FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pb_69.351000000427ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pb_69.351000000427ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198948
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC2090010.505257655266703No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT35401.7793594306049825No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG17090.859018436978507No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4670.23473470454591147No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4660.23423206063896093No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA4290.21563423608179025No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3910.19653376761766894No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2680.13470856706275006No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2620.1316927036210467No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC2280.11460281078472766No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2010.10103142529706256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22600.044.303145
ATGCCGT25950.038.41040844
CTCGTAT28000.035.75892639
TATCTCG28000.035.75892636
ATCTCGT28150.035.6483137
ACTGTAT28100.035.6316732
TCGTATG28050.035.61497540
CGTATGC28100.035.551641
TATGCCG28100.035.551643
TCTCGTA28250.035.4424838
GTATGCC28200.035.42553342
GTATCTC28200.035.42553335
AACTGTA28450.035.1933231
CTCAACT28350.035.1587328
GACTCAA28400.035.0968326
AGACTCA28500.034.97368625
ACTCAAC28500.034.97368627
CCACGAG28700.034.96515720
CGAGACT28600.034.9300723
TCAACTG28650.034.8691129