FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_71.35100000042827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_71.35100000042827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176907
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC4415224.95774616041197No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT78424.432837592633417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG32931.8614300169015356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGA253.877677E-544.99943511
TGCCGTC46550.044.61275545
ATGCCGT54700.037.96570244
GGTATGC250.002102432335.9995541
TATACTG250.002102432335.999555
TATGCCG58150.035.59714543
GTATGCC58300.035.50555842
CGTATGC58000.035.49524341
CTCGTAT57900.035.4788339
TATCTCG58100.035.39542436
ATCTCGT58000.035.37886437
TCGTATG58100.035.35669740
ACGAGAC58900.035.3348722
TCTCGTA58150.035.32629838
GTATCTC58050.035.30963535
CGAGACT58700.035.3019423
ACTGTAT58450.035.29895432
GAGACTC58850.035.28842524
GACTCAA58850.035.28842526
CTGTATC58450.035.26046433
Produced by FastQC (version 0.11.2)