.954632E-4	45.018074	27
AAGGTTA	25	3.82804E-5	45.018074	17
ACCCTGA	25	3.82804E-5	45.018074	5
TGGCCTT	20	6.954632E-4	45.018074	15
TGTGCAA	20	6.954632E-4	45.018074	35
AGAACCG	25	3.82804E-5	45.018074	24
ATTCCAC	25	3.82804E-5	45.018074	23
CCCAATT	20	6.954632E-4	45.018074	23
CCACTCG	25	3.82804E-5	45.018074	26
TCGGAGT	20	6.954632E-4	45.018074	3
CACTCGT	25	3.82804E-5	45.018074	27
TATCTCC	20	6.954632E-4	45.018074	18
TCAGCAC	25	3.82804E-5	45.018074	29
GGAGTAA	20	6.954632E-4	45.018074	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content