FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_79.35100000042933.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_79.35100000042933.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227385
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC50262.2103480880445057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG25511.1218857884205202No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT10870.4780438463399081No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGC5150.22648811487125362No Hit
ATATAAGATCATCTCTTACTTTACAAACAACAATGGCTGTCTCTTATACAC2770.12181982100842184No Hit
GAACATCTTTATTTCTGCCTTCATTTCATTATGTACCCAGTAGTCACTGTC2740.12050047276645337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATGG207.023536E-444.997148
TGCCGTC5550.042.56486545
GCACGAT301.1374691E-437.4976211
ATGCCGT6650.035.5240644
GGACATC501.973367E-631.4988
ATAGGGT300.005129225630.0112956
CGAATGT300.00514027529.99809522
TAGTGCG300.00514027529.9980959
GCCGTCT300.00514027529.99809545
AGATCAT803.3505785E-928.1355886
CAGCTTA406.1531016E-428.1232139
CTCTTAC755.6008503E-826.99828513
TATGCCG8950.026.1435943
CTTACTT957.348717E-1026.05097615
GTATGCC9150.025.81803142
TATATGC450.00122084625.0094133
TACCTTA450.00122084625.0094132
TCTTACT901.1810698E-824.99841314
CGTATGC9600.024.13909141
GATCATC851.880926E-723.8220167
Produced by FastQC (version 0.11.2)