FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_78.3510000004290c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_78.3510000004290c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312521
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC83562.6737403246501836No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT12780.4089325197346738No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG6210.19870664691332743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.1526297432812515No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4190.13407099042944315No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.1279913989779887No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3930.1257515494958739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.10335305467472586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9200.044.26394345
ATGCCGT10750.038.09099244
CTAGATG301.1383783E-437.49799737
TATGCCG11300.036.03790343
GTATGCC11350.035.68091642
CGTATGC11600.034.91193441
CTCGTAT11700.034.61353739
TCTCGTA11850.034.36525738
ATCTCGT11850.034.17539637
AATCTCG11800.034.12953636
TCGTATG11950.034.0776840
CAATCTC12100.033.28334835
TGATAGC352.8139472E-432.14628625
CTCACTA352.8139472E-432.14628633
ATACCTA352.8139472E-432.1462866
TACAATC12850.031.52087433
CGAGACT13150.031.13902323
CTGATTA13150.030.97288528
ACAATCT13150.030.62574434
TGATTAC13250.030.56929829