FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_7.3510000004202f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_7.3510000004202f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152259
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC93426.135597895690895No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT15060.989104092368924No Hit
ATACTTAGTGGATAAAACATTAGAGGAAAAACGAAACTTTTGTTCAGTGTT8200.5385560131092415No Hit
CATTTATCATCAATATTTTCCGGATTGATTTCAGAAGGTAGCTTTATTGAC7980.524106949342896No Hit
GAATAGTATTCCATTGTATGTATGTGTGACATTTTGTTTACCGCACTTTCT7740.5083443343250645No Hit
ATGTATCAGATTTCGAATAAGGATACCTTGTTTTTGAAAGCAGATACGCTT7580.4978359243131769No Hit
GTTATTACGTTTCTATTTGCACCTTCTTGAAAATTTCATCATTTAAGTGCA7520.493895270558719No Hit
CTTAAATCCTTTCTTTAAAGCCGTATTAACTAGTCTTGTTGCATCAAAAAA6690.4393828936220519No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6480.42559060548144934No Hit
GAAAGAAAAGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGAAAGAAAAAA6450.4236202786042204No Hit
GTTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAATGTTCTG6180.40588733670916005No Hit
ATTCTATTTGAATAAGCAGGTTTTAAAAACTCTAGCCTAAAACAGGAACCT6150.4039170098319311No Hit
ACTTAGAATGAATTAAGTTTCATTTGGTTTTCTCTACTCTCAACTGTGACC6060.39800602920024436No Hit
TCTTTAAGATGACCATTATGGATCCTTTGTCAGTAATCTTATAGATCTCCA5920.38881117043984265No Hit
GGATAAATACTAAAATGTCTACACTTGGGCATATGATAGGAACATTGAAGA5170.33955299850911935No Hit
ATCAAAAAGAATTGAATCAAATGGAATTGAAAAGAATCGAATTGAATCAAA5140.33758267163189043No Hit
GTATATATTTCCTCACCGGCTGTTTAGTATTTCATCTGCATTCCACTTTCT5060.33232846662594656No Hit
GTATGAATGGTAACTGTAATAAGTTTTAAAGTACGCTCAATAACAATTTTA4740.3113116466021713No Hit
CAATAAACAATGAGTAACATTAATATGTTCGATATTTCCAGCAACAAATTA4720.30999809535068534No Hit
GTATTTGTCAATGATAATAAAGTGACCATAGAAAACAGAGACACATTCAAC4560.2994896853387977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.290951602204139No Hit
CAATGTAGCTATAGTGTGATTTACAATAACTTTGTGTTTTTGTTATCAGCA4320.2837270703209663No Hit
ATTACTGGAATAACAACACTAAGATTTTTTCATTTCTGATCTTCATACTTT4290.2817567434437373No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.2738754359348216No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4100.26927800655462075No Hit
ACTAATCACCATAGGATCTTGCCATTTGATATTAACGTAGCTATTATTGAC4000.262710250297191No Hit
CTTCCTAAATAGCAAACATGAAATTGTCCAAACATGATAGTTTAGAGAGCA3850.2528586159110463No Hit
AAATTACTTTTAGCTCTTCAAATTTTAGCCATAATCATAATAGTTGCATCT3850.2528586159110463No Hit
TTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAATGTTCTGT3810.2502315134080744No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3770.2476044109051025No Hit
CTATTAATATAGGCCATTAGTGCTATAGTATTGTAGATATCACCGTTGATA3570.23446889839024293No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.23249857151301398No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG3540.23249857151301398No Hit
TGTGTAAATCATTCTAAGTGATGCAGTAAATTAGTGAACCTACTTCCTAAT3510.23052824463578508No Hit
GTCTATGTCCCTAATAAATTCACATTGCCAATGTCAGTGATGATAATTTCA3380.22199016150112635No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.2187062833724115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.21279530274072467No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3080.20228689272883704No Hit
GTATTGTATTTGCCAACAAATTTAATTTTCTGTTTATGGTTTTCAGGAAAA3070.20163011710309406No Hit
