FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_72.35100000042841.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_72.35100000042841.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78450
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC56977.261950286806883No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT27753.5372848948374758No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG5860.7469725940089229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT253.8578757E-544.99936722
CGTGAGA253.8578757E-544.99936726
CCGTGAG253.8578757E-544.99936725
TCCGTGA253.8578757E-544.99936724
GATGGAC206.9936126E-444.99936325
GCCGTCT206.9936126E-444.99936345
AAATCCG302.139861E-644.9993621
TGCCGTC6000.043.1243945
GTGAGAT301.1303494E-437.49946627
TGAGATC301.1303494E-437.49946628
AGGTGAC301.1303494E-437.49946628
GAGATCC301.1303494E-437.49946629
CTATGTA250.002088779836.0224531
ATCAAGA250.00209533435.99949312
CAAGCCA250.00209533435.99949336
GATGAGC250.00209533435.99949324
AACTAGA250.00209533435.9994932
TACAAGG250.00209533435.99949334
ACTAGAT401.542331E-533.749523
ATCCGTG352.7968772E-432.142423
Produced by FastQC (version 0.11.2)