FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_70.3510000004281a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_70.3510000004281a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311826
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC197436.331415597159955No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT63902.0492197571722692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG9910.3178054427789857No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5440.1744562672772636No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5050.1619492922334892No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3660.11737315041080604No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3590.11512830873628241No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGGA207.025494E-444.9998419
TGCCGTC20900.044.67687245
TCGTCAC250.00210479635.9998732
AGGGCTC250.00210479635.9998710
CCCGGAC250.00210479635.9998720
ATGCCGT27800.033.66894544
GTTCCCG352.815755E-432.14274217
GTATGCC29150.032.1096642
ATCTCGT28950.032.0983337
TATGCCG29200.032.0546843
CACGAGA29350.031.89085621
CTCGTAT29150.031.87810339
GTATCTC28950.031.86517135
CGAGACT29100.031.77823823
TCTCGTA29300.031.71490538
CCACGAG29450.031.70617120
ACGAGAC29650.031.56818422
TATCTCG29450.031.5533736
AGACTCA29150.031.49216825
GAGACTC29400.031.4539724