ATATTATGGGTTCATACCAAAATAGAACATAGAGCAGTTTCTCAATTTTTC3070.20163011710309406No Hit
GTAATAATCTCCACCTGTTTCATAGCTCAAGAAATAGCATCTTTATTTAGC3060.20097334147735108No Hit
CTTATTGAATACTGCACTGAAAATGAAAAATAGAATGTTTGTATGGATACT3010.1976894633486362No Hit
ACATTATTGGAGCAAATAATCTTAAAATAGCTTGCCATAGTCCTTTAAATA2970.1950623608456643No Hit
CAACACAAAGGATTATTAAAGGCAATTAAGGTTGAAAAATTTGTCGTAAAC2940.19309203396843536No Hit
CATGTAAAACTATGTAGTGTTTTACTAGCAAGTACAAAGGGTGTATTGTCT2910.19112170709120643No Hit
ACTATATAAAGGCAGCATCTTTTAAAAAGTGAAAACATCACCAAAAGATTT2800.18389717520803367No Hit
CTTATTAAGCCCTTTATTAAACCGGAAAGTGATTTTTACGGATCGGGATAT2790.18324039958229071No Hit
GAAACATGTTGTAATTCTTGTCCTTTTTTTTACTCTCTGTATTCTTTCAAA2780.18258362395654773No Hit
ATGCATTTCAACTAATGTATATGGAACCATTTAACAAATATCTCAAGGTAC2760.18127007270506176No Hit
CATTATTATAATTCAAGAGCATAAGCTTCAGCTCTTCTTTCAAAATTTGTA2720.17864297020208986No Hit
ATATCAGGATATTCAGAAGCCCATAATAATAAAGAGTTCTCAGTGTACTAG2680.17601586769911795No Hit
ACTTAATAGTCAAAGACACGATAGTTCTATATTGTATACATGCAATTTTGC2680.17601586769911795No Hit
ATTAAAAGGAAAAATCCAATTAACAGAAGAAAACCAAGAAATAGTCACTAC2670.17535909207337497No Hit
GGTAAAAGCAATTTGAAATTCATGATTTGAGATTTGAGATTGTAACGATTT2670.17535909207337497No Hit
ATTTAAAATGAAGCTAAAATTCAGCCCTATAGGAACAAAGAGACATAGAGA2650.17404554082188903No Hit
ATTCAGCTTTACCTTTTGTACAATTTGAAGTTGTAGCCTATATTTTTGCTT2640.17338876519614604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.17273198957040306No Hit
TTTTTACTCTCTGTATTCTTTCAAATGTTCTGTTTGTCTTTGGTTTTCTTC2540.16682100893871626No Hit
GAATTAAGGAATGAATGAGCAAACAAATAAACCAATGGGTTTAGTTGATTC2540.16682100893871626No Hit
ATATTGTATACATGCAATTTTGCCCTGCAGCTAAAAATATACAATCACCAT2520.1655074576872303No Hit
GCTCTATATTTTGATATAGATCGACAAGAAATTAGTTTTCATTCATGTCTT2480.16288035518425842No Hit
GAAGTAGATTTTTCTTCCTCAAGCTGTCATTTATTTCCCTTTATCTATCCT2430.1595964770555435No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2380.15631259892682864No Hit
TCTTAGAGAAAAGTAAGATGTGAAGGGAATGATAAAACCACTAAAAAAGTG2360.15499904767534267No Hit
GTATATGAAAAAAAAGCTCGACATACTAATCATCATGGAAATGCAAATTAG2360.15499904767534267No Hit
CAGTAGTTGAAAGTATTTATCGTCAGCACATTCACTGTACAAGAGATATTA2340.1536854964238567No Hit
AGGTTATACAAAGTGAGTTCTGAATCCCATTATCTTCCTTCTTTACCTTAT2320.15237194517237077No Hit
CTGTTGTCTTAACATTTACTGTCAAAGGAAAAATGCCTTCTTGATACCATT2290.15040161829514181No Hit
CTCTTAGTTCTTTGTATAGGAGTGATGTTTTTGTTATTCATTCATGATCTA2270.14908806704365588No Hit
TCCTAAACATGTTCAGTTCTTTTTTATTGTGTTTTGGGTATTATCGGTCCT2270.14908806704365588No Hit
GTTGTTTGTTTTCTTATTGTTGAGTTTTAAGAATTCTTTGCATATTTTGGA2250.14777451579216994No Hit
GACAGAAACATAGGAATCTGTGTTATTCTGTAGGATAAACTCAGTTTTTTT2230.14646096454068397No Hit
CTAAAAGAGTATAATTGGATTTGTTTGTAACACAGGGGATAAATGCTTGAG2160.14186353516048314No Hit
GATTATTATGGAACTGAAAAATTCCTATTGCCTAGTAATGTTGTAGCCATT2160.14186353516048314No Hit
TATCTAAATAAGGTGAATTAGGGTAATCCTTTATAAAGTTGCTGATTGCAA2150.14120675953474016No Hit
GGTATGCTTATTAAGTATACTGTAACGCCATTACTTTCGATTCCAATGAAA2140.14054998390899717No Hit
ATATGAACATACATACTTCTTTACATTCCATAGCATTGTATCTGTCTCTTA2120.1392364326575112No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.13792288140602524No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2080.1366093301545393No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2080.1366093301545393No Hit
