FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_76.351000000428c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_76.351000000428c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225755
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC42971.9033908440566099No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT6600.29235232885207413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG4220.18692830723572015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.17718322960731767No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3590.1590219485725676No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2700.11959867998493942No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.1063099377643906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.10409514739429912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3550.043.7209945
ATGCCGT4100.037.85597644
TAGACTC250.002106226735.990619
TAGGCTC250.002106226735.9906110
AATAGGC250.002106226735.990618
TATGCCG4550.034.11197743
CGTATGC4600.033.74119641
GTATGCC4750.033.14924642
ATCTCGT4750.032.67568637
CTCGTAT4750.032.67568639
TCTCGTA4750.032.67568638
CAATCTC4950.031.82397835
AATCTCG5000.031.04189936
TCGTATG5200.030.2805640
GCATTCG300.00512846130.01211722
GACTGAT5250.030.01211726
ATAGGCT300.00514516229.9921769
GTGGGTA300.00514516229.99217611
GCTCATA300.00514516229.99217613
CCACGAG5400.029.58872220