Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pb_76.351000000428c7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 225755 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 36 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC | 4297 | 1.9033908440566099 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT | 660 | 0.29235232885207413 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG | 422 | 0.18692830723572015 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 400 | 0.17718322960731767 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 359 | 0.1590219485725676 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 270 | 0.11959867998493942 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 240 | 0.1063099377643906 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 235 | 0.10409514739429912 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 355 | 0.0 | 43.72099 | 45 |
ATGCCGT | 410 | 0.0 | 37.855976 | 44 |
TAGACTC | 25 | 0.0021062267 | 35.99061 | 9 |
TAGGCTC | 25 | 0.0021062267 | 35.99061 | 10 |
AATAGGC | 25 | 0.0021062267 | 35.99061 | 8 |
TATGCCG | 455 | 0.0 | 34.111977 | 43 |
CGTATGC | 460 | 0.0 | 33.741196 | 41 |
GTATGCC | 475 | 0.0 | 33.149246 | 42 |
ATCTCGT | 475 | 0.0 | 32.675686 | 37 |
CTCGTAT | 475 | 0.0 | 32.675686 | 39 |
TCTCGTA | 475 | 0.0 | 32.675686 | 38 |
CAATCTC | 495 | 0.0 | 31.823978 | 35 |
AATCTCG | 500 | 0.0 | 31.041899 | 36 |
TCGTATG | 520 | 0.0 | 30.28056 | 40 |
GCATTCG | 30 | 0.005128461 | 30.012117 | 22 |
GACTGAT | 525 | 0.0 | 30.012117 | 26 |
ATAGGCT | 30 | 0.005145162 | 29.992176 | 9 |
GTGGGTA | 30 | 0.005145162 | 29.992176 | 11 |
GCTCATA | 30 | 0.005145162 | 29.992176 | 13 |
CCACGAG | 540 | 0.0 | 29.588722 | 20 |