CATATTGATGAATAATAAAAAGCTCGAAGTAATCTTTGAAATACTGCAAGC1980.13004157389710952No Hit
GTTAAATAATGGACTGAAACAATAAAAATTCCTTCTGGATGGATGTTTTAT1970.12938479827136656No Hit
CTATAAAATCTCACAATCTACTACATTATGCTTTTCTAAAGACCCAAAGAA1970.12938479827136656No Hit
AAATTCCTAAGAAATGTTTCACGATTCTCTCATTCAGAGTAAGGTAGAGTT1950.1280712470198806No Hit
AATTGTTGGTTATACATTTAAGTCACACAGAATTATGGTAAGAATAATGTC1940.1274144713941376No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1930.12675769576839463No Hit
GACTAATCTTATTTTAAACAGCAGGTTAACATTTGTTTTTTCAGATTCATT1920.12610092014265167No Hit
TTTCCATTTAACTTAATTTCACCAAAAGGTTAAAGTAAGAAAGAGTAAAAC1890.12413059326542274No Hit
AAGTTTCTAACACATAAGTTTTGGAGGACACATTCAAAACATAGCACTAAA1890.12413059326542274No Hit
TAGATATTTGTTTCCAATTTCTTCATCCATATGTAGGTGCAAATAACTAAT1880.12347381763967975No Hit
ATGTAGCTATAGTGTGATTTACAATAACTTTGTGTTTTTGTTATCAGCAAG1840.12084671513670786No Hit
TGCAATGATAGACGTAGAAAAAGAAGAAATTCAACATTATTTCCCGGAAAT1800.11821961263373594No Hit
GTAATAATCCAGATATACTCCACGCAGGACTTGTTAAATAGGTAGAAGAAA1790.11756283700799296No Hit
CATAAGAATAATTTAGATTCAACTTCATCGACAAATAATGAAAGTACGGAA1780.11690606138224997No Hit
ACATCATATTGAGCAAAATGCATTTGAAGTAAGTGATAATTTCTATTTCAA1760.11559251013076403No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1750.11493573450502105No Hit
CAGTTATTATAAACTTTGTGTCTGAGAACTCTAATATCTTGGTTAACTGTA1740.11427895887927808No Hit
CAGATGCACATTACTTTCATAGTAGAATTGGAAATTACTGGAATGTGGTTT1740.11427895887927808No Hit
ATCAAATACAGTGTGTATGCCAAGAATAATAATTGCATCAGTAATACCCTC1700.11165185637630616No Hit
GATTAAATCACCTCAAATGAATCTACTTGGTTCAATTTCAAAGTCTCAGAA1690.11099508075056318No Hit
TTGCAAACCAGATAACAGTAATGGGATTTTTCATGTTTTCTTGGTGTAAAT1630.10705442699610532No Hit
GTATTGTTATTAGGTGGCATTCAGTTAATGTCTATTGGTGTAGTTGGCGAA1630.10705442699610532No Hit
ACAATACATTCACCTGTAACAAAAGTGGTTAAATGATCTCAAAATTCACTT1610.10574087574461935No Hit
GTAAAAAAGTTATTATTCAACACCACCATCAAACATGTTCGTTTGAATAGT1610.10574087574461935No Hit
TATTAGAATGAGGGGAATTGGTAAACTATTACAATCACTGAAAACAGCTAT1600.1050841001188764No Hit
GGAACCAACAAGAAGTTTTAGATCTTGAAGAAATGTCAAACACTGAACAAG1600.1050841001188764No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1050841001188764No Hit
AACAAAACTCTTGTAAAAAACTATCTTGCATATCTTCAAGAAAAAGGAATT1600.1050841001188764No Hit
ATTCCTAAGAAGAAATGATAAGATTGTTACGATCATGATTATGAGTACATA1580.10377054886739044No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.10311377324164746No Hit
GCTTTATTGTTTTGCCAGTATCTTAAAGAGGTGACAAATATCTCCATAATC1550.1018002219901615No Hit
TTAGAGAACTTTATAAGCTACTAGAACGAGCTAAAAAGAAGTATCATAAGT1540.10114344636441852No Hit
TAATTATACTTTTGATTGGAAGAGAATGCTGTCTTTTGAAGGTAATACTGG1530.10048667073867554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAC253.8609734E-545.027281
GTCTATG406.712071E-945.0272751
GGTATGC351.1969496E-745.0272751
TTATGGG351.2000419E-745.0124864
GTAGATT253.8684782E-545.0124864
GCTAGGA253.8684782E-545.0124862
CTAGGAC253.8684782E-545.0124863
GAATGGT302.1489577E-645.012485
ATGTCCC406.73208E-945.012485
TAAGCCC207.004414E-445.012486
TGTCCCT406.73208E-945.012486
ATCCTTA207.015764E-444.99770425
ATGGGTG207.015764E-444.99770422
CATGGGT207.015764E-444.99770426
ACTATAC207.015764E-444.99770421
AACCGAT207.015764E-444.99770422
GAAGCCC453.8016879E-1044.99770416
CTGTAGG207.015764E-444.99770428
TTTACGG207.015764E-444.99770435
TGGGTTA207.015764E-444.99770424
Produced by FastQC (version 0.11.2)